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The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process.

Jacob A, Parolia A, Pau A, Davamani Amalraj F - BMC Complement Altern Med (2015)

Bottom Line: Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522.Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, International Medical University, Kuala Lumpur, Malaysia. annjacob.aj90@gmail.com.

ABSTRACT

Background: To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.

Methods: Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α=0.05).

Results: Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

Conclusion: Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

No MeSH data available.


Related in: MedlinePlus

Microscopic images of wound areas in cells treated with different concentrations of Malaysian propolis at various time points. The experiment was carried out in triplicates. Images were taken at 4× magnification of the objective lens and the scale bars indicate a distance of 100 μm
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Fig1: Microscopic images of wound areas in cells treated with different concentrations of Malaysian propolis at various time points. The experiment was carried out in triplicates. Images were taken at 4× magnification of the objective lens and the scale bars indicate a distance of 100 μm

Mentions: Malaysian propolis at concentrations of 1, 10 and 250 μg/mL indicated visually faster wound closure compared to the control, while the other concentrations appeared visually similar to the control. The ability of cells to migrate into wounds in the presence of DMSO was similar to control at 12 h, but at 24 and 48 h the migration rate was slower than all other groups (Fig. 1).Fig. 1


The effects of Malaysian propolis and Brazilian red propolis on connective tissue fibroblasts in the wound healing process.

Jacob A, Parolia A, Pau A, Davamani Amalraj F - BMC Complement Altern Med (2015)

Microscopic images of wound areas in cells treated with different concentrations of Malaysian propolis at various time points. The experiment was carried out in triplicates. Images were taken at 4× magnification of the objective lens and the scale bars indicate a distance of 100 μm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4549008&req=5

Fig1: Microscopic images of wound areas in cells treated with different concentrations of Malaysian propolis at various time points. The experiment was carried out in triplicates. Images were taken at 4× magnification of the objective lens and the scale bars indicate a distance of 100 μm
Mentions: Malaysian propolis at concentrations of 1, 10 and 250 μg/mL indicated visually faster wound closure compared to the control, while the other concentrations appeared visually similar to the control. The ability of cells to migrate into wounds in the presence of DMSO was similar to control at 12 h, but at 24 and 48 h the migration rate was slower than all other groups (Fig. 1).Fig. 1

Bottom Line: Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522.Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, International Medical University, Kuala Lumpur, Malaysia. annjacob.aj90@gmail.com.

ABSTRACT

Background: To evaluate and compare the effects of ethanolic extracts of Malaysian propolis and Brazilian red propolis at different concentrations on the migration and proliferation of fibroblast cells.

Methods: Malaysian and Brazilian red propolis crude samples were extracted using ethanol. Their wound healing effects were tested in vitro on the normal human fibroblast cell line CRL-7522. Cell migration and proliferation assays were carried out using propolis concentrations of 1, 10, 100, 250, 500 and 1000 μg/mL. The data were analyzed using one-way ANOVA and post hoc Bonferroni tests (α=0.05).

Results: Malaysian and Brazilian red propolis followed a concentration-dependent increasing and decreasing trend. Malaysian propolis showed the fastest migration rate at 250 μg/mL which was statistically significant (p<0.05) and maximum proliferation at 500 μg/mL with no significant difference (p>0.05) compared to control. Brazilian red propolis showed a slight increase in migration and proliferation at 10 and 100 μg/mL, respectively with no significant difference (p>0.05) compared to control, while concentrations above these conferred inhibitory effects.

Conclusion: Malaysian and Brazilian red propolis show potential to assist in wound healing, depending on their concentration.

No MeSH data available.


Related in: MedlinePlus