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Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs.

Ridenour C, Johnson A, Winne E, Hossain J, Mateu-Petit G, Balish A, Santana W, Kim T, Davis C, Cox NJ, Barr JR, Donis RO, Villanueva J, Williams TL, Chen LM - Influenza Other Respir Viruses (2015)

Bottom Line: The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera.However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.

View Article: PubMed Central - PubMed

Affiliation: Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

No MeSH data available.


Related in: MedlinePlus

Yield analysis of second-generation reassortant viruses derived from parental CVV. (A) Quantification of total viral protein, shown as mg total viral protein/100 eggs (B) Quantification of HA antigen, shown as mg HA/100 eggs. Values shown are the average of at least two independent experiments with errors bars denoting standard deviation.
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fig03: Yield analysis of second-generation reassortant viruses derived from parental CVV. (A) Quantification of total viral protein, shown as mg total viral protein/100 eggs (B) Quantification of HA antigen, shown as mg HA/100 eggs. Values shown are the average of at least two independent experiments with errors bars denoting standard deviation.

Mentions: The total viral protein yield of sucrose gradient-purified samples from the second-generation viruses ranged from 9 to 21 mg protein/100 eggs (Figure3A), with HA yields of 4–9 mg/100 eggs quantified through IDMS (Figure3B). Notably, three of the six viruses, IDCDC-RG32A.2, IDCDC-RG32A.3, and IDCDC-RG32B.4, achieved total viral protein and HA antigen yields comparable to those of the seasonal high growth reassortant, X-181A (H1N1)pdm09. The HA/NP and HA/M1 molar ratios from all egg-passaged viruses remained similar to the HA/NP and HA/M1 ratios of their respective parental virus (Table2).


Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs.

Ridenour C, Johnson A, Winne E, Hossain J, Mateu-Petit G, Balish A, Santana W, Kim T, Davis C, Cox NJ, Barr JR, Donis RO, Villanueva J, Williams TL, Chen LM - Influenza Other Respir Viruses (2015)

Yield analysis of second-generation reassortant viruses derived from parental CVV. (A) Quantification of total viral protein, shown as mg total viral protein/100 eggs (B) Quantification of HA antigen, shown as mg HA/100 eggs. Values shown are the average of at least two independent experiments with errors bars denoting standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4548996&req=5

fig03: Yield analysis of second-generation reassortant viruses derived from parental CVV. (A) Quantification of total viral protein, shown as mg total viral protein/100 eggs (B) Quantification of HA antigen, shown as mg HA/100 eggs. Values shown are the average of at least two independent experiments with errors bars denoting standard deviation.
Mentions: The total viral protein yield of sucrose gradient-purified samples from the second-generation viruses ranged from 9 to 21 mg protein/100 eggs (Figure3A), with HA yields of 4–9 mg/100 eggs quantified through IDMS (Figure3B). Notably, three of the six viruses, IDCDC-RG32A.2, IDCDC-RG32A.3, and IDCDC-RG32B.4, achieved total viral protein and HA antigen yields comparable to those of the seasonal high growth reassortant, X-181A (H1N1)pdm09. The HA/NP and HA/M1 molar ratios from all egg-passaged viruses remained similar to the HA/NP and HA/M1 ratios of their respective parental virus (Table2).

Bottom Line: The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.CVVs were antigenically characterized by hemagglutination inhibition (HI) assays with ferret antisera.However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.

View Article: PubMed Central - PubMed

Affiliation: Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

No MeSH data available.


Related in: MedlinePlus