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Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs.

Ridenour C, Johnson A, Winne E, Hossain J, Mateu-Petit G, Balish A, Santana W, Kim T, Davis C, Cox NJ, Barr JR, Donis RO, Villanueva J, Williams TL, Chen LM - Influenza Other Respir Viruses (2015)

Bottom Line: The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield.However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.

View Article: PubMed Central - PubMed

Affiliation: Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

No MeSH data available.


Related in: MedlinePlus

Yield analysis of second-generation reassortant viruses derived from parental CVV. (A) Quantification of total viral protein, shown as mg total viral protein/100 eggs (B) Quantification of HA antigen, shown as mg HA/100 eggs. Values shown are the average of at least two independent experiments with errors bars denoting standard deviation.
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fig03: Yield analysis of second-generation reassortant viruses derived from parental CVV. (A) Quantification of total viral protein, shown as mg total viral protein/100 eggs (B) Quantification of HA antigen, shown as mg HA/100 eggs. Values shown are the average of at least two independent experiments with errors bars denoting standard deviation.

Mentions: The total viral protein yield of sucrose gradient-purified samples from the second-generation viruses ranged from 9 to 21 mg protein/100 eggs (Figure3A), with HA yields of 4–9 mg/100 eggs quantified through IDMS (Figure3B). Notably, three of the six viruses, IDCDC-RG32A.2, IDCDC-RG32A.3, and IDCDC-RG32B.4, achieved total viral protein and HA antigen yields comparable to those of the seasonal high growth reassortant, X-181A (H1N1)pdm09. The HA/NP and HA/M1 molar ratios from all egg-passaged viruses remained similar to the HA/NP and HA/M1 ratios of their respective parental virus (Table2).


Development of influenza A(H7N9) candidate vaccine viruses with improved hemagglutinin antigen yield in eggs.

Ridenour C, Johnson A, Winne E, Hossain J, Mateu-Petit G, Balish A, Santana W, Kim T, Davis C, Cox NJ, Barr JR, Donis RO, Villanueva J, Williams TL, Chen LM - Influenza Other Respir Viruses (2015)

Yield analysis of second-generation reassortant viruses derived from parental CVV. (A) Quantification of total viral protein, shown as mg total viral protein/100 eggs (B) Quantification of HA antigen, shown as mg HA/100 eggs. Values shown are the average of at least two independent experiments with errors bars denoting standard deviation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4548996&req=5

fig03: Yield analysis of second-generation reassortant viruses derived from parental CVV. (A) Quantification of total viral protein, shown as mg total viral protein/100 eggs (B) Quantification of HA antigen, shown as mg HA/100 eggs. Values shown are the average of at least two independent experiments with errors bars denoting standard deviation.
Mentions: The total viral protein yield of sucrose gradient-purified samples from the second-generation viruses ranged from 9 to 21 mg protein/100 eggs (Figure3A), with HA yields of 4–9 mg/100 eggs quantified through IDMS (Figure3B). Notably, three of the six viruses, IDCDC-RG32A.2, IDCDC-RG32A.3, and IDCDC-RG32B.4, achieved total viral protein and HA antigen yields comparable to those of the seasonal high growth reassortant, X-181A (H1N1)pdm09. The HA/NP and HA/M1 molar ratios from all egg-passaged viruses remained similar to the HA/NP and HA/M1 ratios of their respective parental virus (Table2).

Bottom Line: The resulting A(H7N9) CVVs needed improvement because they had titers and antigen yields that were suboptimal for vaccine manufacturing in eggs, especially in a pandemic situation.Two CVVs derived by reverse genetics were serially passaged in embryonated eggs to improve the hemagglutinin (HA) antigen yield.However, HI tests indicated that the antigenic properties of two CVVs remained unchanged.

View Article: PubMed Central - PubMed

Affiliation: Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.

No MeSH data available.


Related in: MedlinePlus