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Two New Species of Liuixalus (Rhacophoridae, Anura): Evidence from Morphological and Molecular Analyses.

Qin S, Mo Y, Jiang K, Cai B, Xie F, Jiang J, Murphy RW, Li JT, Wang Y - PLoS ONE (2015)

Bottom Line: Dayao, Jinxiu, China and Liuixalus shiwandashan sp. nov. from the type locality Mt.Shiwanda, China.A combination of morphological measurements, genetic, bioacoustic and osteological analysis was served to diagnose the new taxa.

View Article: PubMed Central - PubMed

Affiliation: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China; College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.

ABSTRACT
Due to small body sizes, superficial similarities in morphologies, and obscure activity behaviors, the phylogeny and taxonomy of species in the genus Liuixalus were very troublesome. Some species might comprise a complex of cryptic species. To investigate the species of group, we constructed the matrilineal genealogy of the genus using 16s rRNA mitochondrial DNA sequences. Analyses recovered six well supported matrilines that involved L. romeri, L. ocellatus, L. hainanus, L. calcarius, Liuixalus shiwandashan sp. nov. and Liuixalus jinxiuensis sp. nov., though the historical relationships among them remained unresolved. Currently, Liuixalus included 4 species, distributed eastwards from northern Vietnam to Hong Kong, China. Based on genealogical and morphological distinctiveness, we described Liuixalus jinxiuensis sp. nov. from the type locality Mt. Dayao, Jinxiu, China and Liuixalus shiwandashan sp. nov. from the type locality Mt. Shiwanda, China. A combination of morphological measurements, genetic, bioacoustic and osteological analysis was served to diagnose the new taxa.

No MeSH data available.


Collection sites of the species in this study.
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pone.0136134.g011: Collection sites of the species in this study.

Mentions: The localities of these species were marked in the map (Fig 11), and they were well separated geographically. The close relationships among Liuixalus suggested an ancient link between Hainan, Guangxi, Hong Kong and Vietnam. According to the geological evidence, the separation between Hainan Island and mainland China occurred at Early Cretaceous Epoch [31–32]. The separation accelerated the speciation within the genus Liuixalus.


Two New Species of Liuixalus (Rhacophoridae, Anura): Evidence from Morphological and Molecular Analyses.

Qin S, Mo Y, Jiang K, Cai B, Xie F, Jiang J, Murphy RW, Li JT, Wang Y - PLoS ONE (2015)

Collection sites of the species in this study.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4548945&req=5

pone.0136134.g011: Collection sites of the species in this study.
Mentions: The localities of these species were marked in the map (Fig 11), and they were well separated geographically. The close relationships among Liuixalus suggested an ancient link between Hainan, Guangxi, Hong Kong and Vietnam. According to the geological evidence, the separation between Hainan Island and mainland China occurred at Early Cretaceous Epoch [31–32]. The separation accelerated the speciation within the genus Liuixalus.

Bottom Line: Dayao, Jinxiu, China and Liuixalus shiwandashan sp. nov. from the type locality Mt.Shiwanda, China.A combination of morphological measurements, genetic, bioacoustic and osteological analysis was served to diagnose the new taxa.

View Article: PubMed Central - PubMed

Affiliation: Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu, Sichuan, China; College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.

ABSTRACT
Due to small body sizes, superficial similarities in morphologies, and obscure activity behaviors, the phylogeny and taxonomy of species in the genus Liuixalus were very troublesome. Some species might comprise a complex of cryptic species. To investigate the species of group, we constructed the matrilineal genealogy of the genus using 16s rRNA mitochondrial DNA sequences. Analyses recovered six well supported matrilines that involved L. romeri, L. ocellatus, L. hainanus, L. calcarius, Liuixalus shiwandashan sp. nov. and Liuixalus jinxiuensis sp. nov., though the historical relationships among them remained unresolved. Currently, Liuixalus included 4 species, distributed eastwards from northern Vietnam to Hong Kong, China. Based on genealogical and morphological distinctiveness, we described Liuixalus jinxiuensis sp. nov. from the type locality Mt. Dayao, Jinxiu, China and Liuixalus shiwandashan sp. nov. from the type locality Mt. Shiwanda, China. A combination of morphological measurements, genetic, bioacoustic and osteological analysis was served to diagnose the new taxa.

No MeSH data available.