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Neuroprotective effect of Spirulina fusiform and amantadine in the 6-OHDA induced Parkinsonism in rats.

Chattopadhyaya I, Gupta S, Mohammed A, Mushtaq N, Chauhan S, Ghosh S, Ghosh S - BMC Complement Altern Med (2015)

Bottom Line: Both the body rotations (ipsilateral and contralateral) were found to have a statistically significant (p<0.001) decrease by 34.26 and 52% after treatment with spirulina (Twice a day) in spirulina treated lesioned group.A higher percentage of improvement was shown in the reduction of ipsilateral (57.34%) and contralateral (78.3%) rotations in combination of spirulina with amantadine treated lesioned group rather than spirulina alone treated lesioned groups when compared with positive control lesioned group.Spirulina is a potent nutraceutical supplement all over the world, so my preclinical study may contribute to give an additional adjuvant drug therapy in aging related disorders (Neurodegenerative as well as diabetes associated neurodegenerative disorders).

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, M. M. College of Pharmacy, M. M. University, Mullana, (Ambala), Haryana, India.

ABSTRACT

Background: Multi-factorial etiology exists in pathophysiology of neurodegenerative diseases. The imbalance of anti-oxidant enzymes and dopamine level leads to Parkinsonism. The objective of this study was to assess the protective effect of Spirulina fusiform alone and in combination with amantadine against Parkinsonism effect in 6-hydroxydopamine (6-OHDA) induced rat model.

Methods: S. fusiform was administered in different groups (500 mg/kg, once daily and twice daily) and a combination of spirulina (500 mg/kg, once daily) with amantadine (20 mg/kg once daily) for 30 days before and 14 days after a single injection of 6-OHDA into the dorsal striatum. Post lesion produced rotational behavior which was measured at two week intervals (37th and 44th day). Locomotors activity was also done at 44th and muscle coordination at 48th day. Dorsal striatum was isolated from rat brain for evaluating the antioxidant assays and dopamine content at 49th day.

Results: Both the body rotations (ipsilateral and contralateral) were found to have a statistically significant (p<0.001) decrease by 34.26 and 52% after treatment with spirulina (Twice a day) in spirulina treated lesioned group. A higher percentage of improvement was shown in the reduction of ipsilateral (57.34%) and contralateral (78.3%) rotations in combination of spirulina with amantadine treated lesioned group rather than spirulina alone treated lesioned groups when compared with positive control lesioned group. Body movements and locomotor activity were improved statistically (p<0.0001) significant in both treated lesioned groups (Combination of spirulina with amantadine and spirulina twice daily). Similar results were also seen in anti-oxidant levels which later on reached to the normal value. The levels of dopamine content had a statistically significant (p<0.0001) increase by 78.3% only in case of spirulina with amantadine treated lesioned group.

Conclusion: Spirulina is a potent nutraceutical supplement all over the world, so my preclinical study may contribute to give an additional adjuvant drug therapy in aging related disorders (Neurodegenerative as well as diabetes associated neurodegenerative disorders).

No MeSH data available.


Related in: MedlinePlus

The effect of treatment on stereotype. Each bar represents the mean ± standard deviation of six animals and the experiments were repeated twice. ap < 0.001 vs Saline sham operated, *p < 0.05,**p < 0.01,***p < 0.001 VS Lesioned positive control operated
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Fig4: The effect of treatment on stereotype. Each bar represents the mean ± standard deviation of six animals and the experiments were repeated twice. ap < 0.001 vs Saline sham operated, *p < 0.05,**p < 0.01,***p < 0.001 VS Lesioned positive control operated

Mentions: Among all lesioned drug treated groups (Fig. 3), spirulina administered twice daily and spirulina with amantadine showed almost 33 and 55 % improvement in the path covered by rats and showed statistically (p < 0.001) significant difference when compared with lesioned positive control group declined in path covered (68.78 %). Similar results in case of stereotype events (Fig. 4), treatment of spirulina with amantadine combination exhibited statistically (p < 0.001) significant enhancement (78.58 %) when compared with lesioned positive control group (↓76.68 %). Both spirulina treated lesioned groups showed a dose dependent effect statistically (Spirulina 500 mg/kg once daily 24 %, p < 0.01; spirulina 500 mg/kg twice daily 48.4 %, p < 0.001).Fig. 3


Neuroprotective effect of Spirulina fusiform and amantadine in the 6-OHDA induced Parkinsonism in rats.

Chattopadhyaya I, Gupta S, Mohammed A, Mushtaq N, Chauhan S, Ghosh S, Ghosh S - BMC Complement Altern Med (2015)

The effect of treatment on stereotype. Each bar represents the mean ± standard deviation of six animals and the experiments were repeated twice. ap < 0.001 vs Saline sham operated, *p < 0.05,**p < 0.01,***p < 0.001 VS Lesioned positive control operated
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4548915&req=5

Fig4: The effect of treatment on stereotype. Each bar represents the mean ± standard deviation of six animals and the experiments were repeated twice. ap < 0.001 vs Saline sham operated, *p < 0.05,**p < 0.01,***p < 0.001 VS Lesioned positive control operated
Mentions: Among all lesioned drug treated groups (Fig. 3), spirulina administered twice daily and spirulina with amantadine showed almost 33 and 55 % improvement in the path covered by rats and showed statistically (p < 0.001) significant difference when compared with lesioned positive control group declined in path covered (68.78 %). Similar results in case of stereotype events (Fig. 4), treatment of spirulina with amantadine combination exhibited statistically (p < 0.001) significant enhancement (78.58 %) when compared with lesioned positive control group (↓76.68 %). Both spirulina treated lesioned groups showed a dose dependent effect statistically (Spirulina 500 mg/kg once daily 24 %, p < 0.01; spirulina 500 mg/kg twice daily 48.4 %, p < 0.001).Fig. 3

Bottom Line: Both the body rotations (ipsilateral and contralateral) were found to have a statistically significant (p<0.001) decrease by 34.26 and 52% after treatment with spirulina (Twice a day) in spirulina treated lesioned group.A higher percentage of improvement was shown in the reduction of ipsilateral (57.34%) and contralateral (78.3%) rotations in combination of spirulina with amantadine treated lesioned group rather than spirulina alone treated lesioned groups when compared with positive control lesioned group.Spirulina is a potent nutraceutical supplement all over the world, so my preclinical study may contribute to give an additional adjuvant drug therapy in aging related disorders (Neurodegenerative as well as diabetes associated neurodegenerative disorders).

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, M. M. College of Pharmacy, M. M. University, Mullana, (Ambala), Haryana, India.

ABSTRACT

Background: Multi-factorial etiology exists in pathophysiology of neurodegenerative diseases. The imbalance of anti-oxidant enzymes and dopamine level leads to Parkinsonism. The objective of this study was to assess the protective effect of Spirulina fusiform alone and in combination with amantadine against Parkinsonism effect in 6-hydroxydopamine (6-OHDA) induced rat model.

Methods: S. fusiform was administered in different groups (500 mg/kg, once daily and twice daily) and a combination of spirulina (500 mg/kg, once daily) with amantadine (20 mg/kg once daily) for 30 days before and 14 days after a single injection of 6-OHDA into the dorsal striatum. Post lesion produced rotational behavior which was measured at two week intervals (37th and 44th day). Locomotors activity was also done at 44th and muscle coordination at 48th day. Dorsal striatum was isolated from rat brain for evaluating the antioxidant assays and dopamine content at 49th day.

Results: Both the body rotations (ipsilateral and contralateral) were found to have a statistically significant (p<0.001) decrease by 34.26 and 52% after treatment with spirulina (Twice a day) in spirulina treated lesioned group. A higher percentage of improvement was shown in the reduction of ipsilateral (57.34%) and contralateral (78.3%) rotations in combination of spirulina with amantadine treated lesioned group rather than spirulina alone treated lesioned groups when compared with positive control lesioned group. Body movements and locomotor activity were improved statistically (p<0.0001) significant in both treated lesioned groups (Combination of spirulina with amantadine and spirulina twice daily). Similar results were also seen in anti-oxidant levels which later on reached to the normal value. The levels of dopamine content had a statistically significant (p<0.0001) increase by 78.3% only in case of spirulina with amantadine treated lesioned group.

Conclusion: Spirulina is a potent nutraceutical supplement all over the world, so my preclinical study may contribute to give an additional adjuvant drug therapy in aging related disorders (Neurodegenerative as well as diabetes associated neurodegenerative disorders).

No MeSH data available.


Related in: MedlinePlus