Limits...
Bifidobacteria grown on human milk oligosaccharides downregulate the expression of inflammation-related genes in Caco-2 cells.

Wickramasinghe S, Pacheco AR, Lemay DG, Mills DA - BMC Microbiol. (2015)

Bottom Line: Despite differential binding ability, both HMO-grown strains significantly differentially affected the Caco-2 transcriptome compared to their glucose or lactose grown controls.HMO-grown B. breve and B. infantis both downregulated genes in Caco-2 cells associated with chemokine activity.HMO-grown bifidobacteria reduce markers of inflammation, compared to glucose or lactose-grown bifidobacteria.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Veterinary Sciences, University of Peradeniya, Peradeniya, 20400, Sri Lanka. saumyawicks@gmail.com.

ABSTRACT

Background: Breastfed human infants are predominantly colonized by bifidobacteria that thrive on human milk oligosaccharides (HMO). Two predominant species of bifidobacteria in infant feces are Bifidobacterium breve (B. breve) and Bifidobacterium longum subsp. infantis (B. infantis), both of which include avid HMO-consumer strains. Our laboratory has previously shown that B. infantis, when grown on HMO, increases adhesion to intestinal cells and increases the expression of the anti-inflammatory cytokine interleukin-10. The purpose of the current study was to investigate the effects of carbon source-glucose, lactose, or HMO-on the ability of B. breve and B. infantis to adhere to and affect the transcription of intestinal epithelial cells on a genome-wide basis.

Results: HMO-grown B. infantis had higher percent binding to Caco-2 cell monolayers compared to B. infantis grown on glucose or lactose. B. breve had low adhesive ability regardless of carbon source. Despite differential binding ability, both HMO-grown strains significantly differentially affected the Caco-2 transcriptome compared to their glucose or lactose grown controls. HMO-grown B. breve and B. infantis both downregulated genes in Caco-2 cells associated with chemokine activity.

Conclusion: The choice of carbon source affects the interaction of bifidobacteria with intestinal epithelial cells. HMO-grown bifidobacteria reduce markers of inflammation, compared to glucose or lactose-grown bifidobacteria. In the future, the design of preventative or therapeutic probiotic supplements may need to include appropriately chosen prebiotics.

No MeSH data available.


Related in: MedlinePlus

Number of Caco-2 genes differentially expressed between B. infantis ATCC 15697 and B. breve SC95 in response to different substrates (GLU = glucose, LAC = lactose, HMO = human milk oligosaccharides). Venn diagram prepared using EulerAPE [62]
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4548914&req=5

Fig2: Number of Caco-2 genes differentially expressed between B. infantis ATCC 15697 and B. breve SC95 in response to different substrates (GLU = glucose, LAC = lactose, HMO = human milk oligosaccharides). Venn diagram prepared using EulerAPE [62]

Mentions: To compare the differential effects of bifidobacterial strain and carbon source on the Caco-2 cell transcriptome, between-strain differential gene expression was examined for each of the three carbon sources. The number of Caco-2 genes differentially expressed in response to co-incubation with B. infantis compared with B. breve for each carbon source is listed in Fig. 2. For example, there are 2596 Caco-2 genes that are differentially expressed in response to GLU-fed B. infantis compared to GLU-fed B. breve that are not also differentially expressed in response to HMO- or LAC-fed strains. The carbon source of glucose elicits the biggest bifidobacteria strain effect on Caco-2 gene expression. Meanwhile, the use of HMO as a carbon source appears to reduce the difference between strains. If the choice of carbon source were unimportant, the intersection of the gene expression results (Fig. 2) should be quite high. However, that is not the case. The intersection of the three carbon sources is rather small (Fig. 2), suggesting that the choice of carbon source definitively affects Caco-2 response to different bifidobacteria strains. Thus, the differential response of Caco-2 cells to the two different bifidobacteria strains is highly dependent on the carbon source.Fig. 2


Bifidobacteria grown on human milk oligosaccharides downregulate the expression of inflammation-related genes in Caco-2 cells.

Wickramasinghe S, Pacheco AR, Lemay DG, Mills DA - BMC Microbiol. (2015)

Number of Caco-2 genes differentially expressed between B. infantis ATCC 15697 and B. breve SC95 in response to different substrates (GLU = glucose, LAC = lactose, HMO = human milk oligosaccharides). Venn diagram prepared using EulerAPE [62]
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4548914&req=5

Fig2: Number of Caco-2 genes differentially expressed between B. infantis ATCC 15697 and B. breve SC95 in response to different substrates (GLU = glucose, LAC = lactose, HMO = human milk oligosaccharides). Venn diagram prepared using EulerAPE [62]
Mentions: To compare the differential effects of bifidobacterial strain and carbon source on the Caco-2 cell transcriptome, between-strain differential gene expression was examined for each of the three carbon sources. The number of Caco-2 genes differentially expressed in response to co-incubation with B. infantis compared with B. breve for each carbon source is listed in Fig. 2. For example, there are 2596 Caco-2 genes that are differentially expressed in response to GLU-fed B. infantis compared to GLU-fed B. breve that are not also differentially expressed in response to HMO- or LAC-fed strains. The carbon source of glucose elicits the biggest bifidobacteria strain effect on Caco-2 gene expression. Meanwhile, the use of HMO as a carbon source appears to reduce the difference between strains. If the choice of carbon source were unimportant, the intersection of the gene expression results (Fig. 2) should be quite high. However, that is not the case. The intersection of the three carbon sources is rather small (Fig. 2), suggesting that the choice of carbon source definitively affects Caco-2 response to different bifidobacteria strains. Thus, the differential response of Caco-2 cells to the two different bifidobacteria strains is highly dependent on the carbon source.Fig. 2

Bottom Line: Despite differential binding ability, both HMO-grown strains significantly differentially affected the Caco-2 transcriptome compared to their glucose or lactose grown controls.HMO-grown B. breve and B. infantis both downregulated genes in Caco-2 cells associated with chemokine activity.HMO-grown bifidobacteria reduce markers of inflammation, compared to glucose or lactose-grown bifidobacteria.

View Article: PubMed Central - PubMed

Affiliation: Department of Basic Veterinary Sciences, University of Peradeniya, Peradeniya, 20400, Sri Lanka. saumyawicks@gmail.com.

ABSTRACT

Background: Breastfed human infants are predominantly colonized by bifidobacteria that thrive on human milk oligosaccharides (HMO). Two predominant species of bifidobacteria in infant feces are Bifidobacterium breve (B. breve) and Bifidobacterium longum subsp. infantis (B. infantis), both of which include avid HMO-consumer strains. Our laboratory has previously shown that B. infantis, when grown on HMO, increases adhesion to intestinal cells and increases the expression of the anti-inflammatory cytokine interleukin-10. The purpose of the current study was to investigate the effects of carbon source-glucose, lactose, or HMO-on the ability of B. breve and B. infantis to adhere to and affect the transcription of intestinal epithelial cells on a genome-wide basis.

Results: HMO-grown B. infantis had higher percent binding to Caco-2 cell monolayers compared to B. infantis grown on glucose or lactose. B. breve had low adhesive ability regardless of carbon source. Despite differential binding ability, both HMO-grown strains significantly differentially affected the Caco-2 transcriptome compared to their glucose or lactose grown controls. HMO-grown B. breve and B. infantis both downregulated genes in Caco-2 cells associated with chemokine activity.

Conclusion: The choice of carbon source affects the interaction of bifidobacteria with intestinal epithelial cells. HMO-grown bifidobacteria reduce markers of inflammation, compared to glucose or lactose-grown bifidobacteria. In the future, the design of preventative or therapeutic probiotic supplements may need to include appropriately chosen prebiotics.

No MeSH data available.


Related in: MedlinePlus