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Plectin as a prognostic marker in non-metastatic oral squamous cell carcinoma.

Rikardsen OG, Magnussen SN, Svineng G, Hadler-Olsen E, Uhlin-Hansen L, Steigen SE - BMC Oral Health (2015)

Bottom Line: Oral squamous cell carcinoma (OSCC) is associated with a poor 5-year survival rate.The expression of plectin was compared with clinicopathological variables and 5 year survival.Our results indicate that low expression of plectin predicts a favorable outcome for patients with non-metastatic OSCC and the expression level of plectin may therefore be used in the treatment stratification for patients with early stage disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, University Hospital of North Norway, Tromsø, Norway. oddveig.rikardsen@unn.no.

ABSTRACT

Background: Oral squamous cell carcinoma (OSCC) is associated with a poor 5-year survival rate. In general, patients diagnosed with small tumors have a fairly good prognosis, but some small tumors have an aggressive behavior leading to early death. There are at present no reliable prognostic biomarkers for oral cancers. Thus, to optimize treatment for the individual patient, there is a need for biomarkers that can predict tumor behavior.

Method: In the present study the potential prognostic value of plectin was evaluated by a tissue microarray (TMA) based immunohistochemical analysis of primary tumor tissue obtained from a North Norwegian cohort of 115 patients diagnosed with OSCC. The expression of plectin was compared with clinicopathological variables and 5 year survival.

Results: The statistical analysis revealed that low expression of plectin in the tumor cells predicted a favorable outcome for patients with non-metastatic disease (p = 0.008). Furthermore, the expression of plectin was found to correlate (p = 0.01) with the expression of uPAR, which we have previously found to be a potential prognostic marker for T1N0 tumors.

Conclusions: Our results indicate that low expression of plectin predicts a favorable outcome for patients with non-metastatic OSCC and the expression level of plectin may therefore be used in the treatment stratification for patients with early stage disease.

No MeSH data available.


Related in: MedlinePlus

Immunofluorescence staining of oral squamous cell carcinoma tissue. The majority of the cells were positive for both plectin and uPAR. Plectin (green) is located mainly at the plasma membrane and the periphery of the cell, while uPAR (red) is more prominent in the cytoplasmic part of the cells. Plectin is highly expressed in the wall of blood vessels (asterix)
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Fig5: Immunofluorescence staining of oral squamous cell carcinoma tissue. The majority of the cells were positive for both plectin and uPAR. Plectin (green) is located mainly at the plasma membrane and the periphery of the cell, while uPAR (red) is more prominent in the cytoplasmic part of the cells. Plectin is highly expressed in the wall of blood vessels (asterix)

Mentions: We have recently shown that patients with T1N0 tumors expressing low levels of uPAR have a significantly reduced DSD compared to those with a higher uPAR expression. To investigate the distribution of expression of uPAR and plectin in the tumors we did a chi-square test, and found that a significant number of the tumors that were high expressers of uPAR also were high expressers of plectin, and those with low expression of uPAR generally were low expressers of plectin. This correlation was significant with a correlation coefficient of 0.769 (p = 0.01) as shown in a scatter plot in Fig. 4. Double immunofluorescence staining was performed on some selected tumors to investigate whether plectin and uPAR were co-located within the same cells, co-expressed by the same cells, or located to the same tumor regions. The immunofluorescence showed that the plectin and uPAR staining was localized to the same areas of the tumor (Fig. 5). As expected, the immunofluorescence staining confirmed that plectin was found mainly at the plasma membrane while uPAR was mainly localized in the cytoplasm.Fig. 4


Plectin as a prognostic marker in non-metastatic oral squamous cell carcinoma.

Rikardsen OG, Magnussen SN, Svineng G, Hadler-Olsen E, Uhlin-Hansen L, Steigen SE - BMC Oral Health (2015)

Immunofluorescence staining of oral squamous cell carcinoma tissue. The majority of the cells were positive for both plectin and uPAR. Plectin (green) is located mainly at the plasma membrane and the periphery of the cell, while uPAR (red) is more prominent in the cytoplasmic part of the cells. Plectin is highly expressed in the wall of blood vessels (asterix)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4548848&req=5

Fig5: Immunofluorescence staining of oral squamous cell carcinoma tissue. The majority of the cells were positive for both plectin and uPAR. Plectin (green) is located mainly at the plasma membrane and the periphery of the cell, while uPAR (red) is more prominent in the cytoplasmic part of the cells. Plectin is highly expressed in the wall of blood vessels (asterix)
Mentions: We have recently shown that patients with T1N0 tumors expressing low levels of uPAR have a significantly reduced DSD compared to those with a higher uPAR expression. To investigate the distribution of expression of uPAR and plectin in the tumors we did a chi-square test, and found that a significant number of the tumors that were high expressers of uPAR also were high expressers of plectin, and those with low expression of uPAR generally were low expressers of plectin. This correlation was significant with a correlation coefficient of 0.769 (p = 0.01) as shown in a scatter plot in Fig. 4. Double immunofluorescence staining was performed on some selected tumors to investigate whether plectin and uPAR were co-located within the same cells, co-expressed by the same cells, or located to the same tumor regions. The immunofluorescence showed that the plectin and uPAR staining was localized to the same areas of the tumor (Fig. 5). As expected, the immunofluorescence staining confirmed that plectin was found mainly at the plasma membrane while uPAR was mainly localized in the cytoplasm.Fig. 4

Bottom Line: Oral squamous cell carcinoma (OSCC) is associated with a poor 5-year survival rate.The expression of plectin was compared with clinicopathological variables and 5 year survival.Our results indicate that low expression of plectin predicts a favorable outcome for patients with non-metastatic OSCC and the expression level of plectin may therefore be used in the treatment stratification for patients with early stage disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, University Hospital of North Norway, Tromsø, Norway. oddveig.rikardsen@unn.no.

ABSTRACT

Background: Oral squamous cell carcinoma (OSCC) is associated with a poor 5-year survival rate. In general, patients diagnosed with small tumors have a fairly good prognosis, but some small tumors have an aggressive behavior leading to early death. There are at present no reliable prognostic biomarkers for oral cancers. Thus, to optimize treatment for the individual patient, there is a need for biomarkers that can predict tumor behavior.

Method: In the present study the potential prognostic value of plectin was evaluated by a tissue microarray (TMA) based immunohistochemical analysis of primary tumor tissue obtained from a North Norwegian cohort of 115 patients diagnosed with OSCC. The expression of plectin was compared with clinicopathological variables and 5 year survival.

Results: The statistical analysis revealed that low expression of plectin in the tumor cells predicted a favorable outcome for patients with non-metastatic disease (p = 0.008). Furthermore, the expression of plectin was found to correlate (p = 0.01) with the expression of uPAR, which we have previously found to be a potential prognostic marker for T1N0 tumors.

Conclusions: Our results indicate that low expression of plectin predicts a favorable outcome for patients with non-metastatic OSCC and the expression level of plectin may therefore be used in the treatment stratification for patients with early stage disease.

No MeSH data available.


Related in: MedlinePlus