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Distinctive and pervasive alterations in aqueous humor protein composition following different types of glaucoma surgery.

Rosenfeld C, Price MO, Lai X, Witzmann FA, Price FW - Mol. Vis. (2015)

Bottom Line: To investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.Relative protein quantities were compared across groups with ANOVA.Different glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: Cornea Research Foundation of America, Indianapolis, IN.

ABSTRACT

Purpose: To investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.

Methods: In this cross-sectional study, aqueous humor samples were prospectively collected from control subjects who underwent routine cataract surgery (n=14) and from patients who had different glaucoma filters: Baerveldt aqueous shunt (n=6), Ahmed aqueous shunt (n=6), trabeculectomy (n=5), and Ex-Press trabeculectomy (n=3). Total protein concentrations were determined with Bradford assay. Tryptic digests were analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Proteins were identified with high confidence using stringent criteria and were quantitatively compared with a label-free platform. Relative protein quantities were compared across groups with ANOVA. Post hoc pair-wise comparisons were adjusted for multiple comparisons.

Results: Compared to the control eyes, the aqueous humor protein concentration was increased approximately tenfold in the Ahmed and Baerveldt eyes and fivefold in the trabeculectomy and Ex-Press eyes. Overall, 718 unique proteins, splice variants, or isoforms were identified. No differences in the protein concentrations were detected between the Baerveldt and Ahmed groups. Likewise, the trabeculectomy and Ex-Press groups were remarkably similar. Therefore, the aqueous shunt groups were pooled, and the trabeculectomy groups were pooled for a three-way comparison with the controls. More than 500 proteins differed significantly in relative abundance (ANOVA p<0.01) among the control, aqueous shunt, and trabeculectomy groups. Functional analyses suggested these alterations in relative protein abundance affected dozens of signaling pathways.

Conclusions: Different glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.

No MeSH data available.


Related in: MedlinePlus

Scatter plots comparing the relative abundance of 718 proteins/isoforms between specified groups were plotted on a log-log scale spanning seven orders of magnitude. The black points represent proteins whose abundance differed significantly between the groups while the aqua points represent proteins whose abundance did not differ significantly between the groups. In some cases, the relative abundance of a given protein differed substantially between the groups, but the difference was not statistically significant because the abundance also varied considerably among the individuals within each group. A: No proteins differed significantly in relative abundance between the Ahmed and Baerveldt groups. B: Only one of the 718 proteins differed significantly between the trabeculectomy and Ex-Press groups. C: The relative abundance of 203 proteins differed significantly between the control group (x-axis) and the pooled aqueous shunt groups (Ahmed and Baerveldt, y-axis). D: The relative abundance of 380 proteins differed significantly between the control group (x-axis) and the pooled trabeculectomy and Ex-Press groups (y-axis).
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f2: Scatter plots comparing the relative abundance of 718 proteins/isoforms between specified groups were plotted on a log-log scale spanning seven orders of magnitude. The black points represent proteins whose abundance differed significantly between the groups while the aqua points represent proteins whose abundance did not differ significantly between the groups. In some cases, the relative abundance of a given protein differed substantially between the groups, but the difference was not statistically significant because the abundance also varied considerably among the individuals within each group. A: No proteins differed significantly in relative abundance between the Ahmed and Baerveldt groups. B: Only one of the 718 proteins differed significantly between the trabeculectomy and Ex-Press groups. C: The relative abundance of 203 proteins differed significantly between the control group (x-axis) and the pooled aqueous shunt groups (Ahmed and Baerveldt, y-axis). D: The relative abundance of 380 proteins differed significantly between the control group (x-axis) and the pooled trabeculectomy and Ex-Press groups (y-axis).

Mentions: We compared the fold-difference in the quantities of each identified protein between groups to determine patterns of protein prevalence. The Ahmed and Baerveldt groups had remarkably similar protein abundance profiles with no significant differences in the relative concentrations of any of the 718 identified proteins/isoforms (Figure 2A). Likewise, the protein abundance profiles were highly similar in the Ex-Press and trabeculectomy groups; only one of the 718 proteins/isoforms differed by a statistically significant amount (Figure 2B).


Distinctive and pervasive alterations in aqueous humor protein composition following different types of glaucoma surgery.

Rosenfeld C, Price MO, Lai X, Witzmann FA, Price FW - Mol. Vis. (2015)

Scatter plots comparing the relative abundance of 718 proteins/isoforms between specified groups were plotted on a log-log scale spanning seven orders of magnitude. The black points represent proteins whose abundance differed significantly between the groups while the aqua points represent proteins whose abundance did not differ significantly between the groups. In some cases, the relative abundance of a given protein differed substantially between the groups, but the difference was not statistically significant because the abundance also varied considerably among the individuals within each group. A: No proteins differed significantly in relative abundance between the Ahmed and Baerveldt groups. B: Only one of the 718 proteins differed significantly between the trabeculectomy and Ex-Press groups. C: The relative abundance of 203 proteins differed significantly between the control group (x-axis) and the pooled aqueous shunt groups (Ahmed and Baerveldt, y-axis). D: The relative abundance of 380 proteins differed significantly between the control group (x-axis) and the pooled trabeculectomy and Ex-Press groups (y-axis).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4548793&req=5

f2: Scatter plots comparing the relative abundance of 718 proteins/isoforms between specified groups were plotted on a log-log scale spanning seven orders of magnitude. The black points represent proteins whose abundance differed significantly between the groups while the aqua points represent proteins whose abundance did not differ significantly between the groups. In some cases, the relative abundance of a given protein differed substantially between the groups, but the difference was not statistically significant because the abundance also varied considerably among the individuals within each group. A: No proteins differed significantly in relative abundance between the Ahmed and Baerveldt groups. B: Only one of the 718 proteins differed significantly between the trabeculectomy and Ex-Press groups. C: The relative abundance of 203 proteins differed significantly between the control group (x-axis) and the pooled aqueous shunt groups (Ahmed and Baerveldt, y-axis). D: The relative abundance of 380 proteins differed significantly between the control group (x-axis) and the pooled trabeculectomy and Ex-Press groups (y-axis).
Mentions: We compared the fold-difference in the quantities of each identified protein between groups to determine patterns of protein prevalence. The Ahmed and Baerveldt groups had remarkably similar protein abundance profiles with no significant differences in the relative concentrations of any of the 718 identified proteins/isoforms (Figure 2A). Likewise, the protein abundance profiles were highly similar in the Ex-Press and trabeculectomy groups; only one of the 718 proteins/isoforms differed by a statistically significant amount (Figure 2B).

Bottom Line: To investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.Relative protein quantities were compared across groups with ANOVA.Different glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: Cornea Research Foundation of America, Indianapolis, IN.

ABSTRACT

Purpose: To investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.

Methods: In this cross-sectional study, aqueous humor samples were prospectively collected from control subjects who underwent routine cataract surgery (n=14) and from patients who had different glaucoma filters: Baerveldt aqueous shunt (n=6), Ahmed aqueous shunt (n=6), trabeculectomy (n=5), and Ex-Press trabeculectomy (n=3). Total protein concentrations were determined with Bradford assay. Tryptic digests were analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Proteins were identified with high confidence using stringent criteria and were quantitatively compared with a label-free platform. Relative protein quantities were compared across groups with ANOVA. Post hoc pair-wise comparisons were adjusted for multiple comparisons.

Results: Compared to the control eyes, the aqueous humor protein concentration was increased approximately tenfold in the Ahmed and Baerveldt eyes and fivefold in the trabeculectomy and Ex-Press eyes. Overall, 718 unique proteins, splice variants, or isoforms were identified. No differences in the protein concentrations were detected between the Baerveldt and Ahmed groups. Likewise, the trabeculectomy and Ex-Press groups were remarkably similar. Therefore, the aqueous shunt groups were pooled, and the trabeculectomy groups were pooled for a three-way comparison with the controls. More than 500 proteins differed significantly in relative abundance (ANOVA p<0.01) among the control, aqueous shunt, and trabeculectomy groups. Functional analyses suggested these alterations in relative protein abundance affected dozens of signaling pathways.

Conclusions: Different glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.

No MeSH data available.


Related in: MedlinePlus