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Distinctive and pervasive alterations in aqueous humor protein composition following different types of glaucoma surgery.

Rosenfeld C, Price MO, Lai X, Witzmann FA, Price FW - Mol. Vis. (2015)

Bottom Line: To investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.Relative protein quantities were compared across groups with ANOVA.Different glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: Cornea Research Foundation of America, Indianapolis, IN.

ABSTRACT

Purpose: To investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.

Methods: In this cross-sectional study, aqueous humor samples were prospectively collected from control subjects who underwent routine cataract surgery (n=14) and from patients who had different glaucoma filters: Baerveldt aqueous shunt (n=6), Ahmed aqueous shunt (n=6), trabeculectomy (n=5), and Ex-Press trabeculectomy (n=3). Total protein concentrations were determined with Bradford assay. Tryptic digests were analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Proteins were identified with high confidence using stringent criteria and were quantitatively compared with a label-free platform. Relative protein quantities were compared across groups with ANOVA. Post hoc pair-wise comparisons were adjusted for multiple comparisons.

Results: Compared to the control eyes, the aqueous humor protein concentration was increased approximately tenfold in the Ahmed and Baerveldt eyes and fivefold in the trabeculectomy and Ex-Press eyes. Overall, 718 unique proteins, splice variants, or isoforms were identified. No differences in the protein concentrations were detected between the Baerveldt and Ahmed groups. Likewise, the trabeculectomy and Ex-Press groups were remarkably similar. Therefore, the aqueous shunt groups were pooled, and the trabeculectomy groups were pooled for a three-way comparison with the controls. More than 500 proteins differed significantly in relative abundance (ANOVA p<0.01) among the control, aqueous shunt, and trabeculectomy groups. Functional analyses suggested these alterations in relative protein abundance affected dozens of signaling pathways.

Conclusions: Different glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.

No MeSH data available.


Related in: MedlinePlus

Total protein concentration in aqueous humor by group. In the box plots, the horizontal lines denote medians, the bottom and top of the boxes represent the 25th and 75th percentiles, respectively, and the vertical lines extend to the minimum and maximum values.
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f1: Total protein concentration in aqueous humor by group. In the box plots, the horizontal lines denote medians, the bottom and top of the boxes represent the 25th and 75th percentiles, respectively, and the vertical lines extend to the minimum and maximum values.

Mentions: The total protein concentrations in aqueous humor differed significantly between the groups (p=0.004); see Figure 1. Post hoc pairwise comparisons showed that the control group differed significantly from both aqueous shunt groups (p<0.01); the median protein content was an order of magnitude higher in the Ahmed and Baerveldt groups (0.91 mg/ml and 1.25 mg/ml, respectively) compared with the control group (0.09 mg/ml). The median protein content in the Ex-Press shunt and trabeculectomy groups (0.54 mg/ml and 0.44 mg/ml, respectively) was five- to sixfold higher than that in the control group (0.09 mg/ml), although the differences did not reach the level of statistical significance.


Distinctive and pervasive alterations in aqueous humor protein composition following different types of glaucoma surgery.

Rosenfeld C, Price MO, Lai X, Witzmann FA, Price FW - Mol. Vis. (2015)

Total protein concentration in aqueous humor by group. In the box plots, the horizontal lines denote medians, the bottom and top of the boxes represent the 25th and 75th percentiles, respectively, and the vertical lines extend to the minimum and maximum values.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4548793&req=5

f1: Total protein concentration in aqueous humor by group. In the box plots, the horizontal lines denote medians, the bottom and top of the boxes represent the 25th and 75th percentiles, respectively, and the vertical lines extend to the minimum and maximum values.
Mentions: The total protein concentrations in aqueous humor differed significantly between the groups (p=0.004); see Figure 1. Post hoc pairwise comparisons showed that the control group differed significantly from both aqueous shunt groups (p<0.01); the median protein content was an order of magnitude higher in the Ahmed and Baerveldt groups (0.91 mg/ml and 1.25 mg/ml, respectively) compared with the control group (0.09 mg/ml). The median protein content in the Ex-Press shunt and trabeculectomy groups (0.54 mg/ml and 0.44 mg/ml, respectively) was five- to sixfold higher than that in the control group (0.09 mg/ml), although the differences did not reach the level of statistical significance.

Bottom Line: To investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.Relative protein quantities were compared across groups with ANOVA.Different glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: Cornea Research Foundation of America, Indianapolis, IN.

ABSTRACT

Purpose: To investigate whether specific glaucoma surgeries are associated with differences in aqueous humor protein concentrations compared to eyes without filters.

Methods: In this cross-sectional study, aqueous humor samples were prospectively collected from control subjects who underwent routine cataract surgery (n=14) and from patients who had different glaucoma filters: Baerveldt aqueous shunt (n=6), Ahmed aqueous shunt (n=6), trabeculectomy (n=5), and Ex-Press trabeculectomy (n=3). Total protein concentrations were determined with Bradford assay. Tryptic digests were analyzed with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Proteins were identified with high confidence using stringent criteria and were quantitatively compared with a label-free platform. Relative protein quantities were compared across groups with ANOVA. Post hoc pair-wise comparisons were adjusted for multiple comparisons.

Results: Compared to the control eyes, the aqueous humor protein concentration was increased approximately tenfold in the Ahmed and Baerveldt eyes and fivefold in the trabeculectomy and Ex-Press eyes. Overall, 718 unique proteins, splice variants, or isoforms were identified. No differences in the protein concentrations were detected between the Baerveldt and Ahmed groups. Likewise, the trabeculectomy and Ex-Press groups were remarkably similar. Therefore, the aqueous shunt groups were pooled, and the trabeculectomy groups were pooled for a three-way comparison with the controls. More than 500 proteins differed significantly in relative abundance (ANOVA p<0.01) among the control, aqueous shunt, and trabeculectomy groups. Functional analyses suggested these alterations in relative protein abundance affected dozens of signaling pathways.

Conclusions: Different glaucoma surgical procedures were associated with marked increases in the aqueous humor protein concentration and distinctive changes in the relative abundance of numerous proteins involved in multiple signaling pathways.

No MeSH data available.


Related in: MedlinePlus