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MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27.

Ren YQ, Fu F, Han J - Med. Sci. Monit. (2015)

Bottom Line: CDC-27 is a direct target of miR-27a and its downregulation conferred increased radioresistance of the cells.The miR-27a-CDC27 axis might play an important role in modulating response to radiotherapy in TNBC cells.Testing miR-27a expression might be a useful way to identify a subgroup of patients who will benefit from an IR-based therapeutic approach.

View Article: PubMed Central - PubMed

Affiliation: Clinical Laboratory, The Central Hospital of Yishui, Linyi, Shandong, China (mainland).

ABSTRACT

Background: MiR-27a is significantly overexpressed in triple-negative breast cancer (TNBC). However, the exact biological function of MiR-27a in TNBC is not fully understood. In this study, we verified miR-27a expression in TNBC cells and explored how its overexpression modulates radiosensitivity of the cells.

Material/methods: qRT-PCR analysis was performed to study miR-27a expression in TNBC lines MDA-MB-435 and MDA-MB-231 and in normal human breast epithelial cell line MCF10A. Dual luciferase assay was performed to verify a putative downstream target of miR-27a, CDC27. CCK-8 assay was used to assess the influence of miR-27a-CDC27 axis on cell proliferation under irradiation (IR) treatment.

Results: We confirmed significantly higher miR-27a expression in 2 TNBC cell lines--MDA-MB-435 and MDA-MB-231--than in human breast epithelial cell line MCF10A. miR-27a could modulate proliferation and radiosensitivity of TNBC cells. CDC-27 is a direct target of miR-27a and its downregulation conferred increased radioresistance of the cells.

Conclusions: The miR-27a-CDC27 axis might play an important role in modulating response to radiotherapy in TNBC cells. Testing miR-27a expression might be a useful way to identify a subgroup of patients who will benefit from an IR-based therapeutic approach.

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Related in: MedlinePlus

MiR-27a is significantly increased and CDC27 expression is significantly decreased in TNBC cells. (A) qRT-PCR analysis of miR-27a expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. (B) qRT-PCR analysis of CDC27 mRNA expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. (C) Western blot analysis of CDC27 expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. Data are shown as mean±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.
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f1-medscimonit-21-1297: MiR-27a is significantly increased and CDC27 expression is significantly decreased in TNBC cells. (A) qRT-PCR analysis of miR-27a expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. (B) qRT-PCR analysis of CDC27 mRNA expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. (C) Western blot analysis of CDC27 expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. Data are shown as mean±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.

Mentions: To assess the role of miR-27a in TNBC, TNBC cell line MDA-MB-435 and MDA-MB-231 and normal human breast epithelial cell line MCF10A were used. qRT-PCR analysis showed that miR-27a expression was significantly higher in MDA-MB-435 and MDA-MB-231 cells than in MCF10A cells (Figure 1A). Through preliminary searching and comparison, we identified CDC27 as a highly possible downstream target of miR-27a. Therefore, we also explored the expression of this protein in the cells. In contrast, CDC27 mRNA and protein expression was significantly lower in the 2 cancer cell lines than in the normal cell line (Figure 1B, 1C). These results suggested that miR-27a is significantly associated with TNBC cells, while its expression is negatively correlated with CDC27 expression.


MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27.

Ren YQ, Fu F, Han J - Med. Sci. Monit. (2015)

MiR-27a is significantly increased and CDC27 expression is significantly decreased in TNBC cells. (A) qRT-PCR analysis of miR-27a expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. (B) qRT-PCR analysis of CDC27 mRNA expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. (C) Western blot analysis of CDC27 expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. Data are shown as mean±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4548742&req=5

f1-medscimonit-21-1297: MiR-27a is significantly increased and CDC27 expression is significantly decreased in TNBC cells. (A) qRT-PCR analysis of miR-27a expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. (B) qRT-PCR analysis of CDC27 mRNA expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. (C) Western blot analysis of CDC27 expression in MDA-MB-435, MDA-MB-231, and MCF10A cell lines. Data are shown as mean±S.D by 3 independent experiments. * P<0.05, ** P<0.01, *** P<0.001.
Mentions: To assess the role of miR-27a in TNBC, TNBC cell line MDA-MB-435 and MDA-MB-231 and normal human breast epithelial cell line MCF10A were used. qRT-PCR analysis showed that miR-27a expression was significantly higher in MDA-MB-435 and MDA-MB-231 cells than in MCF10A cells (Figure 1A). Through preliminary searching and comparison, we identified CDC27 as a highly possible downstream target of miR-27a. Therefore, we also explored the expression of this protein in the cells. In contrast, CDC27 mRNA and protein expression was significantly lower in the 2 cancer cell lines than in the normal cell line (Figure 1B, 1C). These results suggested that miR-27a is significantly associated with TNBC cells, while its expression is negatively correlated with CDC27 expression.

Bottom Line: CDC-27 is a direct target of miR-27a and its downregulation conferred increased radioresistance of the cells.The miR-27a-CDC27 axis might play an important role in modulating response to radiotherapy in TNBC cells.Testing miR-27a expression might be a useful way to identify a subgroup of patients who will benefit from an IR-based therapeutic approach.

View Article: PubMed Central - PubMed

Affiliation: Clinical Laboratory, The Central Hospital of Yishui, Linyi, Shandong, China (mainland).

ABSTRACT

Background: MiR-27a is significantly overexpressed in triple-negative breast cancer (TNBC). However, the exact biological function of MiR-27a in TNBC is not fully understood. In this study, we verified miR-27a expression in TNBC cells and explored how its overexpression modulates radiosensitivity of the cells.

Material/methods: qRT-PCR analysis was performed to study miR-27a expression in TNBC lines MDA-MB-435 and MDA-MB-231 and in normal human breast epithelial cell line MCF10A. Dual luciferase assay was performed to verify a putative downstream target of miR-27a, CDC27. CCK-8 assay was used to assess the influence of miR-27a-CDC27 axis on cell proliferation under irradiation (IR) treatment.

Results: We confirmed significantly higher miR-27a expression in 2 TNBC cell lines--MDA-MB-435 and MDA-MB-231--than in human breast epithelial cell line MCF10A. miR-27a could modulate proliferation and radiosensitivity of TNBC cells. CDC-27 is a direct target of miR-27a and its downregulation conferred increased radioresistance of the cells.

Conclusions: The miR-27a-CDC27 axis might play an important role in modulating response to radiotherapy in TNBC cells. Testing miR-27a expression might be a useful way to identify a subgroup of patients who will benefit from an IR-based therapeutic approach.

Show MeSH
Related in: MedlinePlus