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Proteomic profiling of aging in glomeruli of mice by using two-dimensional differential gel electrophoresis.

Liu X, Fan Q, Yang G, Wang L - Med. Sci. Monit. (2015)

Bottom Line: Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice.Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging.In this study we found that aging altered the expression of ATP synthase subunit beta.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).

ABSTRACT

Background: Glomerular proteins were analyzed by proteomics to screen proteins participating in maturation of glomeruli before senescence and to find key proteins involved in the aging process.

Material and methods: Glomeruli of C57BL/6 mice at 8 and 20 weeks were separated by kidney perfusion. Proteomic profiles of glomeruli were investigated by using two-dimensional differential gel electrophoresis and MALDI-TOF mass spectrometry.

Results: We identified 22 differentially expressed proteins. Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice. Out of these 22 proteins, 18% take part in protein transport, protein targeting, and proteolysis; 5% in glycolysis; 14% in transcription; 9% in electron transport; 9% were chaperones; and 9% were hydrolases.

Conclusions: Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging. In this study we found that aging altered the expression of ATP synthase subunit beta. Further studies on this protein might help to understand the mechanism of renal aging.

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Related in: MedlinePlus

Two-dimensional electrophoresis image of differentially expressed glomerular proteins during maturation. Glomerular proteins were separated by 2-dimensional gel electrophoresis (2-DE). The gel was stained with Deep Purple dye. Twenty-two differentially expressed proteins were labeled on the map. Marked spots represent differentially expressed proteins between young (8 weeks) and mature (20 weeks) C57BL/6 mice. The marked spot numbers refer to “Master No.” in Tables 2 and 3.
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f2-medscimonit-21-419: Two-dimensional electrophoresis image of differentially expressed glomerular proteins during maturation. Glomerular proteins were separated by 2-dimensional gel electrophoresis (2-DE). The gel was stained with Deep Purple dye. Twenty-two differentially expressed proteins were labeled on the map. Marked spots represent differentially expressed proteins between young (8 weeks) and mature (20 weeks) C57BL/6 mice. The marked spot numbers refer to “Master No.” in Tables 2 and 3.

Mentions: After 2-dimensional differential gel electrophoresis, 2387±129 glomerular protein spots were detected. By using DeCyder™ 2-D Differential Analysis Software, 151 differentially expressed protein spots were extracted for further mass spectrometry analysis. By the individual analysis of MALDI-TOF mass spectrometry and peptide mass fingerprinting, 22 differentially expressed glomerular proteins were identified (Figure 2). Among these proteins, 3 proteins were significantly up-regulated and 19 were significantly down-regulated in mature mice (Tables 2 and 3). The distribution of these proteins in different cellular functions are as follows: protein transport, protein targeting, and proteolysis, 18%; glycolysis, 5%; transcription, 14%; electron transport, 9%; chaperones, 9%; and hydrolase, 9%. Among the glomerular differential proteins attained by us, 82% were in accord with the results attained by Tryqqvason et al. [11] (Tables 2 and 3).


Proteomic profiling of aging in glomeruli of mice by using two-dimensional differential gel electrophoresis.

Liu X, Fan Q, Yang G, Wang L - Med. Sci. Monit. (2015)

Two-dimensional electrophoresis image of differentially expressed glomerular proteins during maturation. Glomerular proteins were separated by 2-dimensional gel electrophoresis (2-DE). The gel was stained with Deep Purple dye. Twenty-two differentially expressed proteins were labeled on the map. Marked spots represent differentially expressed proteins between young (8 weeks) and mature (20 weeks) C57BL/6 mice. The marked spot numbers refer to “Master No.” in Tables 2 and 3.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4548697&req=5

f2-medscimonit-21-419: Two-dimensional electrophoresis image of differentially expressed glomerular proteins during maturation. Glomerular proteins were separated by 2-dimensional gel electrophoresis (2-DE). The gel was stained with Deep Purple dye. Twenty-two differentially expressed proteins were labeled on the map. Marked spots represent differentially expressed proteins between young (8 weeks) and mature (20 weeks) C57BL/6 mice. The marked spot numbers refer to “Master No.” in Tables 2 and 3.
Mentions: After 2-dimensional differential gel electrophoresis, 2387±129 glomerular protein spots were detected. By using DeCyder™ 2-D Differential Analysis Software, 151 differentially expressed protein spots were extracted for further mass spectrometry analysis. By the individual analysis of MALDI-TOF mass spectrometry and peptide mass fingerprinting, 22 differentially expressed glomerular proteins were identified (Figure 2). Among these proteins, 3 proteins were significantly up-regulated and 19 were significantly down-regulated in mature mice (Tables 2 and 3). The distribution of these proteins in different cellular functions are as follows: protein transport, protein targeting, and proteolysis, 18%; glycolysis, 5%; transcription, 14%; electron transport, 9%; chaperones, 9%; and hydrolase, 9%. Among the glomerular differential proteins attained by us, 82% were in accord with the results attained by Tryqqvason et al. [11] (Tables 2 and 3).

Bottom Line: Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice.Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging.In this study we found that aging altered the expression of ATP synthase subunit beta.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).

ABSTRACT

Background: Glomerular proteins were analyzed by proteomics to screen proteins participating in maturation of glomeruli before senescence and to find key proteins involved in the aging process.

Material and methods: Glomeruli of C57BL/6 mice at 8 and 20 weeks were separated by kidney perfusion. Proteomic profiles of glomeruli were investigated by using two-dimensional differential gel electrophoresis and MALDI-TOF mass spectrometry.

Results: We identified 22 differentially expressed proteins. Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice. Out of these 22 proteins, 18% take part in protein transport, protein targeting, and proteolysis; 5% in glycolysis; 14% in transcription; 9% in electron transport; 9% were chaperones; and 9% were hydrolases.

Conclusions: Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging. In this study we found that aging altered the expression of ATP synthase subunit beta. Further studies on this protein might help to understand the mechanism of renal aging.

Show MeSH
Related in: MedlinePlus