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Proteomic profiling of aging in glomeruli of mice by using two-dimensional differential gel electrophoresis.

Liu X, Fan Q, Yang G, Wang L - Med. Sci. Monit. (2015)

Bottom Line: Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice.In this study we found that aging altered the expression of ATP synthase subunit beta.Further studies on this protein might help to understand the mechanism of renal aging.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).

ABSTRACT

Background: Glomerular proteins were analyzed by proteomics to screen proteins participating in maturation of glomeruli before senescence and to find key proteins involved in the aging process.

Material and methods: Glomeruli of C57BL/6 mice at 8 and 20 weeks were separated by kidney perfusion. Proteomic profiles of glomeruli were investigated by using two-dimensional differential gel electrophoresis and MALDI-TOF mass spectrometry.

Results: We identified 22 differentially expressed proteins. Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice. Out of these 22 proteins, 18% take part in protein transport, protein targeting, and proteolysis; 5% in glycolysis; 14% in transcription; 9% in electron transport; 9% were chaperones; and 9% were hydrolases.

Conclusions: Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging. In this study we found that aging altered the expression of ATP synthase subunit beta. Further studies on this protein might help to understand the mechanism of renal aging.

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Related in: MedlinePlus

Representative age ranges for mature life history stages in C57BL/6J mice. (Fox JG, Barthold SW, Davisson MT et al.: The mouse in biomedical research, 2nd edition: Diseases, Elsevier, 2007; p. 645)
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f1-medscimonit-21-419: Representative age ranges for mature life history stages in C57BL/6J mice. (Fox JG, Barthold SW, Davisson MT et al.: The mouse in biomedical research, 2nd edition: Diseases, Elsevier, 2007; p. 645)

Mentions: The physiological mechanisms of renal maturation and lack of renal reserve, which eventually leads to senescence, remain largely unknown. To understand the molecular basis of benign development of glomeruli, and to investigate proteins participating in the pre- and post-maturation periods, we analyzed the expression of glomerular proteins in C57BL/6 mice, between young (8 weeks) and mature (20 weeks) stages, which closely represents the benign development of human kidneys (Figure 1). By using the Dynabeads perfusion developed by Takemoto et al. [9], we have successfully isolated glomeruli with high purity [10]. By using two-dimensional differential gel electrophoresis (2D-DIGE), glomerular proteins were separated. With the aid of DeCyderâ„¢ 2-D Differential Analysis software, significantly differential protein spots due to aging were obtained. Subsequently, differential protein spots have been identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.


Proteomic profiling of aging in glomeruli of mice by using two-dimensional differential gel electrophoresis.

Liu X, Fan Q, Yang G, Wang L - Med. Sci. Monit. (2015)

Representative age ranges for mature life history stages in C57BL/6J mice. (Fox JG, Barthold SW, Davisson MT et al.: The mouse in biomedical research, 2nd edition: Diseases, Elsevier, 2007; p. 645)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4548697&req=5

f1-medscimonit-21-419: Representative age ranges for mature life history stages in C57BL/6J mice. (Fox JG, Barthold SW, Davisson MT et al.: The mouse in biomedical research, 2nd edition: Diseases, Elsevier, 2007; p. 645)
Mentions: The physiological mechanisms of renal maturation and lack of renal reserve, which eventually leads to senescence, remain largely unknown. To understand the molecular basis of benign development of glomeruli, and to investigate proteins participating in the pre- and post-maturation periods, we analyzed the expression of glomerular proteins in C57BL/6 mice, between young (8 weeks) and mature (20 weeks) stages, which closely represents the benign development of human kidneys (Figure 1). By using the Dynabeads perfusion developed by Takemoto et al. [9], we have successfully isolated glomeruli with high purity [10]. By using two-dimensional differential gel electrophoresis (2D-DIGE), glomerular proteins were separated. With the aid of DeCyderâ„¢ 2-D Differential Analysis software, significantly differential protein spots due to aging were obtained. Subsequently, differential protein spots have been identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry.

Bottom Line: Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice.In this study we found that aging altered the expression of ATP synthase subunit beta.Further studies on this protein might help to understand the mechanism of renal aging.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland).

ABSTRACT

Background: Glomerular proteins were analyzed by proteomics to screen proteins participating in maturation of glomeruli before senescence and to find key proteins involved in the aging process.

Material and methods: Glomeruli of C57BL/6 mice at 8 and 20 weeks were separated by kidney perfusion. Proteomic profiles of glomeruli were investigated by using two-dimensional differential gel electrophoresis and MALDI-TOF mass spectrometry.

Results: We identified 22 differentially expressed proteins. Among them, 3 proteins were significantly up-regulated and 19 proteins were significantly down-regulated in mature mice. Out of these 22 proteins, 18% take part in protein transport, protein targeting, and proteolysis; 5% in glycolysis; 14% in transcription; 9% in electron transport; 9% were chaperones; and 9% were hydrolases.

Conclusions: Our results provide insights into the glomerular differentially expressed proteins correlated with renal aging. In this study we found that aging altered the expression of ATP synthase subunit beta. Further studies on this protein might help to understand the mechanism of renal aging.

Show MeSH
Related in: MedlinePlus