Limits...
Analysis of the pathogenic potential of nosocomial Pseudomonas putida strains.

Fernández M, Porcel M, de la Torre J, Molina-Henares MA, Daddaoua A, Llamas MA, Roca A, Carriel V, Garzón I, Ramos JL, Alaminos M, Duque E - Front Microbiol (2015)

Bottom Line: In this study we describe the multilocus sequence typing of four P. putida strains (HB13667, HB8234, HB4184, and HB3267) isolated from in-patients at the Besançon Hospital (France).The four isolates (in particular HB3267) were resistant to a number of antibiotics.The putative evolution of these strains in medical environments is discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas Granada, Spain ; Bio-Iliberis R&D Granada, Spain.

ABSTRACT
Pseudomonas putida strains are ubiquitous in soil and water but have also been reported as opportunistic human pathogens capable of causing nosocomial infections. In this study we describe the multilocus sequence typing of four P. putida strains (HB13667, HB8234, HB4184, and HB3267) isolated from in-patients at the Besançon Hospital (France). The four isolates (in particular HB3267) were resistant to a number of antibiotics. The pathogenicity and virulence potential of the strains was tested ex vivo and in vivo using different biological models: human tissue culture, mammalian tissues, and insect larvae. Our results showed a significant variability in the ability of the four strains to damage the host; HB13667 did not exhibit any pathogenic traits, HB4184 caused damage only ex vivo in human tissue cultures, and HB8234 had a deleterious effect in tissue culture and in vivo on rat skin, but not in insect larvae. Interestingly, strain HB3267 caused damage in all the model systems studied. The putative evolution of these strains in medical environments is discussed.

No MeSH data available.


Related in: MedlinePlus

Analysis of the integrity of epithelial cell-cell junctions as determined by immunohistofluorescence for ZO-2 (upper) and desmoplakin (lower) in human skin samples incubated with the different P. putida strains (HB13667, HB8234, HB4184, and HB3267) and control non-inoculated skin (CTR). Positive signal is labelled in green and cell nuclei have been counterstained in red.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4548156&req=5

Figure 5: Analysis of the integrity of epithelial cell-cell junctions as determined by immunohistofluorescence for ZO-2 (upper) and desmoplakin (lower) in human skin samples incubated with the different P. putida strains (HB13667, HB8234, HB4184, and HB3267) and control non-inoculated skin (CTR). Positive signal is labelled in green and cell nuclei have been counterstained in red.

Mentions: When two specific proteins with a key role in cell-cell attachment (ZO-2 and desmoplakin) were analyzed by immunofluorescence, we found that the signal intensity of both components of the cell-cell junction complex were reduced after exposer to the different P. putida strains (Figure 5). Incubation of the human skin tissues in the presence of HB13667, HB8234, and HB3267 significantly reduced the expression of ZO-2, a key component of the tight junctions which maintain the barrier function of the human skin. We also found that desmoplakin, a protein with an important role in cell-cell attachment via adherent junctions, was altered in skin incubated in all strains; again this was especially noticeable in the presence of HB3267.


Analysis of the pathogenic potential of nosocomial Pseudomonas putida strains.

Fernández M, Porcel M, de la Torre J, Molina-Henares MA, Daddaoua A, Llamas MA, Roca A, Carriel V, Garzón I, Ramos JL, Alaminos M, Duque E - Front Microbiol (2015)

Analysis of the integrity of epithelial cell-cell junctions as determined by immunohistofluorescence for ZO-2 (upper) and desmoplakin (lower) in human skin samples incubated with the different P. putida strains (HB13667, HB8234, HB4184, and HB3267) and control non-inoculated skin (CTR). Positive signal is labelled in green and cell nuclei have been counterstained in red.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4548156&req=5

Figure 5: Analysis of the integrity of epithelial cell-cell junctions as determined by immunohistofluorescence for ZO-2 (upper) and desmoplakin (lower) in human skin samples incubated with the different P. putida strains (HB13667, HB8234, HB4184, and HB3267) and control non-inoculated skin (CTR). Positive signal is labelled in green and cell nuclei have been counterstained in red.
Mentions: When two specific proteins with a key role in cell-cell attachment (ZO-2 and desmoplakin) were analyzed by immunofluorescence, we found that the signal intensity of both components of the cell-cell junction complex were reduced after exposer to the different P. putida strains (Figure 5). Incubation of the human skin tissues in the presence of HB13667, HB8234, and HB3267 significantly reduced the expression of ZO-2, a key component of the tight junctions which maintain the barrier function of the human skin. We also found that desmoplakin, a protein with an important role in cell-cell attachment via adherent junctions, was altered in skin incubated in all strains; again this was especially noticeable in the presence of HB3267.

Bottom Line: In this study we describe the multilocus sequence typing of four P. putida strains (HB13667, HB8234, HB4184, and HB3267) isolated from in-patients at the Besançon Hospital (France).The four isolates (in particular HB3267) were resistant to a number of antibiotics.The putative evolution of these strains in medical environments is discussed.

View Article: PubMed Central - PubMed

Affiliation: Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas Granada, Spain ; Bio-Iliberis R&D Granada, Spain.

ABSTRACT
Pseudomonas putida strains are ubiquitous in soil and water but have also been reported as opportunistic human pathogens capable of causing nosocomial infections. In this study we describe the multilocus sequence typing of four P. putida strains (HB13667, HB8234, HB4184, and HB3267) isolated from in-patients at the Besançon Hospital (France). The four isolates (in particular HB3267) were resistant to a number of antibiotics. The pathogenicity and virulence potential of the strains was tested ex vivo and in vivo using different biological models: human tissue culture, mammalian tissues, and insect larvae. Our results showed a significant variability in the ability of the four strains to damage the host; HB13667 did not exhibit any pathogenic traits, HB4184 caused damage only ex vivo in human tissue cultures, and HB8234 had a deleterious effect in tissue culture and in vivo on rat skin, but not in insect larvae. Interestingly, strain HB3267 caused damage in all the model systems studied. The putative evolution of these strains in medical environments is discussed.

No MeSH data available.


Related in: MedlinePlus