Limits...
The roles of connective tissue growth factor in the development of anastomotic esophageal strictures.

Zhao H, Zhao L, Zhou Z, Wu Y - Arch Med Sci (2015)

Bottom Line: The aim of this study was to investigate the roles of connective tissue growth factor (CTGF) in the development of anastomotic strictures after surgical repair of the esophagus.Tissues collected from the patients were divided into three groups based on the results of endoscopy and clinical grading.Inhibition of CTGF protein or mRNA expression may be a distinctive and effective therapy for the treatment of postoperative anastomotic strictures.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, the 101 Hospital of Chinese People's Liberation Army, Wuxi, Jiangsu Province, China.

ABSTRACT

Introduction: The aim of this study was to investigate the roles of connective tissue growth factor (CTGF) in the development of anastomotic strictures after surgical repair of the esophagus.

Material and methods: Tissues collected from the patients were divided into three groups based on the results of endoscopy and clinical grading. Patients without dysphagia after esophagectomy were used as the control population. The protein levels of CTGF, TGF-β1, Smad2, and Smad4 were determined by immunohistochemistry (IHC) and western blot analyses, while the mRNA levels of the two growth factors were evaluated by real-time polymerase chain reaction.

Results: Compared with the control group, significantly increased (p < 0.01) levels of CTGF and TGF-β1 protein were observed in the anastomotic stenosis (AS) group, and levels of the two proteins detected by the IHC and western blot analyses were also significantly increased with the increasing severity of stenosis (p < 0.05). The mRNA levels of CTGF and TGF-β1 in the tissues collected from the patients with stenosis were significantly up-regulated (p < 0.05) as compared with those from the control group. In addition, the levels of Smad2 and Smad4 protein were also significantly increased (p < 0.05) with the increasing severity of stenosis, and the protein levels were positively correlated with the levels of CTGF (r = 0.59, p < 0.05) and TGF-β1 (r = 0.63, p < 0.05).

Conclusions: Inhibition of CTGF protein or mRNA expression may be a distinctive and effective therapy for the treatment of postoperative anastomotic strictures.

No MeSH data available.


Related in: MedlinePlus

Relative expression of TGF-β1 and CTGF mRNA compared with the GAPDH housekeeping gene. The anastomotic strictures group showed significant upregulation in relative mRNA levels of TGF-β1 and CTGF as compared with those of the control group (p < 0.05). The mRNA levels of TGF-β1 and CTGF increased with the severity of strictures (p < 0.05)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4548024&req=5

Figure 0006: Relative expression of TGF-β1 and CTGF mRNA compared with the GAPDH housekeeping gene. The anastomotic strictures group showed significant upregulation in relative mRNA levels of TGF-β1 and CTGF as compared with those of the control group (p < 0.05). The mRNA levels of TGF-β1 and CTGF increased with the severity of strictures (p < 0.05)

Mentions: The mRNA levels of TGF-β1 and CTGF were determined by RT-PCR analysis. The mRNA levels detected by RT-PCR are shown in Figure 5 and levels of TGF-β1 and CTGF mRNA were determined by densitometric analysis (Figure 6). As shown in Figures 5 and 6, levels of TGF-β1 and CTGF mRNA in the AS group were significantly up-regulated (p < 0.05) compared with the control group, and the mRNA levels significantly increased (p < 0.05) with the increasing severity of AS.


The roles of connective tissue growth factor in the development of anastomotic esophageal strictures.

Zhao H, Zhao L, Zhou Z, Wu Y - Arch Med Sci (2015)

Relative expression of TGF-β1 and CTGF mRNA compared with the GAPDH housekeeping gene. The anastomotic strictures group showed significant upregulation in relative mRNA levels of TGF-β1 and CTGF as compared with those of the control group (p < 0.05). The mRNA levels of TGF-β1 and CTGF increased with the severity of strictures (p < 0.05)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4548024&req=5

Figure 0006: Relative expression of TGF-β1 and CTGF mRNA compared with the GAPDH housekeeping gene. The anastomotic strictures group showed significant upregulation in relative mRNA levels of TGF-β1 and CTGF as compared with those of the control group (p < 0.05). The mRNA levels of TGF-β1 and CTGF increased with the severity of strictures (p < 0.05)
Mentions: The mRNA levels of TGF-β1 and CTGF were determined by RT-PCR analysis. The mRNA levels detected by RT-PCR are shown in Figure 5 and levels of TGF-β1 and CTGF mRNA were determined by densitometric analysis (Figure 6). As shown in Figures 5 and 6, levels of TGF-β1 and CTGF mRNA in the AS group were significantly up-regulated (p < 0.05) compared with the control group, and the mRNA levels significantly increased (p < 0.05) with the increasing severity of AS.

Bottom Line: The aim of this study was to investigate the roles of connective tissue growth factor (CTGF) in the development of anastomotic strictures after surgical repair of the esophagus.Tissues collected from the patients were divided into three groups based on the results of endoscopy and clinical grading.Inhibition of CTGF protein or mRNA expression may be a distinctive and effective therapy for the treatment of postoperative anastomotic strictures.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, the 101 Hospital of Chinese People's Liberation Army, Wuxi, Jiangsu Province, China.

ABSTRACT

Introduction: The aim of this study was to investigate the roles of connective tissue growth factor (CTGF) in the development of anastomotic strictures after surgical repair of the esophagus.

Material and methods: Tissues collected from the patients were divided into three groups based on the results of endoscopy and clinical grading. Patients without dysphagia after esophagectomy were used as the control population. The protein levels of CTGF, TGF-β1, Smad2, and Smad4 were determined by immunohistochemistry (IHC) and western blot analyses, while the mRNA levels of the two growth factors were evaluated by real-time polymerase chain reaction.

Results: Compared with the control group, significantly increased (p < 0.01) levels of CTGF and TGF-β1 protein were observed in the anastomotic stenosis (AS) group, and levels of the two proteins detected by the IHC and western blot analyses were also significantly increased with the increasing severity of stenosis (p < 0.05). The mRNA levels of CTGF and TGF-β1 in the tissues collected from the patients with stenosis were significantly up-regulated (p < 0.05) as compared with those from the control group. In addition, the levels of Smad2 and Smad4 protein were also significantly increased (p < 0.05) with the increasing severity of stenosis, and the protein levels were positively correlated with the levels of CTGF (r = 0.59, p < 0.05) and TGF-β1 (r = 0.63, p < 0.05).

Conclusions: Inhibition of CTGF protein or mRNA expression may be a distinctive and effective therapy for the treatment of postoperative anastomotic strictures.

No MeSH data available.


Related in: MedlinePlus