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Ultrastructural analysis between fetal and adult wound healing process of marsupial opossum skin.

Matsuno K, Ihara S - Exp. Anim. (2015)

Bottom Line: The quantity of extracellular matrix of the dermis increased thereafter, and several types of cells were found in the dermis.To examine the wound healing, we used material from a 1 day-old newborn baby, and from another 15 days after birth, and compared the wound healing style morphologically.Differences in the constitution of cells and fine structures of the skin were observed, it was obviously suggested that change in the wound healing style from fetal-type to adult-type occurred between 1 to 15 days after birth.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agriculture, Tottori University, Nishikawatsu 1060, Matsue, Shimane 690-8504, Japan.

ABSTRACT
The opossum delivers a newborn baby equivalent to tremature fetus state by postpregnancy. The peculiarity is advantageous for studies of fetus, because operations to take out fetus from the uterus of a mother are not necessary. When mammalian skin is wounded by full-thickness excision, fetal and adult wound healing processes differ. Fetal-type wound healing does not leave a scar. However, studies of how the fetal wound healing process differs in detail from the adult type are not advanced. We first observed the normal skin development of the gray short-tailed opossum (Monodelphis domestica) using an electron microscope. As for normal skin, an epidermis became multi-layered, and thickened from birth through to 7 days after birth. The quantity of extracellular matrix of the dermis increased thereafter, and several types of cells were found in the dermis. To examine the wound healing, we used material from a 1 day-old newborn baby, and from another 15 days after birth, and compared the wound healing style morphologically. Differences in the constitution of cells and fine structures of the skin were observed, it was obviously suggested that change in the wound healing style from fetal-type to adult-type occurred between 1 to 15 days after birth.

No MeSH data available.


Related in: MedlinePlus

A. Electron micrograph of a scab and its vicinity in a 15-day old baby 1 dayafter injury. The cell component is not confirmed, and vesicles are distributedover the scab. Cells contacting the scab are of unknown origin. SCAB; scab. Scalebar 2µm. B. Electron micrograph of the wall of a wound in a15-day old baby, 1 day after injury. Cells which seem to be extending from thewound area are distributed at the wound wall. The place in the upper region wheresquamous cells are not distributed is the edge of the wound (arrow). Scale bar 2µm. C. Electron micrograph of a scab and an epidermis in a 15day-old baby, 3 days after injury. Keratinocyte cells or layers are not observed,but the epidermis covers the wound. SCAB; scab Scale bar 2 µm. D.Electron micrograph of a dermis in a 15 day-old baby, 3 days after injury. Bundlesof fibers and fibroblasts are densely distributed, and the intercellular space issmall. OF; oval or polygonal fibroblast, SF; slim fibroblast. Scale bar 2µm. E. Electron micrograph of an epidermis in a 15 day-oldbaby, 6 days after injury. Large squamous cells are arranged in layers. Basalcells are clearly distinguishable from squamous cells. BC; basal cell, SC;squamous cell. Scale bar 10 µm. F. Electron micrograph of adermis in a 15 day-old baby, 6 days after injury. Oval or polygonal fibroblastsare mainly distributed in the upper part of the dermis. OF; oval or polygonal typefibroblast, SF; slim type fibroblast. Scale bar 10 µm. G.Electron micrograph of a fibroblast in a 15 day-old baby, 6 days after injury.Abundant endoplasmic reticula are distributed in the cytoplasm. Collagen fibersare gathered into bundles in the intercellular space. Scale bar 1µm. H. Electron micrograph of a granulation-like tissue in a 15day-old baby, 6 days after injury. The tissue was observed in a dermis and showeda compact packing group of many fibroblasts and some macrophage in it. MF;macrophage Scale bar 2 µm.
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fig_005: A. Electron micrograph of a scab and its vicinity in a 15-day old baby 1 dayafter injury. The cell component is not confirmed, and vesicles are distributedover the scab. Cells contacting the scab are of unknown origin. SCAB; scab. Scalebar 2µm. B. Electron micrograph of the wall of a wound in a15-day old baby, 1 day after injury. Cells which seem to be extending from thewound area are distributed at the wound wall. The place in the upper region wheresquamous cells are not distributed is the edge of the wound (arrow). Scale bar 2µm. C. Electron micrograph of a scab and an epidermis in a 15day-old baby, 3 days after injury. Keratinocyte cells or layers are not observed,but the epidermis covers the wound. SCAB; scab Scale bar 2 µm. D.Electron micrograph of a dermis in a 15 day-old baby, 3 days after injury. Bundlesof fibers and fibroblasts are densely distributed, and the intercellular space issmall. OF; oval or polygonal fibroblast, SF; slim fibroblast. Scale bar 2µm. E. Electron micrograph of an epidermis in a 15 day-oldbaby, 6 days after injury. Large squamous cells are arranged in layers. Basalcells are clearly distinguishable from squamous cells. BC; basal cell, SC;squamous cell. Scale bar 10 µm. F. Electron micrograph of adermis in a 15 day-old baby, 6 days after injury. Oval or polygonal fibroblastsare mainly distributed in the upper part of the dermis. OF; oval or polygonal typefibroblast, SF; slim type fibroblast. Scale bar 10 µm. G.Electron micrograph of a fibroblast in a 15 day-old baby, 6 days after injury.Abundant endoplasmic reticula are distributed in the cytoplasm. Collagen fibersare gathered into bundles in the intercellular space. Scale bar 1µm. H. Electron micrograph of a granulation-like tissue in a 15day-old baby, 6 days after injury. The tissue was observed in a dermis and showeda compact packing group of many fibroblasts and some macrophage in it. MF;macrophage Scale bar 2 µm.

Mentions: The wounded area was filled with scab, and vesicles and a homogeneous framework wasdistributed beneath the scab. The origin of the vesicles and the framework was unknown,but both were presumably secreted by cells neighboring the wall of the wound. Theframework contained abundant small vesicles and granules (Fig. 5AFig. 5.


Ultrastructural analysis between fetal and adult wound healing process of marsupial opossum skin.

Matsuno K, Ihara S - Exp. Anim. (2015)

A. Electron micrograph of a scab and its vicinity in a 15-day old baby 1 dayafter injury. The cell component is not confirmed, and vesicles are distributedover the scab. Cells contacting the scab are of unknown origin. SCAB; scab. Scalebar 2µm. B. Electron micrograph of the wall of a wound in a15-day old baby, 1 day after injury. Cells which seem to be extending from thewound area are distributed at the wound wall. The place in the upper region wheresquamous cells are not distributed is the edge of the wound (arrow). Scale bar 2µm. C. Electron micrograph of a scab and an epidermis in a 15day-old baby, 3 days after injury. Keratinocyte cells or layers are not observed,but the epidermis covers the wound. SCAB; scab Scale bar 2 µm. D.Electron micrograph of a dermis in a 15 day-old baby, 3 days after injury. Bundlesof fibers and fibroblasts are densely distributed, and the intercellular space issmall. OF; oval or polygonal fibroblast, SF; slim fibroblast. Scale bar 2µm. E. Electron micrograph of an epidermis in a 15 day-oldbaby, 6 days after injury. Large squamous cells are arranged in layers. Basalcells are clearly distinguishable from squamous cells. BC; basal cell, SC;squamous cell. Scale bar 10 µm. F. Electron micrograph of adermis in a 15 day-old baby, 6 days after injury. Oval or polygonal fibroblastsare mainly distributed in the upper part of the dermis. OF; oval or polygonal typefibroblast, SF; slim type fibroblast. Scale bar 10 µm. G.Electron micrograph of a fibroblast in a 15 day-old baby, 6 days after injury.Abundant endoplasmic reticula are distributed in the cytoplasm. Collagen fibersare gathered into bundles in the intercellular space. Scale bar 1µm. H. Electron micrograph of a granulation-like tissue in a 15day-old baby, 6 days after injury. The tissue was observed in a dermis and showeda compact packing group of many fibroblasts and some macrophage in it. MF;macrophage Scale bar 2 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4548005&req=5

fig_005: A. Electron micrograph of a scab and its vicinity in a 15-day old baby 1 dayafter injury. The cell component is not confirmed, and vesicles are distributedover the scab. Cells contacting the scab are of unknown origin. SCAB; scab. Scalebar 2µm. B. Electron micrograph of the wall of a wound in a15-day old baby, 1 day after injury. Cells which seem to be extending from thewound area are distributed at the wound wall. The place in the upper region wheresquamous cells are not distributed is the edge of the wound (arrow). Scale bar 2µm. C. Electron micrograph of a scab and an epidermis in a 15day-old baby, 3 days after injury. Keratinocyte cells or layers are not observed,but the epidermis covers the wound. SCAB; scab Scale bar 2 µm. D.Electron micrograph of a dermis in a 15 day-old baby, 3 days after injury. Bundlesof fibers and fibroblasts are densely distributed, and the intercellular space issmall. OF; oval or polygonal fibroblast, SF; slim fibroblast. Scale bar 2µm. E. Electron micrograph of an epidermis in a 15 day-oldbaby, 6 days after injury. Large squamous cells are arranged in layers. Basalcells are clearly distinguishable from squamous cells. BC; basal cell, SC;squamous cell. Scale bar 10 µm. F. Electron micrograph of adermis in a 15 day-old baby, 6 days after injury. Oval or polygonal fibroblastsare mainly distributed in the upper part of the dermis. OF; oval or polygonal typefibroblast, SF; slim type fibroblast. Scale bar 10 µm. G.Electron micrograph of a fibroblast in a 15 day-old baby, 6 days after injury.Abundant endoplasmic reticula are distributed in the cytoplasm. Collagen fibersare gathered into bundles in the intercellular space. Scale bar 1µm. H. Electron micrograph of a granulation-like tissue in a 15day-old baby, 6 days after injury. The tissue was observed in a dermis and showeda compact packing group of many fibroblasts and some macrophage in it. MF;macrophage Scale bar 2 µm.
Mentions: The wounded area was filled with scab, and vesicles and a homogeneous framework wasdistributed beneath the scab. The origin of the vesicles and the framework was unknown,but both were presumably secreted by cells neighboring the wall of the wound. Theframework contained abundant small vesicles and granules (Fig. 5AFig. 5.

Bottom Line: The quantity of extracellular matrix of the dermis increased thereafter, and several types of cells were found in the dermis.To examine the wound healing, we used material from a 1 day-old newborn baby, and from another 15 days after birth, and compared the wound healing style morphologically.Differences in the constitution of cells and fine structures of the skin were observed, it was obviously suggested that change in the wound healing style from fetal-type to adult-type occurred between 1 to 15 days after birth.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agriculture, Tottori University, Nishikawatsu 1060, Matsue, Shimane 690-8504, Japan.

ABSTRACT
The opossum delivers a newborn baby equivalent to tremature fetus state by postpregnancy. The peculiarity is advantageous for studies of fetus, because operations to take out fetus from the uterus of a mother are not necessary. When mammalian skin is wounded by full-thickness excision, fetal and adult wound healing processes differ. Fetal-type wound healing does not leave a scar. However, studies of how the fetal wound healing process differs in detail from the adult type are not advanced. We first observed the normal skin development of the gray short-tailed opossum (Monodelphis domestica) using an electron microscope. As for normal skin, an epidermis became multi-layered, and thickened from birth through to 7 days after birth. The quantity of extracellular matrix of the dermis increased thereafter, and several types of cells were found in the dermis. To examine the wound healing, we used material from a 1 day-old newborn baby, and from another 15 days after birth, and compared the wound healing style morphologically. Differences in the constitution of cells and fine structures of the skin were observed, it was obviously suggested that change in the wound healing style from fetal-type to adult-type occurred between 1 to 15 days after birth.

No MeSH data available.


Related in: MedlinePlus