Limits...
Ultrastructural analysis between fetal and adult wound healing process of marsupial opossum skin.

Matsuno K, Ihara S - Exp. Anim. (2015)

Bottom Line: We first observed the normal skin development of the gray short-tailed opossum (Monodelphis domestica) using an electron microscope.As for normal skin, an epidermis became multi-layered, and thickened from birth through to 7 days after birth.The quantity of extracellular matrix of the dermis increased thereafter, and several types of cells were found in the dermis.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agriculture, Tottori University, Nishikawatsu 1060, Matsue, Shimane 690-8504, Japan.

ABSTRACT
The opossum delivers a newborn baby equivalent to tremature fetus state by postpregnancy. The peculiarity is advantageous for studies of fetus, because operations to take out fetus from the uterus of a mother are not necessary. When mammalian skin is wounded by full-thickness excision, fetal and adult wound healing processes differ. Fetal-type wound healing does not leave a scar. However, studies of how the fetal wound healing process differs in detail from the adult type are not advanced. We first observed the normal skin development of the gray short-tailed opossum (Monodelphis domestica) using an electron microscope. As for normal skin, an epidermis became multi-layered, and thickened from birth through to 7 days after birth. The quantity of extracellular matrix of the dermis increased thereafter, and several types of cells were found in the dermis. To examine the wound healing, we used material from a 1 day-old newborn baby, and from another 15 days after birth, and compared the wound healing style morphologically. Differences in the constitution of cells and fine structures of the skin were observed, it was obviously suggested that change in the wound healing style from fetal-type to adult-type occurred between 1 to 15 days after birth.

No MeSH data available.


Related in: MedlinePlus

A. Electron micrograph of a keratinized layer of an opossum baby 0 day after birth:Many keratohyaline granules are included in the cells (arrow). Scale bar 1µm. B. Electron micrograph of an epidermis and a dermis of a baby0 day after birth: border between the epidermis and the dermis is clear, and twotypes of fibroblasts are observed within the dermis. OF; oval or polygonalfibroblast, SF; slim fibroblast. Scale bar 10 µm. C. Electronmicrograph of a fibroblast and the neighboring intercellular space from a baby 0 dayafter birth. The fibroblast contains compact packed endoplasmic reticula in itscytoplasm. Bundles of Collagen fibers are observed at the space. Scale bar 1µm. D. Enlarged view of the bundle which marked in Fig. 1C. The bundle is composed of somecollagen fibers. Scale bar 0.25 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4548005&req=5

fig_001: A. Electron micrograph of a keratinized layer of an opossum baby 0 day after birth:Many keratohyaline granules are included in the cells (arrow). Scale bar 1µm. B. Electron micrograph of an epidermis and a dermis of a baby0 day after birth: border between the epidermis and the dermis is clear, and twotypes of fibroblasts are observed within the dermis. OF; oval or polygonalfibroblast, SF; slim fibroblast. Scale bar 10 µm. C. Electronmicrograph of a fibroblast and the neighboring intercellular space from a baby 0 dayafter birth. The fibroblast contains compact packed endoplasmic reticula in itscytoplasm. Bundles of Collagen fibers are observed at the space. Scale bar 1µm. D. Enlarged view of the bundle which marked in Fig. 1C. The bundle is composed of somecollagen fibers. Scale bar 0.25 µm.

Mentions: The skin of the newborn opossum at 0 day after birth was clearly divided into anepidermis and a dermis. Three types of cells were apparent. One or two layers of flatkeratinocytes containing keratohyaline granules were arranged at the outermost part of theepidermis, forming a keratinized layer (Figs.1A and 1BFig. 1.


Ultrastructural analysis between fetal and adult wound healing process of marsupial opossum skin.

Matsuno K, Ihara S - Exp. Anim. (2015)

A. Electron micrograph of a keratinized layer of an opossum baby 0 day after birth:Many keratohyaline granules are included in the cells (arrow). Scale bar 1µm. B. Electron micrograph of an epidermis and a dermis of a baby0 day after birth: border between the epidermis and the dermis is clear, and twotypes of fibroblasts are observed within the dermis. OF; oval or polygonalfibroblast, SF; slim fibroblast. Scale bar 10 µm. C. Electronmicrograph of a fibroblast and the neighboring intercellular space from a baby 0 dayafter birth. The fibroblast contains compact packed endoplasmic reticula in itscytoplasm. Bundles of Collagen fibers are observed at the space. Scale bar 1µm. D. Enlarged view of the bundle which marked in Fig. 1C. The bundle is composed of somecollagen fibers. Scale bar 0.25 µm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4548005&req=5

fig_001: A. Electron micrograph of a keratinized layer of an opossum baby 0 day after birth:Many keratohyaline granules are included in the cells (arrow). Scale bar 1µm. B. Electron micrograph of an epidermis and a dermis of a baby0 day after birth: border between the epidermis and the dermis is clear, and twotypes of fibroblasts are observed within the dermis. OF; oval or polygonalfibroblast, SF; slim fibroblast. Scale bar 10 µm. C. Electronmicrograph of a fibroblast and the neighboring intercellular space from a baby 0 dayafter birth. The fibroblast contains compact packed endoplasmic reticula in itscytoplasm. Bundles of Collagen fibers are observed at the space. Scale bar 1µm. D. Enlarged view of the bundle which marked in Fig. 1C. The bundle is composed of somecollagen fibers. Scale bar 0.25 µm.
Mentions: The skin of the newborn opossum at 0 day after birth was clearly divided into anepidermis and a dermis. Three types of cells were apparent. One or two layers of flatkeratinocytes containing keratohyaline granules were arranged at the outermost part of theepidermis, forming a keratinized layer (Figs.1A and 1BFig. 1.

Bottom Line: We first observed the normal skin development of the gray short-tailed opossum (Monodelphis domestica) using an electron microscope.As for normal skin, an epidermis became multi-layered, and thickened from birth through to 7 days after birth.The quantity of extracellular matrix of the dermis increased thereafter, and several types of cells were found in the dermis.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agriculture, Tottori University, Nishikawatsu 1060, Matsue, Shimane 690-8504, Japan.

ABSTRACT
The opossum delivers a newborn baby equivalent to tremature fetus state by postpregnancy. The peculiarity is advantageous for studies of fetus, because operations to take out fetus from the uterus of a mother are not necessary. When mammalian skin is wounded by full-thickness excision, fetal and adult wound healing processes differ. Fetal-type wound healing does not leave a scar. However, studies of how the fetal wound healing process differs in detail from the adult type are not advanced. We first observed the normal skin development of the gray short-tailed opossum (Monodelphis domestica) using an electron microscope. As for normal skin, an epidermis became multi-layered, and thickened from birth through to 7 days after birth. The quantity of extracellular matrix of the dermis increased thereafter, and several types of cells were found in the dermis. To examine the wound healing, we used material from a 1 day-old newborn baby, and from another 15 days after birth, and compared the wound healing style morphologically. Differences in the constitution of cells and fine structures of the skin were observed, it was obviously suggested that change in the wound healing style from fetal-type to adult-type occurred between 1 to 15 days after birth.

No MeSH data available.


Related in: MedlinePlus