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Dietary Milk Sphingomyelin Prevents Disruption of Skin Barrier Function in Hairless Mice after UV-B Irradiation.

Oba C, Morifuji M, Ichikawa S, Ito K, Kawahata K, Yamaji T, Asami Y, Itou H, Sugawara T - PLoS ONE (2015)

Bottom Line: Administration of SM significantly suppressed an increase in transepidermal water loss and a decrease in SC water content induced by UV-B irradiation.SM supplementation significantly maintained covalently-bound ω-hydroxy ceramide levels and down-regulated mRNA levels of acute inflammation-associated genes, including thymic stromal lymphopoietin, interleukin-1 beta, and interleukin-6.Furthermore, significantly higher levels of loricrin and transglutaminase-3 mRNA were observed in the SM group.

View Article: PubMed Central - PubMed

Affiliation: Food Science Research Labs, Meiji Co., Ltd., 540 Naruda, Odawara-shi, Kanagawa, 250-0862, Japan.

ABSTRACT
Exposure to ultraviolet-B (UV-B) irradiation causes skin barrier defects. Based on earlier findings that milk phospholipids containing high amounts of sphingomyelin (SM) improved the water content of the stratum corneum (SC) in normal mice, here we investigated the effects of dietary milk SM on skin barrier defects induced by a single dose of UV-B irradiation in hairless mice. Nine week old hairless mice were orally administrated SM (146 mg/kg BW/day) for a total of ten days. After seven days of SM administration, the dorsal skin was exposed to a single dose of UV-B (20 mJ/cm2). Administration of SM significantly suppressed an increase in transepidermal water loss and a decrease in SC water content induced by UV-B irradiation. SM supplementation significantly maintained covalently-bound ω-hydroxy ceramide levels and down-regulated mRNA levels of acute inflammation-associated genes, including thymic stromal lymphopoietin, interleukin-1 beta, and interleukin-6. Furthermore, significantly higher levels of loricrin and transglutaminase-3 mRNA were observed in the SM group. Our study shows for the first time that dietary SM modulates epidermal structures, and can help prevent disruption of skin barrier function after UV-B irradiation.

No MeSH data available.


Related in: MedlinePlus

Effect of sphingomyelin on expression levels of TSLP (A), IL-1 beta (B) and IL-6 (C).(A) Thymic stromal lymphopoietin (TSLP); (B) Interleukin-1 (IL-1) beta; (C) Interleukin-6 (IL-6). The mRNA levels are expressed as fold-change ± SD (SD of ΔCt) (n = 8/group). *: P < 0.05 (vs. the control group). #: P < 0.05 (vs. day 0).
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pone.0136377.g003: Effect of sphingomyelin on expression levels of TSLP (A), IL-1 beta (B) and IL-6 (C).(A) Thymic stromal lymphopoietin (TSLP); (B) Interleukin-1 (IL-1) beta; (C) Interleukin-6 (IL-6). The mRNA levels are expressed as fold-change ± SD (SD of ΔCt) (n = 8/group). *: P < 0.05 (vs. the control group). #: P < 0.05 (vs. day 0).

Mentions: A significant up-regulation of TSLP, IL-1 beta, and IL-6 mRNA was observed in the control group on day 1 and 2, while TSLP and IL-6 mRNA levels were significantly increased in the SM group at every time point assessed (Fig 3). There was no change in the levels of IL-1 beta mRNA in the SM group after UV-B irradiation. The mRNA level of TSLP was significantly higher in the control group than the SM group on days 0 and 1, while the mRNA level of IL-1 beta on days 1 and 2 was markedly decreased for the control group and relatively constant for the SM group (Fig 3A and 3B). Meanwhile, IL-6 levels increased in the control group on day 1 after UV-B irradiation before tapering on days 2 and 3, while the SM group showed a similar expression pattern but with lower levels of mRNA (Fig 3C).


Dietary Milk Sphingomyelin Prevents Disruption of Skin Barrier Function in Hairless Mice after UV-B Irradiation.

Oba C, Morifuji M, Ichikawa S, Ito K, Kawahata K, Yamaji T, Asami Y, Itou H, Sugawara T - PLoS ONE (2015)

Effect of sphingomyelin on expression levels of TSLP (A), IL-1 beta (B) and IL-6 (C).(A) Thymic stromal lymphopoietin (TSLP); (B) Interleukin-1 (IL-1) beta; (C) Interleukin-6 (IL-6). The mRNA levels are expressed as fold-change ± SD (SD of ΔCt) (n = 8/group). *: P < 0.05 (vs. the control group). #: P < 0.05 (vs. day 0).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4547804&req=5

pone.0136377.g003: Effect of sphingomyelin on expression levels of TSLP (A), IL-1 beta (B) and IL-6 (C).(A) Thymic stromal lymphopoietin (TSLP); (B) Interleukin-1 (IL-1) beta; (C) Interleukin-6 (IL-6). The mRNA levels are expressed as fold-change ± SD (SD of ΔCt) (n = 8/group). *: P < 0.05 (vs. the control group). #: P < 0.05 (vs. day 0).
Mentions: A significant up-regulation of TSLP, IL-1 beta, and IL-6 mRNA was observed in the control group on day 1 and 2, while TSLP and IL-6 mRNA levels were significantly increased in the SM group at every time point assessed (Fig 3). There was no change in the levels of IL-1 beta mRNA in the SM group after UV-B irradiation. The mRNA level of TSLP was significantly higher in the control group than the SM group on days 0 and 1, while the mRNA level of IL-1 beta on days 1 and 2 was markedly decreased for the control group and relatively constant for the SM group (Fig 3A and 3B). Meanwhile, IL-6 levels increased in the control group on day 1 after UV-B irradiation before tapering on days 2 and 3, while the SM group showed a similar expression pattern but with lower levels of mRNA (Fig 3C).

Bottom Line: Administration of SM significantly suppressed an increase in transepidermal water loss and a decrease in SC water content induced by UV-B irradiation.SM supplementation significantly maintained covalently-bound ω-hydroxy ceramide levels and down-regulated mRNA levels of acute inflammation-associated genes, including thymic stromal lymphopoietin, interleukin-1 beta, and interleukin-6.Furthermore, significantly higher levels of loricrin and transglutaminase-3 mRNA were observed in the SM group.

View Article: PubMed Central - PubMed

Affiliation: Food Science Research Labs, Meiji Co., Ltd., 540 Naruda, Odawara-shi, Kanagawa, 250-0862, Japan.

ABSTRACT
Exposure to ultraviolet-B (UV-B) irradiation causes skin barrier defects. Based on earlier findings that milk phospholipids containing high amounts of sphingomyelin (SM) improved the water content of the stratum corneum (SC) in normal mice, here we investigated the effects of dietary milk SM on skin barrier defects induced by a single dose of UV-B irradiation in hairless mice. Nine week old hairless mice were orally administrated SM (146 mg/kg BW/day) for a total of ten days. After seven days of SM administration, the dorsal skin was exposed to a single dose of UV-B (20 mJ/cm2). Administration of SM significantly suppressed an increase in transepidermal water loss and a decrease in SC water content induced by UV-B irradiation. SM supplementation significantly maintained covalently-bound ω-hydroxy ceramide levels and down-regulated mRNA levels of acute inflammation-associated genes, including thymic stromal lymphopoietin, interleukin-1 beta, and interleukin-6. Furthermore, significantly higher levels of loricrin and transglutaminase-3 mRNA were observed in the SM group. Our study shows for the first time that dietary SM modulates epidermal structures, and can help prevent disruption of skin barrier function after UV-B irradiation.

No MeSH data available.


Related in: MedlinePlus