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Cortex Fraxini (Qingpi) Protects Rat Pheochromocytoma Cells against 6-Hydroxydopamine-Induced Apoptosis.

Li JJ, Zhou SY, Zhang H, Lam KH, Lee SM, Yu PH, Chan SW - Parkinsons Dis (2015)

Bottom Line: It was found that QP water extract possesses strong antioxidant property with SC50 = 0.15 mg/mL.Total phenolic content of QP water extract was found to be 200.78 ± 2.65 mg GAE/g.The protective effect of QP water extract was found to be via inhibiting MEK/ERK pathway and reversing PI3-K/Akt/GSK3β pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong ; School of Biological Sciences, The University of Hong Kong, Hong Kong.

ABSTRACT
Parkinson's disease (PD) is a chronic neurodegenerative disorder having close relationship with oxidative stress induced by reactive oxygen species (ROS). Cortex Fraxini (QP) is a kind of traditional Chinese medicinal herb with antioxidant properties. It may be a potential candidate for preventing the development of chronic neurodegenerative diseases. Thus, the key objective of the current study was to investigate the neuroprotective effect of QP water extract on 6-hydroxydopamine (6-OHDA) induced apoptosis in rat pheochromocytoma (PC12) cells. It was found that QP water extract possesses strong antioxidant property with SC50 = 0.15 mg/mL. Total phenolic content of QP water extract was found to be 200.78 ± 2.65 mg GAE/g. QP water extract's free radical scavenging capacity was demonstrated by reversing the increased level of intracellular ROS induced by 6-OHDA, using 2',7'-dichlorodihydrofluorescein diacetate. Moreover, QP water extract (0.5 mg/mL) could remarkably increase the viability of PC12 cells treated with 6-OHDA. The protective effect of QP water extract was found to be via inhibiting MEK/ERK pathway and reversing PI3-K/Akt/GSK3β pathway. The current results suggest that QP might be a potential candidate for preventing the development of neurodegenerative diseases, such as PD.

No MeSH data available.


Related in: MedlinePlus

QP water extract attenuated 6-OHDA-induced apoptosis in PC12 cells. (a) Effects of QP water extract (0.5 mg/mL) on the changes of mitochondrial membrane potential induced by 6-OHDA (500 μM). (b) Statistical analysis of the red/green fluorescence intensity ratio, namely mitochondrial membrane potential, expressed as the percentage of control. Data were expressed as means ± SEM of three separate experiments;  ###P < 0.001 versus control and  ∗∗∗P < 0.001 versus 6-OHDA alone group.
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fig6: QP water extract attenuated 6-OHDA-induced apoptosis in PC12 cells. (a) Effects of QP water extract (0.5 mg/mL) on the changes of mitochondrial membrane potential induced by 6-OHDA (500 μM). (b) Statistical analysis of the red/green fluorescence intensity ratio, namely mitochondrial membrane potential, expressed as the percentage of control. Data were expressed as means ± SEM of three separate experiments;  ###P < 0.001 versus control and  ∗∗∗P < 0.001 versus 6-OHDA alone group.

Mentions: Nucleus staining assay with Hoechst 33342 was taken to evaluate the morphological changes, which indicated apoptosis in PC12 cells. After observing cells under the fluorescence microscope (×400 magnification), compared with the morphological character in control group, the group treated with 6-OHDA (500 μM, 2 h) exhibited the symptom of apoptosis, including shrinkage of nuclei and condensation of chromatin appearance of a few apoptotic bodies and less number of cells (Figure 5). In contrast, cells pretreated with QP water extract (0.5 mg/mL, 2 h) showed less signs of apoptosis (Figure 5). Additionally, flow cytometry was used to further assess the apoptosis of PC12 cells through the measurement of mitochondrial membrane potential. As shown in Figure 6(a), pretreatment with QP water extract (0.5 mg/mL, 2 h) could reverse the PC12 mitochondrial membrane dysfunction induced by 6-OHDA (500 μM, 4 h) (Figure 6(b)).


Cortex Fraxini (Qingpi) Protects Rat Pheochromocytoma Cells against 6-Hydroxydopamine-Induced Apoptosis.

Li JJ, Zhou SY, Zhang H, Lam KH, Lee SM, Yu PH, Chan SW - Parkinsons Dis (2015)

QP water extract attenuated 6-OHDA-induced apoptosis in PC12 cells. (a) Effects of QP water extract (0.5 mg/mL) on the changes of mitochondrial membrane potential induced by 6-OHDA (500 μM). (b) Statistical analysis of the red/green fluorescence intensity ratio, namely mitochondrial membrane potential, expressed as the percentage of control. Data were expressed as means ± SEM of three separate experiments;  ###P < 0.001 versus control and  ∗∗∗P < 0.001 versus 6-OHDA alone group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4546978&req=5

fig6: QP water extract attenuated 6-OHDA-induced apoptosis in PC12 cells. (a) Effects of QP water extract (0.5 mg/mL) on the changes of mitochondrial membrane potential induced by 6-OHDA (500 μM). (b) Statistical analysis of the red/green fluorescence intensity ratio, namely mitochondrial membrane potential, expressed as the percentage of control. Data were expressed as means ± SEM of three separate experiments;  ###P < 0.001 versus control and  ∗∗∗P < 0.001 versus 6-OHDA alone group.
Mentions: Nucleus staining assay with Hoechst 33342 was taken to evaluate the morphological changes, which indicated apoptosis in PC12 cells. After observing cells under the fluorescence microscope (×400 magnification), compared with the morphological character in control group, the group treated with 6-OHDA (500 μM, 2 h) exhibited the symptom of apoptosis, including shrinkage of nuclei and condensation of chromatin appearance of a few apoptotic bodies and less number of cells (Figure 5). In contrast, cells pretreated with QP water extract (0.5 mg/mL, 2 h) showed less signs of apoptosis (Figure 5). Additionally, flow cytometry was used to further assess the apoptosis of PC12 cells through the measurement of mitochondrial membrane potential. As shown in Figure 6(a), pretreatment with QP water extract (0.5 mg/mL, 2 h) could reverse the PC12 mitochondrial membrane dysfunction induced by 6-OHDA (500 μM, 4 h) (Figure 6(b)).

Bottom Line: It was found that QP water extract possesses strong antioxidant property with SC50 = 0.15 mg/mL.Total phenolic content of QP water extract was found to be 200.78 ± 2.65 mg GAE/g.The protective effect of QP water extract was found to be via inhibiting MEK/ERK pathway and reversing PI3-K/Akt/GSK3β pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong ; School of Biological Sciences, The University of Hong Kong, Hong Kong.

ABSTRACT
Parkinson's disease (PD) is a chronic neurodegenerative disorder having close relationship with oxidative stress induced by reactive oxygen species (ROS). Cortex Fraxini (QP) is a kind of traditional Chinese medicinal herb with antioxidant properties. It may be a potential candidate for preventing the development of chronic neurodegenerative diseases. Thus, the key objective of the current study was to investigate the neuroprotective effect of QP water extract on 6-hydroxydopamine (6-OHDA) induced apoptosis in rat pheochromocytoma (PC12) cells. It was found that QP water extract possesses strong antioxidant property with SC50 = 0.15 mg/mL. Total phenolic content of QP water extract was found to be 200.78 ± 2.65 mg GAE/g. QP water extract's free radical scavenging capacity was demonstrated by reversing the increased level of intracellular ROS induced by 6-OHDA, using 2',7'-dichlorodihydrofluorescein diacetate. Moreover, QP water extract (0.5 mg/mL) could remarkably increase the viability of PC12 cells treated with 6-OHDA. The protective effect of QP water extract was found to be via inhibiting MEK/ERK pathway and reversing PI3-K/Akt/GSK3β pathway. The current results suggest that QP might be a potential candidate for preventing the development of neurodegenerative diseases, such as PD.

No MeSH data available.


Related in: MedlinePlus