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miR-181c associates with tumor relapse of high grade osteosarcoma.

Mori F, Sacconi A, Canu V, Ganci F, Novello M, Anelli V, Covello R, Ferraresi V, Muti P, Biagini R, Blandino G, Strano S - Oncotarget (2015)

Bottom Line: We found that most of modulated miRs were associated with pathways of bone resorption and osteogenesis. miRNA expression also revealed that GCT and OS were distinct tumors.Interestingly, we found that miR-181c and other three miRNAs identified in the first step of the study were also consistently de-regulated in all OS patients.Ectopic expression of miR-181c reduced cell viability and enhanced chemotherapeutic-induced cell death of U2OS and SAOS2 cells.

View Article: PubMed Central - PubMed

Affiliation: Molecular Chemoprevention Unit, Regina Elena National Cancer Institute, Rome, Italy.

ABSTRACT
High-grade osteosarcoma (OS) is characterized by low incidence, high aggressiveness and moderate 5-years survival rate after aggressive poly-chemotherapy and surgery. Here we used miRNA profiling as a tool to possibly predict and monitor OS's development and therapeutic outcome. First, we evaluated the altered expression of selected miRNAs from a case of Giant Cell Tumor (GCT) apparently evolved into an OS. We found that most of modulated miRs were associated with pathways of bone resorption and osteogenesis. miRNA expression also revealed that GCT and OS were distinct tumors. Second, we validated the observed miRNA profile in two independent casuistries of ten GCT (not evolved into malignant tumors) and sixteen OS patients. Interestingly, we found that miR-181c and other three miRNAs identified in the first step of the study were also consistently de-regulated in all OS patients. Ectopic expression of miR-181c reduced cell viability and enhanced chemotherapeutic-induced cell death of U2OS and SAOS2 cells. These findings indicate that: i) miRNAs aberrantly modulated in GCT could be predictive of its development into OS and ii) miRNAs expression could be useful to monitor the OS therapeutic outcome.

No MeSH data available.


Related in: MedlinePlus

miRNA expression levels in IRE Osteosarcoma casuistrymiR-181a, miR193a, miR-181c, miR-378 and miR-198 expression levels in ten GCTs versus sixteen OSs from IRE casuistry. Four out of five miRNAs are down-regulated in GCTs respect to OSs. Asterisks indicate the expression levels relative to case report GCT and case report OS: they are distributed within the values of the GCTs' and OS' samples respectively.
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Figure 4: miRNA expression levels in IRE Osteosarcoma casuistrymiR-181a, miR193a, miR-181c, miR-378 and miR-198 expression levels in ten GCTs versus sixteen OSs from IRE casuistry. Four out of five miRNAs are down-regulated in GCTs respect to OSs. Asterisks indicate the expression levels relative to case report GCT and case report OS: they are distributed within the values of the GCTs' and OS' samples respectively.

Mentions: qRT-PCR analysis revealed that four out of five miRNAs identified in the case report analysis, were significantly down-regulated in the analyzed OS casuistry respect to GCT one (Fig. 4) and the patient A GCT and OS (asterisks) are distributed within the values of the GCT's and OS's samples respectively.


miR-181c associates with tumor relapse of high grade osteosarcoma.

Mori F, Sacconi A, Canu V, Ganci F, Novello M, Anelli V, Covello R, Ferraresi V, Muti P, Biagini R, Blandino G, Strano S - Oncotarget (2015)

miRNA expression levels in IRE Osteosarcoma casuistrymiR-181a, miR193a, miR-181c, miR-378 and miR-198 expression levels in ten GCTs versus sixteen OSs from IRE casuistry. Four out of five miRNAs are down-regulated in GCTs respect to OSs. Asterisks indicate the expression levels relative to case report GCT and case report OS: they are distributed within the values of the GCTs' and OS' samples respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4546443&req=5

Figure 4: miRNA expression levels in IRE Osteosarcoma casuistrymiR-181a, miR193a, miR-181c, miR-378 and miR-198 expression levels in ten GCTs versus sixteen OSs from IRE casuistry. Four out of five miRNAs are down-regulated in GCTs respect to OSs. Asterisks indicate the expression levels relative to case report GCT and case report OS: they are distributed within the values of the GCTs' and OS' samples respectively.
Mentions: qRT-PCR analysis revealed that four out of five miRNAs identified in the case report analysis, were significantly down-regulated in the analyzed OS casuistry respect to GCT one (Fig. 4) and the patient A GCT and OS (asterisks) are distributed within the values of the GCT's and OS's samples respectively.

Bottom Line: We found that most of modulated miRs were associated with pathways of bone resorption and osteogenesis. miRNA expression also revealed that GCT and OS were distinct tumors.Interestingly, we found that miR-181c and other three miRNAs identified in the first step of the study were also consistently de-regulated in all OS patients.Ectopic expression of miR-181c reduced cell viability and enhanced chemotherapeutic-induced cell death of U2OS and SAOS2 cells.

View Article: PubMed Central - PubMed

Affiliation: Molecular Chemoprevention Unit, Regina Elena National Cancer Institute, Rome, Italy.

ABSTRACT
High-grade osteosarcoma (OS) is characterized by low incidence, high aggressiveness and moderate 5-years survival rate after aggressive poly-chemotherapy and surgery. Here we used miRNA profiling as a tool to possibly predict and monitor OS's development and therapeutic outcome. First, we evaluated the altered expression of selected miRNAs from a case of Giant Cell Tumor (GCT) apparently evolved into an OS. We found that most of modulated miRs were associated with pathways of bone resorption and osteogenesis. miRNA expression also revealed that GCT and OS were distinct tumors. Second, we validated the observed miRNA profile in two independent casuistries of ten GCT (not evolved into malignant tumors) and sixteen OS patients. Interestingly, we found that miR-181c and other three miRNAs identified in the first step of the study were also consistently de-regulated in all OS patients. Ectopic expression of miR-181c reduced cell viability and enhanced chemotherapeutic-induced cell death of U2OS and SAOS2 cells. These findings indicate that: i) miRNAs aberrantly modulated in GCT could be predictive of its development into OS and ii) miRNAs expression could be useful to monitor the OS therapeutic outcome.

No MeSH data available.


Related in: MedlinePlus