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Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem.

Sengupta S, Pramanik A, Ghosh A, Bhattacharyya M - BMC Microbiol. (2015)

Bottom Line: Seventy-one carbon sources and twenty-three chemical sources utilization assay revealed their metabolic relatedness.Among these nine isolates three specific strains were found to have notably higher degree of antimicrobial potential effective in a broader range including phyto-pathogenic fungus.Antimicrobial compound has also been identified and purified which is active against a broad range of pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, India.

ABSTRACT

Background: New broad spectrum antimicrobial agents are urgently needed to combat frequently emerging multi drug resistant pathogens. Actinomycetes, the most talented group of microorganisms isolated from unexplored regions of the world may be the ultimate solution to this problem. Thus the aim of this study was to isolate several bioactive actinomycetes strains capable of producing antimicrobial secondary metabolite from Sundarbans, the only mangrove tiger land of the world.

Results: Fifty four actinomycetes were isolated and analyzed for antimicrobial activity against fifteen test organisms including three phytopathogens. Nine morphologically distinct and biologically active isolates were subjected to polyphasic identification study.16 s rDNA sequencing indicated eight isolates to reveal maximum similarity to the genus streptomyces, whereas one isolate presented only 93.57% similarity with Streptomyces albogriseolus NRRL B-1305(T). Seventy-one carbon sources and twenty-three chemical sources utilization assay revealed their metabolic relatedness. Among these nine isolates three specific strains were found to have notably higher degree of antimicrobial potential effective in a broader range including phyto-pathogenic fungus. Finally the strain SMS_SU21, which showed antimicrobial activity with MIC value of 0.05 mg ml(-1) and antioxidant activity with IC50 value of 0.242 ± 0.33 mg ml(-1) was detected to be the most potential one. True prospective of this strain was evaluated utilizing GC-MS and the bioactive compound responsible for antimicrobial activity was purified.

Conclusion: Rare bioactive actinomycetes were isolated from unexplored heritage site. Antimicrobial compound has also been identified and purified which is active against a broad range of pathogens.

No MeSH data available.


Related in: MedlinePlus

Panel 1- Morphology of sporophores of nine Actinomycetes studied by scanning electron microscopy [(a) SMS_B, (b) SMS_5, (c) SMS_7, (d) SMS_9, (e) SMS_10, (f) SMS_13, (g) SMS_SU13, (h) SMS_SU21, (i) SMS_SU23]. Scale bar shown on each photomicrograph. Panel 2- Arrangement of mycelium studied by phase contrast microscopy. [Phase contrast micrographs of nine actinomycets (a) SMS_B (b) SMS_5 (c) SMS_7 (d) SMS_9 (e) SMS_10 (f) SMS_13 (g) SMS_SU13 (h) SMS_SU21 (i) SMS_SU23 . Magnification 40 × .]
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Fig1: Panel 1- Morphology of sporophores of nine Actinomycetes studied by scanning electron microscopy [(a) SMS_B, (b) SMS_5, (c) SMS_7, (d) SMS_9, (e) SMS_10, (f) SMS_13, (g) SMS_SU13, (h) SMS_SU21, (i) SMS_SU23]. Scale bar shown on each photomicrograph. Panel 2- Arrangement of mycelium studied by phase contrast microscopy. [Phase contrast micrographs of nine actinomycets (a) SMS_B (b) SMS_5 (c) SMS_7 (d) SMS_9 (e) SMS_10 (f) SMS_13 (g) SMS_SU13 (h) SMS_SU21 (i) SMS_SU23 . Magnification 40 × .]

Mentions: Seven selective media (Additional file 1: Figure S1 and Additional file 1: Table S3) were used to observe the distinct colony morphology of the isolates. The colours of the aerial and substrate mycelium were described according to the colours of the RALcode. Aerial hyphae arrangements, Spore chain ornamentation and spore surface of the isolates were subsequently observed by phase contrast and scanning electron microscopy (Fig. 1), indicating variation among the sporophore sizes and ornamentation and even in the spore surface, especially SMS_10 possessing a unique bouquet like spore arrangement.Fig. 1


Antimicrobial activities of actinomycetes isolated from unexplored regions of Sundarbans mangrove ecosystem.

Sengupta S, Pramanik A, Ghosh A, Bhattacharyya M - BMC Microbiol. (2015)

Panel 1- Morphology of sporophores of nine Actinomycetes studied by scanning electron microscopy [(a) SMS_B, (b) SMS_5, (c) SMS_7, (d) SMS_9, (e) SMS_10, (f) SMS_13, (g) SMS_SU13, (h) SMS_SU21, (i) SMS_SU23]. Scale bar shown on each photomicrograph. Panel 2- Arrangement of mycelium studied by phase contrast microscopy. [Phase contrast micrographs of nine actinomycets (a) SMS_B (b) SMS_5 (c) SMS_7 (d) SMS_9 (e) SMS_10 (f) SMS_13 (g) SMS_SU13 (h) SMS_SU21 (i) SMS_SU23 . Magnification 40 × .]
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4546244&req=5

Fig1: Panel 1- Morphology of sporophores of nine Actinomycetes studied by scanning electron microscopy [(a) SMS_B, (b) SMS_5, (c) SMS_7, (d) SMS_9, (e) SMS_10, (f) SMS_13, (g) SMS_SU13, (h) SMS_SU21, (i) SMS_SU23]. Scale bar shown on each photomicrograph. Panel 2- Arrangement of mycelium studied by phase contrast microscopy. [Phase contrast micrographs of nine actinomycets (a) SMS_B (b) SMS_5 (c) SMS_7 (d) SMS_9 (e) SMS_10 (f) SMS_13 (g) SMS_SU13 (h) SMS_SU21 (i) SMS_SU23 . Magnification 40 × .]
Mentions: Seven selective media (Additional file 1: Figure S1 and Additional file 1: Table S3) were used to observe the distinct colony morphology of the isolates. The colours of the aerial and substrate mycelium were described according to the colours of the RALcode. Aerial hyphae arrangements, Spore chain ornamentation and spore surface of the isolates were subsequently observed by phase contrast and scanning electron microscopy (Fig. 1), indicating variation among the sporophore sizes and ornamentation and even in the spore surface, especially SMS_10 possessing a unique bouquet like spore arrangement.Fig. 1

Bottom Line: Seventy-one carbon sources and twenty-three chemical sources utilization assay revealed their metabolic relatedness.Among these nine isolates three specific strains were found to have notably higher degree of antimicrobial potential effective in a broader range including phyto-pathogenic fungus.Antimicrobial compound has also been identified and purified which is active against a broad range of pathogens.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, India.

ABSTRACT

Background: New broad spectrum antimicrobial agents are urgently needed to combat frequently emerging multi drug resistant pathogens. Actinomycetes, the most talented group of microorganisms isolated from unexplored regions of the world may be the ultimate solution to this problem. Thus the aim of this study was to isolate several bioactive actinomycetes strains capable of producing antimicrobial secondary metabolite from Sundarbans, the only mangrove tiger land of the world.

Results: Fifty four actinomycetes were isolated and analyzed for antimicrobial activity against fifteen test organisms including three phytopathogens. Nine morphologically distinct and biologically active isolates were subjected to polyphasic identification study.16 s rDNA sequencing indicated eight isolates to reveal maximum similarity to the genus streptomyces, whereas one isolate presented only 93.57% similarity with Streptomyces albogriseolus NRRL B-1305(T). Seventy-one carbon sources and twenty-three chemical sources utilization assay revealed their metabolic relatedness. Among these nine isolates three specific strains were found to have notably higher degree of antimicrobial potential effective in a broader range including phyto-pathogenic fungus. Finally the strain SMS_SU21, which showed antimicrobial activity with MIC value of 0.05 mg ml(-1) and antioxidant activity with IC50 value of 0.242 ± 0.33 mg ml(-1) was detected to be the most potential one. True prospective of this strain was evaluated utilizing GC-MS and the bioactive compound responsible for antimicrobial activity was purified.

Conclusion: Rare bioactive actinomycetes were isolated from unexplored heritage site. Antimicrobial compound has also been identified and purified which is active against a broad range of pathogens.

No MeSH data available.


Related in: MedlinePlus