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Enhancement of Surfactin yield by improving the medium composition and fermentation process.

Willenbacher J, Yeremchuk W, Mohr T, Syldatk C, Hausmann R - AMB Express (2015)

Bottom Line: Surfactin is one of the most promising biosurfactants due to its extraordinary surface activity.Commonly, the well-established Cooper medium, a glucose-based mineral salt medium, is utilized for the microbial production of Surfactin.The following utilization of the enhanced medium composition during fed-batch fermentation with integrated foam fractionation yielded 30 % more Surfactin in comparison to batch fermentations with integrated foam fractionation employing the former version of the Cooper medium.

View Article: PubMed Central - PubMed

Affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology (KIT), Engler-Bunte-Ring 1, 76131, Karlsruhe, Germany, judit.willenbacher@kit.edu.

ABSTRACT
Surfactin is one of the most promising biosurfactants due to its extraordinary surface activity. Commonly, the well-established Cooper medium, a glucose-based mineral salt medium, is utilized for the microbial production of Surfactin. The current study investigated the enhancement of Surfactin yields by analyzing the effects of different glucose concentrations, next to the introduction of an alternative chelating agent and nitrogen source. The utilization of 8 g/L glucose, 0.008 mM Na3citrate and 50 mM (NH4)2SO4 increased Surfactin yields from 0.7 to 1.1 g/L during shake flask experiments applying Bacillus subtilis DSM10(T). Consequentially conducted shake flask experiments, employing five other Surfactin producer strains during cultivation in the former and enhanced version of the Cooper medium, suggest a general enhancement of Surfactin yields during application of the enhanced version of the Cooper medium. The enhancement of the medium composition is therefore most likely independent from the employed producer strain. The following utilization of the enhanced medium composition during fed-batch fermentation with integrated foam fractionation yielded 30 % more Surfactin in comparison to batch fermentations with integrated foam fractionation employing the former version of the Cooper medium.

No MeSH data available.


Related in: MedlinePlus

Time course of CDW and Surfactin concentrations of B. subtilis DSM 10T during shake flask cultivations in medium B and further optimized medium C. The achieved CDW [g/L] is shown in a, whereas resulting Surfactin concentrations [g/L] are illustrated in b. The results of cultivation in medium B are given as black dots. Data from cultivations in medium C are presented as white dots. The cultivations were conducted as duplicates and in time-displaced flasks to illustrate a continuous course of growth and Surfactin production
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Fig1: Time course of CDW and Surfactin concentrations of B. subtilis DSM 10T during shake flask cultivations in medium B and further optimized medium C. The achieved CDW [g/L] is shown in a, whereas resulting Surfactin concentrations [g/L] are illustrated in b. The results of cultivation in medium B are given as black dots. Data from cultivations in medium C are presented as white dots. The cultivations were conducted as duplicates and in time-displaced flasks to illustrate a continuous course of growth and Surfactin production

Mentions: Figure 1 compares the results of the former applied medium (slightly changed Cooper medium, Table 1: medium B) and the finally enhanced medium (Table 1: medium C). Time courses of the simultaneous cultivations of B. subtilis DSM 10T in the two different media reveal a maximal CDW of 2.4 g/L in medium B and 2.2 g/L in medium C. Bacterial growth terminated after 15 h of cultivation in medium C as the further optimized medium is glucose limited. Nevertheless, a significantly higher concentration of Surfactin was produced in the further optimized medium C, yielding a maximum of 1.1 g/L Surfactin. In contrast, cultivations in medium B reached 0.7 g/L as maximal Surfactin concentration. This comparison shows a significant enhancement in Surfactin yields after medium optimization during shake flask cultivations.Fig. 1


Enhancement of Surfactin yield by improving the medium composition and fermentation process.

Willenbacher J, Yeremchuk W, Mohr T, Syldatk C, Hausmann R - AMB Express (2015)

Time course of CDW and Surfactin concentrations of B. subtilis DSM 10T during shake flask cultivations in medium B and further optimized medium C. The achieved CDW [g/L] is shown in a, whereas resulting Surfactin concentrations [g/L] are illustrated in b. The results of cultivation in medium B are given as black dots. Data from cultivations in medium C are presented as white dots. The cultivations were conducted as duplicates and in time-displaced flasks to illustrate a continuous course of growth and Surfactin production
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4546119&req=5

Fig1: Time course of CDW and Surfactin concentrations of B. subtilis DSM 10T during shake flask cultivations in medium B and further optimized medium C. The achieved CDW [g/L] is shown in a, whereas resulting Surfactin concentrations [g/L] are illustrated in b. The results of cultivation in medium B are given as black dots. Data from cultivations in medium C are presented as white dots. The cultivations were conducted as duplicates and in time-displaced flasks to illustrate a continuous course of growth and Surfactin production
Mentions: Figure 1 compares the results of the former applied medium (slightly changed Cooper medium, Table 1: medium B) and the finally enhanced medium (Table 1: medium C). Time courses of the simultaneous cultivations of B. subtilis DSM 10T in the two different media reveal a maximal CDW of 2.4 g/L in medium B and 2.2 g/L in medium C. Bacterial growth terminated after 15 h of cultivation in medium C as the further optimized medium is glucose limited. Nevertheless, a significantly higher concentration of Surfactin was produced in the further optimized medium C, yielding a maximum of 1.1 g/L Surfactin. In contrast, cultivations in medium B reached 0.7 g/L as maximal Surfactin concentration. This comparison shows a significant enhancement in Surfactin yields after medium optimization during shake flask cultivations.Fig. 1

Bottom Line: Surfactin is one of the most promising biosurfactants due to its extraordinary surface activity.Commonly, the well-established Cooper medium, a glucose-based mineral salt medium, is utilized for the microbial production of Surfactin.The following utilization of the enhanced medium composition during fed-batch fermentation with integrated foam fractionation yielded 30 % more Surfactin in comparison to batch fermentations with integrated foam fractionation employing the former version of the Cooper medium.

View Article: PubMed Central - PubMed

Affiliation: Institute of Process Engineering in Life Sciences, Section II: Technical Biology, Karlsruhe Institute of Technology (KIT), Engler-Bunte-Ring 1, 76131, Karlsruhe, Germany, judit.willenbacher@kit.edu.

ABSTRACT
Surfactin is one of the most promising biosurfactants due to its extraordinary surface activity. Commonly, the well-established Cooper medium, a glucose-based mineral salt medium, is utilized for the microbial production of Surfactin. The current study investigated the enhancement of Surfactin yields by analyzing the effects of different glucose concentrations, next to the introduction of an alternative chelating agent and nitrogen source. The utilization of 8 g/L glucose, 0.008 mM Na3citrate and 50 mM (NH4)2SO4 increased Surfactin yields from 0.7 to 1.1 g/L during shake flask experiments applying Bacillus subtilis DSM10(T). Consequentially conducted shake flask experiments, employing five other Surfactin producer strains during cultivation in the former and enhanced version of the Cooper medium, suggest a general enhancement of Surfactin yields during application of the enhanced version of the Cooper medium. The enhancement of the medium composition is therefore most likely independent from the employed producer strain. The following utilization of the enhanced medium composition during fed-batch fermentation with integrated foam fractionation yielded 30 % more Surfactin in comparison to batch fermentations with integrated foam fractionation employing the former version of the Cooper medium.

No MeSH data available.


Related in: MedlinePlus