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GATA2 rs2335052 Polymorphism Predicts the Survival of Patients with Colorectal Cancer.

Liu X, Jiang B, Wang A, Di J, Wang Z, Chen L, Su X - PLoS ONE (2015)

Bottom Line: Moreover, univariate and multivariate Cox regression analyses revealed that GATA2 SNP rs2335052 was an independent risk factor for the DFS of CRC patients.Our results demonstrated that GATA2 SNP rs2335052 is an independent predictor for prognosis of CRC patients.This raised the possibility that SNP rs2335052 may serve as a potential indicator for predicting recurrence of CRC after curative colectomy.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Central Laboratory, Peking University Cancer Hospital & Institute, Beijing, China.

ABSTRACT

Background: GATA binding protein 2 (GATA2) is a transcription factor that has essential roles in hematologic malignancies and progression of various solid tumors. Our previous studies suggested that high GATA2 expression is associated with recurrence of colorectal cancer (CRC). However, the influence of GATA2 single nucleotide polymorphisms (SNPs) on the survival of CRC remains unknown.

Methods: We genotyped GATA2 SNP rs2335052 using Sanger sequencing after PCR amplification, and determined GATA2 expression by immunohistochemistry in a cohort of 180 CRC patients. Kaplan-Meier survival analysis and Cox proportional hazard regression were used to analyze the association between the GATA2 rs2335052 genotypes and the clinical outcome of CRC.

Results: We found that there was no significant correlation between the rs2335052 genotypes and the expression of GATA2. However, the Kaplan-Meier survival analysis suggested that the carriers of the A-allele of SNP rs2335052 were significantly associated with increased risk of recurrence and reduced disease-free survival (DFS), compared with those carrying the variant genotype of GG in rs2335052 (P = 0.021). Moreover, univariate and multivariate Cox regression analyses revealed that GATA2 SNP rs2335052 was an independent risk factor for the DFS of CRC patients.

Conclusion: Our results demonstrated that GATA2 SNP rs2335052 is an independent predictor for prognosis of CRC patients. This raised the possibility that SNP rs2335052 may serve as a potential indicator for predicting recurrence of CRC after curative colectomy.

No MeSH data available.


Related in: MedlinePlus

Sequencing results of GATA2 rs2335052 genotypes.
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pone.0136020.g001: Sequencing results of GATA2 rs2335052 genotypes.

Mentions: A total of 180 samples from patients with CRC were analyzed. Median follow up time in the cohort was 69.0 months. Carriers of the rs2335052 GG, GA, and AA genotypes were 64 (35.6%), 81 (45.0%), and 35 (19.4%), respectively (Fig 1). Details of the clinical characteristics and GATA2 rs2335052 genotypes are shown in Table 1. GATA2 SNP rs2335052 was significantly associated with disease-free survival (DFS) of CRC (P = 0.033). However, no significant associations were observed between rs2335052 genotypes and other clinicopathological characteristics (all P-values > 0.05).


GATA2 rs2335052 Polymorphism Predicts the Survival of Patients with Colorectal Cancer.

Liu X, Jiang B, Wang A, Di J, Wang Z, Chen L, Su X - PLoS ONE (2015)

Sequencing results of GATA2 rs2335052 genotypes.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4546112&req=5

pone.0136020.g001: Sequencing results of GATA2 rs2335052 genotypes.
Mentions: A total of 180 samples from patients with CRC were analyzed. Median follow up time in the cohort was 69.0 months. Carriers of the rs2335052 GG, GA, and AA genotypes were 64 (35.6%), 81 (45.0%), and 35 (19.4%), respectively (Fig 1). Details of the clinical characteristics and GATA2 rs2335052 genotypes are shown in Table 1. GATA2 SNP rs2335052 was significantly associated with disease-free survival (DFS) of CRC (P = 0.033). However, no significant associations were observed between rs2335052 genotypes and other clinicopathological characteristics (all P-values > 0.05).

Bottom Line: Moreover, univariate and multivariate Cox regression analyses revealed that GATA2 SNP rs2335052 was an independent risk factor for the DFS of CRC patients.Our results demonstrated that GATA2 SNP rs2335052 is an independent predictor for prognosis of CRC patients.This raised the possibility that SNP rs2335052 may serve as a potential indicator for predicting recurrence of CRC after curative colectomy.

View Article: PubMed Central - PubMed

Affiliation: Key laboratory of Carcinogenesis and Translational Research (Ministry of Education), Central Laboratory, Peking University Cancer Hospital & Institute, Beijing, China.

ABSTRACT

Background: GATA binding protein 2 (GATA2) is a transcription factor that has essential roles in hematologic malignancies and progression of various solid tumors. Our previous studies suggested that high GATA2 expression is associated with recurrence of colorectal cancer (CRC). However, the influence of GATA2 single nucleotide polymorphisms (SNPs) on the survival of CRC remains unknown.

Methods: We genotyped GATA2 SNP rs2335052 using Sanger sequencing after PCR amplification, and determined GATA2 expression by immunohistochemistry in a cohort of 180 CRC patients. Kaplan-Meier survival analysis and Cox proportional hazard regression were used to analyze the association between the GATA2 rs2335052 genotypes and the clinical outcome of CRC.

Results: We found that there was no significant correlation between the rs2335052 genotypes and the expression of GATA2. However, the Kaplan-Meier survival analysis suggested that the carriers of the A-allele of SNP rs2335052 were significantly associated with increased risk of recurrence and reduced disease-free survival (DFS), compared with those carrying the variant genotype of GG in rs2335052 (P = 0.021). Moreover, univariate and multivariate Cox regression analyses revealed that GATA2 SNP rs2335052 was an independent risk factor for the DFS of CRC patients.

Conclusion: Our results demonstrated that GATA2 SNP rs2335052 is an independent predictor for prognosis of CRC patients. This raised the possibility that SNP rs2335052 may serve as a potential indicator for predicting recurrence of CRC after curative colectomy.

No MeSH data available.


Related in: MedlinePlus