Limits...
Leptospermum flavescens Constituent-LF1 Causes Cell Death through the Induction of Cell Cycle Arrest and Apoptosis in Human Lung Carcinoma Cells.

Navanesan S, Abdul Wahab N, Manickam S, Sim KS - PLoS ONE (2015)

Bottom Line: LF1 showed the greatest cytotoxic effect against both cell lines with IC50 values of 7.12 ± 0.07 and 9.62 ± 0.50 μg/ml respectively.Blockage of cell cycle progression was also observed in LF1-treated cells.Further studies are being conducted to isolate and identify the active compound as well to better understand the mechanism involved in inducing cell death.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.

ABSTRACT
Leptospermum flavescens Sm. (Myrtaceae), locally known as 'Senna makki' is a smallish tree that is widespread and recorded to naturally occur in the montane regions above 900 m a.s.l from Burma to Australia. Although the species is recorded to be used traditionally to treat various ailments, there is limited data on biological and chemical investigations of L. flavescens. The aim of the present study was to investigate and understand the ability of L. flavescens in inducing cell death in lung cancer cells. The cytotoxic potentials of the extraction yields (methanol, hexane, ethyl acetate and water extracts as wells as a semi pure fraction, LF1) were evaluated against two human non-small cell lung carcinoma cell lines (A549 and NCI-H1299) using the MTT assay. LF1 showed the greatest cytotoxic effect against both cell lines with IC50 values of 7.12 ± 0.07 and 9.62 ± 0.50 μg/ml respectively. LF1 treated cells showed a sub-G1 region in the cell cycle analysis and also caused the presence of apoptotic morphologies in cells stained with acridine orange and ethidium bromide. Treatment with LF1 manifested an apoptotic population in cells that were evaluated using the Annexin V/ propidium iodide assay. Increasing dosage of LF1 caused a rise in the presence of activated caspase-3 enzymes in treated cells. Blockage of cell cycle progression was also observed in LF1-treated cells. These findings suggest that LF1 induces apoptosis and cell cycle arrest in treated lung cancer cells. Further studies are being conducted to isolate and identify the active compound as well to better understand the mechanism involved in inducing cell death.

No MeSH data available.


Related in: MedlinePlus

Effects of LF1 on cell cycle distribution in A549 NCI-H1299 cells.A549 (A) and NCI-H1299 (B) were incubated in absence (control) and presence of LF1 at 5, 7.5 and 10 μg/ml for 24 hours. Summary of results indicate an increase in G0/G1 population with increasing dosages of LF1 used.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4546061&req=5

pone.0135995.g007: Effects of LF1 on cell cycle distribution in A549 NCI-H1299 cells.A549 (A) and NCI-H1299 (B) were incubated in absence (control) and presence of LF1 at 5, 7.5 and 10 μg/ml for 24 hours. Summary of results indicate an increase in G0/G1 population with increasing dosages of LF1 used.

Mentions: Blockage in the cell cycle as a result of treatment with LF1 was assessed by staining fixed and permeabilized cell with the PI dye. The DNA content which was in the G0/G1 phase gradually increased with the increasing exposure to LF1 on both A549 and NCI-H1299 cell lines. In general, a reverse was observe in the S and G2/M phase population of both treated cell lines, whereby the number of events recorded depleted with the escalating dosage of LF1 used (Fig 7). These observations were done with reference to the untreated controls and the differences with the control were statistically significant (p < 0.05) in most cases.


Leptospermum flavescens Constituent-LF1 Causes Cell Death through the Induction of Cell Cycle Arrest and Apoptosis in Human Lung Carcinoma Cells.

Navanesan S, Abdul Wahab N, Manickam S, Sim KS - PLoS ONE (2015)

Effects of LF1 on cell cycle distribution in A549 NCI-H1299 cells.A549 (A) and NCI-H1299 (B) were incubated in absence (control) and presence of LF1 at 5, 7.5 and 10 μg/ml for 24 hours. Summary of results indicate an increase in G0/G1 population with increasing dosages of LF1 used.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4546061&req=5

pone.0135995.g007: Effects of LF1 on cell cycle distribution in A549 NCI-H1299 cells.A549 (A) and NCI-H1299 (B) were incubated in absence (control) and presence of LF1 at 5, 7.5 and 10 μg/ml for 24 hours. Summary of results indicate an increase in G0/G1 population with increasing dosages of LF1 used.
Mentions: Blockage in the cell cycle as a result of treatment with LF1 was assessed by staining fixed and permeabilized cell with the PI dye. The DNA content which was in the G0/G1 phase gradually increased with the increasing exposure to LF1 on both A549 and NCI-H1299 cell lines. In general, a reverse was observe in the S and G2/M phase population of both treated cell lines, whereby the number of events recorded depleted with the escalating dosage of LF1 used (Fig 7). These observations were done with reference to the untreated controls and the differences with the control were statistically significant (p < 0.05) in most cases.

Bottom Line: LF1 showed the greatest cytotoxic effect against both cell lines with IC50 values of 7.12 ± 0.07 and 9.62 ± 0.50 μg/ml respectively.Blockage of cell cycle progression was also observed in LF1-treated cells.Further studies are being conducted to isolate and identify the active compound as well to better understand the mechanism involved in inducing cell death.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.

ABSTRACT
Leptospermum flavescens Sm. (Myrtaceae), locally known as 'Senna makki' is a smallish tree that is widespread and recorded to naturally occur in the montane regions above 900 m a.s.l from Burma to Australia. Although the species is recorded to be used traditionally to treat various ailments, there is limited data on biological and chemical investigations of L. flavescens. The aim of the present study was to investigate and understand the ability of L. flavescens in inducing cell death in lung cancer cells. The cytotoxic potentials of the extraction yields (methanol, hexane, ethyl acetate and water extracts as wells as a semi pure fraction, LF1) were evaluated against two human non-small cell lung carcinoma cell lines (A549 and NCI-H1299) using the MTT assay. LF1 showed the greatest cytotoxic effect against both cell lines with IC50 values of 7.12 ± 0.07 and 9.62 ± 0.50 μg/ml respectively. LF1 treated cells showed a sub-G1 region in the cell cycle analysis and also caused the presence of apoptotic morphologies in cells stained with acridine orange and ethidium bromide. Treatment with LF1 manifested an apoptotic population in cells that were evaluated using the Annexin V/ propidium iodide assay. Increasing dosage of LF1 caused a rise in the presence of activated caspase-3 enzymes in treated cells. Blockage of cell cycle progression was also observed in LF1-treated cells. These findings suggest that LF1 induces apoptosis and cell cycle arrest in treated lung cancer cells. Further studies are being conducted to isolate and identify the active compound as well to better understand the mechanism involved in inducing cell death.

No MeSH data available.


Related in: MedlinePlus