Limits...
Lecithin-based wet chemical precipitation of hydroxyapatite nanoparticles.

Michał W, Ewa D, Tomasz C - Colloid Polym Sci (2015)

Bottom Line: Size distribution for nanoparticles was measured by nanoparticle tracking analysis in NanoSight system.We discuss in details influence of lecithin concentration in reaction system on nHAp-PC morphology, as well as on size distributions and suspendability of nanoparticles.Difference in surfactant amounts results in changes in particles morphology and their average size.

View Article: PubMed Central - PubMed

Affiliation: BioMedical Engineering Laboratory, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warszawa, Poland.

ABSTRACT

Hydroxyapatite Ca10(PO4)6(OH)2 nanoparticles have been successfully synthesized by the wet chemical precipitation method at 60 °C in the presence of biocompatible natural surfactant-lecithin. The composition and morphology of nanoparticles of hydroxyapatite synthesized with lecithin (nHAp-PC) was studied by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Size distribution for nanoparticles was measured by nanoparticle tracking analysis in NanoSight system. We discuss in details influence of lecithin concentration in reaction system on nHAp-PC morphology, as well as on size distributions and suspendability of nanoparticles. Product exhibits crystalline structure and chemical composition of hydroxyapatite, with visible traces of lecithin. Difference in surfactant amounts results in changes in particles morphology and their average size.

No MeSH data available.


Related in: MedlinePlus

NanoSight size distribution, dominant particle morphology, and average particle size of lecithin-based wet chemical precipitation synthesized hydroxyapatite nanoparticles: a 0.30 % w/w lecithin, b 0.75 % w/w lecithin, c 1.50 % w/w lecithin, d 3.00 % w/w lecithin, e 9.00 % w/w lecithin
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4544500&req=5

Fig4: NanoSight size distribution, dominant particle morphology, and average particle size of lecithin-based wet chemical precipitation synthesized hydroxyapatite nanoparticles: a 0.30 % w/w lecithin, b 0.75 % w/w lecithin, c 1.50 % w/w lecithin, d 3.00 % w/w lecithin, e 9.00 % w/w lecithin

Mentions: Using nanoparticles tracking analysis in NanoSight system, we recorded size distributions for all synthesized nHAp-PC (Fig. 4). Since the particles synthesized in the system with lecithin amount 0.30, 0.75, and 1.50 % w/w are non-spherical in shape, the results of average size measurement (particles were treated by the system as sphere-equivalent in morphology) are only the estimation for these particles appearance in aqueous environment. Based on SEM images, we estimated the average sizes of the nanoplates in height and width on about 1 to 4 μm, and the length of nanorods produced with addition of 0.75 and 1.50 % w/w of lecithin on about 100 to 400 nm and about 200 to 1 μm, respectively. Comparing the results of SEM measurements with NanoSight analysis, we were able to confirm presence of small, heterogeneous in shape particles in nanoplate samples, where the plates with nanosized thickness were not measured by NTA software (plates were over 1 μm in size—over the limit of the system). In the case of nanorod samples, for nHAp-PC synthesized with 0.75 % w/w addition of lecithin, we obtained the bimodal size distribution, which suggest presence of nanorods measured by system simultaneously in both directions. In all previously mentioned instances, the particles in suspension were well separated. For particles synthesized with 3.00 and 9.00 % w/w addition of lecithin—spherical nanoparticles, the average size we obtained is comparable with results from SEM measurements. SEM images analysis results with dimensions of nHAp-PC synthesized with 3.00 % w/w of lecithin about 40 to 150 nm and dimensions of nHAp-PC synthesized with 9.00 % w/w of lecithin about 60 to 250 nm. However, the average diameter of those particles measured in NanoSight system is about 75 to 90 and 90 to 110 nm, respectively. The size distributions recorded for nanospheres of hydroxyapatite obtained in presence of lecithin show the portion of the biggest particles about 400 nm. Since the aggregates visible in the SEM image (Fig. 3f) are much bigger (even 1 μm in diameter), we postulate that nHAp-PC might form proper suspension of nanoparticles in aqueous media.Fig. 4


Lecithin-based wet chemical precipitation of hydroxyapatite nanoparticles.

Michał W, Ewa D, Tomasz C - Colloid Polym Sci (2015)

NanoSight size distribution, dominant particle morphology, and average particle size of lecithin-based wet chemical precipitation synthesized hydroxyapatite nanoparticles: a 0.30 % w/w lecithin, b 0.75 % w/w lecithin, c 1.50 % w/w lecithin, d 3.00 % w/w lecithin, e 9.00 % w/w lecithin
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544500&req=5

Fig4: NanoSight size distribution, dominant particle morphology, and average particle size of lecithin-based wet chemical precipitation synthesized hydroxyapatite nanoparticles: a 0.30 % w/w lecithin, b 0.75 % w/w lecithin, c 1.50 % w/w lecithin, d 3.00 % w/w lecithin, e 9.00 % w/w lecithin
Mentions: Using nanoparticles tracking analysis in NanoSight system, we recorded size distributions for all synthesized nHAp-PC (Fig. 4). Since the particles synthesized in the system with lecithin amount 0.30, 0.75, and 1.50 % w/w are non-spherical in shape, the results of average size measurement (particles were treated by the system as sphere-equivalent in morphology) are only the estimation for these particles appearance in aqueous environment. Based on SEM images, we estimated the average sizes of the nanoplates in height and width on about 1 to 4 μm, and the length of nanorods produced with addition of 0.75 and 1.50 % w/w of lecithin on about 100 to 400 nm and about 200 to 1 μm, respectively. Comparing the results of SEM measurements with NanoSight analysis, we were able to confirm presence of small, heterogeneous in shape particles in nanoplate samples, where the plates with nanosized thickness were not measured by NTA software (plates were over 1 μm in size—over the limit of the system). In the case of nanorod samples, for nHAp-PC synthesized with 0.75 % w/w addition of lecithin, we obtained the bimodal size distribution, which suggest presence of nanorods measured by system simultaneously in both directions. In all previously mentioned instances, the particles in suspension were well separated. For particles synthesized with 3.00 and 9.00 % w/w addition of lecithin—spherical nanoparticles, the average size we obtained is comparable with results from SEM measurements. SEM images analysis results with dimensions of nHAp-PC synthesized with 3.00 % w/w of lecithin about 40 to 150 nm and dimensions of nHAp-PC synthesized with 9.00 % w/w of lecithin about 60 to 250 nm. However, the average diameter of those particles measured in NanoSight system is about 75 to 90 and 90 to 110 nm, respectively. The size distributions recorded for nanospheres of hydroxyapatite obtained in presence of lecithin show the portion of the biggest particles about 400 nm. Since the aggregates visible in the SEM image (Fig. 3f) are much bigger (even 1 μm in diameter), we postulate that nHAp-PC might form proper suspension of nanoparticles in aqueous media.Fig. 4

Bottom Line: Size distribution for nanoparticles was measured by nanoparticle tracking analysis in NanoSight system.We discuss in details influence of lecithin concentration in reaction system on nHAp-PC morphology, as well as on size distributions and suspendability of nanoparticles.Difference in surfactant amounts results in changes in particles morphology and their average size.

View Article: PubMed Central - PubMed

Affiliation: BioMedical Engineering Laboratory, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warszawa, Poland.

ABSTRACT

Hydroxyapatite Ca10(PO4)6(OH)2 nanoparticles have been successfully synthesized by the wet chemical precipitation method at 60 °C in the presence of biocompatible natural surfactant-lecithin. The composition and morphology of nanoparticles of hydroxyapatite synthesized with lecithin (nHAp-PC) was studied by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Size distribution for nanoparticles was measured by nanoparticle tracking analysis in NanoSight system. We discuss in details influence of lecithin concentration in reaction system on nHAp-PC morphology, as well as on size distributions and suspendability of nanoparticles. Product exhibits crystalline structure and chemical composition of hydroxyapatite, with visible traces of lecithin. Difference in surfactant amounts results in changes in particles morphology and their average size.

No MeSH data available.


Related in: MedlinePlus