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Identification and mapping of quantitative trait loci (QTL) conferring resistance to Fusarium graminearum from soybean PI 567301B.

Acharya B, Lee S, Rouf Mian MA, Jun TH, McHale LK, Michel AP, Dorrance AE - Theor. Appl. Genet. (2015)

Bottom Line: The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence.Based on previous screens, PI 567301B is susceptible to SCN.Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA.

ABSTRACT

Key message: A major novel QTL was identified in a recombinant inbred line population derived from a cross of 'Wyandot' × PI 567301B for Fusarium graminearum, a seed and seedling pathogen of soybean. Fusarium graminearum is now recognized as a primary pathogen of soybean, causing root, seed rot and seedling damping-off in North America. In a preliminary screen, 'Wyandot' and PI 567301B were identified with medium and high levels of partial resistance to F. graminearum, respectively. The objective of this study was to characterise resistance towards F. graminearum using 184 recombinant inbred lines (RILs) derived from a cross of 'Wyandot' × PI 567301B. The parents and the RILs of the mapping population were evaluated for resistance towards F. graminearum using the rolled towel assay in a randomized incomplete block design. A genetic map was constructed from 2545 SNP markers and 2 SSR markers by composite interval mapping. One major and one minor QTL were identified on chromosomes 8 and 6, respectively, which explained 38.5 and 8.1 % of the phenotypic variance. The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence. Annotation of this region indicates that there are 39 genes including the Rhg4 locus for soybean cyst nematode (SCN) resistance. Based on previous screens, PI 567301B is susceptible to SCN. Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum.

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Related in: MedlinePlus

Graphical presentation of quantitative trait loci (QTL) for resistance to Fusarium graminearum identified on chromosome a 8 and b 6 in the ‘Wyandot’ × PI 567301B population by genome-wide LOD threshold 3.4 (a hatched line in LOD plots). The 1- and 2-LOD intervals are presented by a black bar and solid lines between the chromosome and the LOD plot for each QTL. The enlarged image was presented side by side to highlight approximate 20 cM region around LOD peak of each QTL
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Fig2: Graphical presentation of quantitative trait loci (QTL) for resistance to Fusarium graminearum identified on chromosome a 8 and b 6 in the ‘Wyandot’ × PI 567301B population by genome-wide LOD threshold 3.4 (a hatched line in LOD plots). The 1- and 2-LOD intervals are presented by a black bar and solid lines between the chromosome and the LOD plot for each QTL. The enlarged image was presented side by side to highlight approximate 20 cM region around LOD peak of each QTL

Mentions: Two QTL conferring resistance to F. graminearum were identified in the Wyandot × PI 567301B population by CIM, one major QTL on chromosome 8 and one minor QTL on chromosome 6 (Table 2). Both QTL were highly significant with respective LOD scores of 23.9 and 6.3 compared to the genome-wide LOD threshold of 3.4 calculated by a 1000-permutation test. The major QTL on chromosome 8 was flanked by markers Sat_157 and ss715602786 and accounted for 38.5 % of the phenotypic variance (Table 2; Fig. 2a). A minor QTL, which explained 8.1 % of the phenotypic variance, was closely linked to marker ss715593768 (Glyma.Wm82.a2, Gm06 at 17672411 bp) on chromosome 6 (Table 2; Fig. 2b). The resistance alleles for both QTL were contributed by PI 567301B (Table 2).Table 2


Identification and mapping of quantitative trait loci (QTL) conferring resistance to Fusarium graminearum from soybean PI 567301B.

Acharya B, Lee S, Rouf Mian MA, Jun TH, McHale LK, Michel AP, Dorrance AE - Theor. Appl. Genet. (2015)

Graphical presentation of quantitative trait loci (QTL) for resistance to Fusarium graminearum identified on chromosome a 8 and b 6 in the ‘Wyandot’ × PI 567301B population by genome-wide LOD threshold 3.4 (a hatched line in LOD plots). The 1- and 2-LOD intervals are presented by a black bar and solid lines between the chromosome and the LOD plot for each QTL. The enlarged image was presented side by side to highlight approximate 20 cM region around LOD peak of each QTL
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544499&req=5

Fig2: Graphical presentation of quantitative trait loci (QTL) for resistance to Fusarium graminearum identified on chromosome a 8 and b 6 in the ‘Wyandot’ × PI 567301B population by genome-wide LOD threshold 3.4 (a hatched line in LOD plots). The 1- and 2-LOD intervals are presented by a black bar and solid lines between the chromosome and the LOD plot for each QTL. The enlarged image was presented side by side to highlight approximate 20 cM region around LOD peak of each QTL
Mentions: Two QTL conferring resistance to F. graminearum were identified in the Wyandot × PI 567301B population by CIM, one major QTL on chromosome 8 and one minor QTL on chromosome 6 (Table 2). Both QTL were highly significant with respective LOD scores of 23.9 and 6.3 compared to the genome-wide LOD threshold of 3.4 calculated by a 1000-permutation test. The major QTL on chromosome 8 was flanked by markers Sat_157 and ss715602786 and accounted for 38.5 % of the phenotypic variance (Table 2; Fig. 2a). A minor QTL, which explained 8.1 % of the phenotypic variance, was closely linked to marker ss715593768 (Glyma.Wm82.a2, Gm06 at 17672411 bp) on chromosome 6 (Table 2; Fig. 2b). The resistance alleles for both QTL were contributed by PI 567301B (Table 2).Table 2

Bottom Line: The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence.Based on previous screens, PI 567301B is susceptible to SCN.Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA.

ABSTRACT

Key message: A major novel QTL was identified in a recombinant inbred line population derived from a cross of 'Wyandot' × PI 567301B for Fusarium graminearum, a seed and seedling pathogen of soybean. Fusarium graminearum is now recognized as a primary pathogen of soybean, causing root, seed rot and seedling damping-off in North America. In a preliminary screen, 'Wyandot' and PI 567301B were identified with medium and high levels of partial resistance to F. graminearum, respectively. The objective of this study was to characterise resistance towards F. graminearum using 184 recombinant inbred lines (RILs) derived from a cross of 'Wyandot' × PI 567301B. The parents and the RILs of the mapping population were evaluated for resistance towards F. graminearum using the rolled towel assay in a randomized incomplete block design. A genetic map was constructed from 2545 SNP markers and 2 SSR markers by composite interval mapping. One major and one minor QTL were identified on chromosomes 8 and 6, respectively, which explained 38.5 and 8.1 % of the phenotypic variance. The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence. Annotation of this region indicates that there are 39 genes including the Rhg4 locus for soybean cyst nematode (SCN) resistance. Based on previous screens, PI 567301B is susceptible to SCN. Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum.

Show MeSH
Related in: MedlinePlus