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Identification and mapping of quantitative trait loci (QTL) conferring resistance to Fusarium graminearum from soybean PI 567301B.

Acharya B, Lee S, Rouf Mian MA, Jun TH, McHale LK, Michel AP, Dorrance AE - Theor. Appl. Genet. (2015)

Bottom Line: The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence.Based on previous screens, PI 567301B is susceptible to SCN.Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA.

ABSTRACT

Key message: A major novel QTL was identified in a recombinant inbred line population derived from a cross of 'Wyandot' × PI 567301B for Fusarium graminearum, a seed and seedling pathogen of soybean. Fusarium graminearum is now recognized as a primary pathogen of soybean, causing root, seed rot and seedling damping-off in North America. In a preliminary screen, 'Wyandot' and PI 567301B were identified with medium and high levels of partial resistance to F. graminearum, respectively. The objective of this study was to characterise resistance towards F. graminearum using 184 recombinant inbred lines (RILs) derived from a cross of 'Wyandot' × PI 567301B. The parents and the RILs of the mapping population were evaluated for resistance towards F. graminearum using the rolled towel assay in a randomized incomplete block design. A genetic map was constructed from 2545 SNP markers and 2 SSR markers by composite interval mapping. One major and one minor QTL were identified on chromosomes 8 and 6, respectively, which explained 38.5 and 8.1 % of the phenotypic variance. The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence. Annotation of this region indicates that there are 39 genes including the Rhg4 locus for soybean cyst nematode (SCN) resistance. Based on previous screens, PI 567301B is susceptible to SCN. Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum.

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Frequency distribution of BLUP values for disease severity index of F7:10 recombinant inbred lines (RILs) population derived from the cross of ‘Wyandot’ × PI 567301B. Estimates of two parents and checks are indicated by arrows. A lower BLUP value means higher level of resistance to F. graminearum
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Fig1: Frequency distribution of BLUP values for disease severity index of F7:10 recombinant inbred lines (RILs) population derived from the cross of ‘Wyandot’ × PI 567301B. Estimates of two parents and checks are indicated by arrows. A lower BLUP value means higher level of resistance to F. graminearum

Mentions: The mean DSI was significantly different among the two parents and the checks indicating that PI 567301B had a high level of resistance and Wyandot had a moderate level of resistance towards F. graminearum (Table 1). The mean DSI and root rot scores of parents and checks were significantly different from one another (P < 0.0001; Fisher’s protected least significant difference test) (Table 1). The mean DSI of individual RILs ranged from 0.8 to 63.7 %, and the overall mean DSI of all the RILs was 32.6 %. Both Wyandot and PI 567301B had BLUP values less than Conrad, a moderately resistant cultivar, indicating that these parents had a higher level of resistance to F. graminearum than Conrad and that this was a moderately resistant by resistant cross (Fig. 1).Table 1


Identification and mapping of quantitative trait loci (QTL) conferring resistance to Fusarium graminearum from soybean PI 567301B.

Acharya B, Lee S, Rouf Mian MA, Jun TH, McHale LK, Michel AP, Dorrance AE - Theor. Appl. Genet. (2015)

Frequency distribution of BLUP values for disease severity index of F7:10 recombinant inbred lines (RILs) population derived from the cross of ‘Wyandot’ × PI 567301B. Estimates of two parents and checks are indicated by arrows. A lower BLUP value means higher level of resistance to F. graminearum
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544499&req=5

Fig1: Frequency distribution of BLUP values for disease severity index of F7:10 recombinant inbred lines (RILs) population derived from the cross of ‘Wyandot’ × PI 567301B. Estimates of two parents and checks are indicated by arrows. A lower BLUP value means higher level of resistance to F. graminearum
Mentions: The mean DSI was significantly different among the two parents and the checks indicating that PI 567301B had a high level of resistance and Wyandot had a moderate level of resistance towards F. graminearum (Table 1). The mean DSI and root rot scores of parents and checks were significantly different from one another (P < 0.0001; Fisher’s protected least significant difference test) (Table 1). The mean DSI of individual RILs ranged from 0.8 to 63.7 %, and the overall mean DSI of all the RILs was 32.6 %. Both Wyandot and PI 567301B had BLUP values less than Conrad, a moderately resistant cultivar, indicating that these parents had a higher level of resistance to F. graminearum than Conrad and that this was a moderately resistant by resistant cross (Fig. 1).Table 1

Bottom Line: The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence.Based on previous screens, PI 567301B is susceptible to SCN.Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA.

ABSTRACT

Key message: A major novel QTL was identified in a recombinant inbred line population derived from a cross of 'Wyandot' × PI 567301B for Fusarium graminearum, a seed and seedling pathogen of soybean. Fusarium graminearum is now recognized as a primary pathogen of soybean, causing root, seed rot and seedling damping-off in North America. In a preliminary screen, 'Wyandot' and PI 567301B were identified with medium and high levels of partial resistance to F. graminearum, respectively. The objective of this study was to characterise resistance towards F. graminearum using 184 recombinant inbred lines (RILs) derived from a cross of 'Wyandot' × PI 567301B. The parents and the RILs of the mapping population were evaluated for resistance towards F. graminearum using the rolled towel assay in a randomized incomplete block design. A genetic map was constructed from 2545 SNP markers and 2 SSR markers by composite interval mapping. One major and one minor QTL were identified on chromosomes 8 and 6, respectively, which explained 38.5 and 8.1 % of the phenotypic variance. The major QTL on chromosome 8 was mapped to a 300 kb size genomic region of the Williams 82 sequence. Annotation of this region indicates that there are 39 genes including the Rhg4 locus for soybean cyst nematode (SCN) resistance. Based on previous screens, PI 567301B is susceptible to SCN. Fine mapping of this locus will assist in cloning these candidate genes as well as identifying DNA markers flanking the QTL that can be used in marker-assisted breeding to develop cultivars with high levels of resistance to F. graminearum.

Show MeSH
Related in: MedlinePlus