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An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species.

Li GR, Ji W, Wang G, Zhang JX, Wang YJ - In Vitro Cell. Dev. Biol., Plant (2013)

Bottom Line: The results indicated that Beichun and Shuangyou were the best male parents.The best sampling time for ovule inoculation differed among the female parents.Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi 712100 People's Republic of China ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China.

ABSTRACT

A highly efficient technique of embryo rescue is critical when using stenospermocarpic Vitis vinifera cultivars (female parents) to breed novel, disease-resistant, seedless grape cultivars by hybridizing with wild Chinese Vitis species (male parents) having many disease-resistance alleles. The effects of various factors on the improvement of embryo formation, germination, and plantlet development for seven hybrid combinations were studied. The results indicated that Beichun and Shuangyou were the best male parents. The best sampling time for ovule inoculation differed among the female parents. When hybrid ovules were cultured on a double-phase medium with five different solid medium types, percent embryo formation was highest (11.3-28.3%) on a modified MM3 medium. Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar. In the absence of other amino acids, the addition of proline significantly increased embryo formation (36.1%), embryo germination (64.6%), and plantlet development (90.5%). A highly efficient protocol has been developed for hybrid embryo rescue from seedless V. vinifera grapes × wild Chinese Vitis species that results in a significant improvement in breeding efficiency for new disease-resistant seedless grapes.

No MeSH data available.


Related in: MedlinePlus

The quadratic regression equations on the sampling time of Ruby Seedless and percent embryo formation through Excel software. r = 0.8173, P ≤ .05. Approximately 65 d was the optimal delay with embryo rescue being limited by immaturity before this time, and by natural abortion afterwards.
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Fig7: The quadratic regression equations on the sampling time of Ruby Seedless and percent embryo formation through Excel software. r = 0.8173, P ≤ .05. Approximately 65 d was the optimal delay with embryo rescue being limited by immaturity before this time, and by natural abortion afterwards.

Mentions: Sampling time (the embryo rescue time) had a significant effect on embryo rescue efficiency. Embryos are hard to save if sampling time is too early, but they will abort if sampling time is too late (Bin et al. 1991). Therefore, for seedless grape breeding using embryo-rescue techniques, it is critical to determine the optimal sampling time for each cultivar under investigation. This should be identified on the basis of the number of embryos that develop from cultured ovules (Pommer et al. 1995; Midani et al. 2002; Liu et al. 2003; Li et al. 2004). Here, we observed that the optimal sampling time depended on the elapsed time (days) between pollination and the maximum percent embryo formation. The optimal sampling time differed among the seedless grapes examined here as female parents. The sampling time was negatively correlated with percent embryo formation of seedless grapes. The sampling time was significantly and negatively correlated with percent embryo formation of Thompson Seedless (r = 0.8883, P ≤ .05; Fig. 4), Flame Seedless (r = 0.9050, P ≤ .05; Fig. 5), Crimson Seedless (r = 0.9487, P ≤ .05; Fig. 6), and Ruby Seedless (r = 0.8173, P ≤ .05; Fig. 7). The highest ovule formation percentage of Thompson Seedless was 37.5%. Approximately 37 d was the optimal delay with embryo rescue being limited by immaturity before this time, and by natural abortion afterwards. In Flame Seedless, Crimson Seedless, and Ruby Seedless the same values were 35.8, 35.0, and 45.7% occurring at 45, 60, and 65 d, respectively.Figure 4.


An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species.

Li GR, Ji W, Wang G, Zhang JX, Wang YJ - In Vitro Cell. Dev. Biol., Plant (2013)

The quadratic regression equations on the sampling time of Ruby Seedless and percent embryo formation through Excel software. r = 0.8173, P ≤ .05. Approximately 65 d was the optimal delay with embryo rescue being limited by immaturity before this time, and by natural abortion afterwards.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544466&req=5

Fig7: The quadratic regression equations on the sampling time of Ruby Seedless and percent embryo formation through Excel software. r = 0.8173, P ≤ .05. Approximately 65 d was the optimal delay with embryo rescue being limited by immaturity before this time, and by natural abortion afterwards.
Mentions: Sampling time (the embryo rescue time) had a significant effect on embryo rescue efficiency. Embryos are hard to save if sampling time is too early, but they will abort if sampling time is too late (Bin et al. 1991). Therefore, for seedless grape breeding using embryo-rescue techniques, it is critical to determine the optimal sampling time for each cultivar under investigation. This should be identified on the basis of the number of embryos that develop from cultured ovules (Pommer et al. 1995; Midani et al. 2002; Liu et al. 2003; Li et al. 2004). Here, we observed that the optimal sampling time depended on the elapsed time (days) between pollination and the maximum percent embryo formation. The optimal sampling time differed among the seedless grapes examined here as female parents. The sampling time was negatively correlated with percent embryo formation of seedless grapes. The sampling time was significantly and negatively correlated with percent embryo formation of Thompson Seedless (r = 0.8883, P ≤ .05; Fig. 4), Flame Seedless (r = 0.9050, P ≤ .05; Fig. 5), Crimson Seedless (r = 0.9487, P ≤ .05; Fig. 6), and Ruby Seedless (r = 0.8173, P ≤ .05; Fig. 7). The highest ovule formation percentage of Thompson Seedless was 37.5%. Approximately 37 d was the optimal delay with embryo rescue being limited by immaturity before this time, and by natural abortion afterwards. In Flame Seedless, Crimson Seedless, and Ruby Seedless the same values were 35.8, 35.0, and 45.7% occurring at 45, 60, and 65 d, respectively.Figure 4.

Bottom Line: The results indicated that Beichun and Shuangyou were the best male parents.The best sampling time for ovule inoculation differed among the female parents.Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi 712100 People's Republic of China ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China.

ABSTRACT

A highly efficient technique of embryo rescue is critical when using stenospermocarpic Vitis vinifera cultivars (female parents) to breed novel, disease-resistant, seedless grape cultivars by hybridizing with wild Chinese Vitis species (male parents) having many disease-resistance alleles. The effects of various factors on the improvement of embryo formation, germination, and plantlet development for seven hybrid combinations were studied. The results indicated that Beichun and Shuangyou were the best male parents. The best sampling time for ovule inoculation differed among the female parents. When hybrid ovules were cultured on a double-phase medium with five different solid medium types, percent embryo formation was highest (11.3-28.3%) on a modified MM3 medium. Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar. In the absence of other amino acids, the addition of proline significantly increased embryo formation (36.1%), embryo germination (64.6%), and plantlet development (90.5%). A highly efficient protocol has been developed for hybrid embryo rescue from seedless V. vinifera grapes × wild Chinese Vitis species that results in a significant improvement in breeding efficiency for new disease-resistant seedless grapes.

No MeSH data available.


Related in: MedlinePlus