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An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species.

Li GR, Ji W, Wang G, Zhang JX, Wang YJ - In Vitro Cell. Dev. Biol., Plant (2013)

Bottom Line: The results indicated that Beichun and Shuangyou were the best male parents.The best sampling time for ovule inoculation differed among the female parents.Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi 712100 People's Republic of China ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China.

ABSTRACT

A highly efficient technique of embryo rescue is critical when using stenospermocarpic Vitis vinifera cultivars (female parents) to breed novel, disease-resistant, seedless grape cultivars by hybridizing with wild Chinese Vitis species (male parents) having many disease-resistance alleles. The effects of various factors on the improvement of embryo formation, germination, and plantlet development for seven hybrid combinations were studied. The results indicated that Beichun and Shuangyou were the best male parents. The best sampling time for ovule inoculation differed among the female parents. When hybrid ovules were cultured on a double-phase medium with five different solid medium types, percent embryo formation was highest (11.3-28.3%) on a modified MM3 medium. Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar. In the absence of other amino acids, the addition of proline significantly increased embryo formation (36.1%), embryo germination (64.6%), and plantlet development (90.5%). A highly efficient protocol has been developed for hybrid embryo rescue from seedless V. vinifera grapes × wild Chinese Vitis species that results in a significant improvement in breeding efficiency for new disease-resistant seedless grapes.

No MeSH data available.


Related in: MedlinePlus

Pollen tube lengths (millimeters ± SE after 24 h) for seven cultivars cultured on pollen medium. Significant (P ≤ .05) differences in tube lengths were not observed between Red Globe, Beichun, and Shuangyou. The other five cultivars were significantly different.
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Fig3: Pollen tube lengths (millimeters ± SE after 24 h) for seven cultivars cultured on pollen medium. Significant (P ≤ .05) differences in tube lengths were not observed between Red Globe, Beichun, and Shuangyou. The other five cultivars were significantly different.

Mentions: Similarly, a one-way ANOVA analysis for pollen tube length showed that the seven cultivars had different pollen tube lengths (P ≤ .05) ranging from 0.22 to 1.15 mm (Fig. 3). The pollen tube lengths of Red Globe were the longest (1.15 mm). Pollen tube lengths of five of the cultivars (0.85 mm for Flame Seedless, 0.64 mm for Tangwei and Xuefeng, 0.22 mm for Yanshan) were significantly different (P ≤ .05). There were no significant differences (P ≤ .05) between the pollen tube lengths of Beichun (0.97 ± 0.06 mm), Shuangyou (0.88 ± 0.06 mm), and Red Globe (1.15 ± 0.06 mm). Kelen and Demirtas (2003) also drew similar conclusions, namely that pollen viability, germinability, and tube growth differed significantly among grape cultivars. These authors also studied the fertilization biology of eight grape cultivars including berry diameter and length, berry weight, seed numbers, and seed weight. In the future, we will investigate the fertilization biology of our seven crosses and investigate further the relationship between pollen production and fruit set. Our research examined the pollen viability of seven seedless cultivars. Of the wild Chinese Vitis species or cultivars, Beichun and Shuangyou are considered to be the best male parents for hybridization with seedless V. vinifera grape cultivars.Figure 3.


An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species.

Li GR, Ji W, Wang G, Zhang JX, Wang YJ - In Vitro Cell. Dev. Biol., Plant (2013)

Pollen tube lengths (millimeters ± SE after 24 h) for seven cultivars cultured on pollen medium. Significant (P ≤ .05) differences in tube lengths were not observed between Red Globe, Beichun, and Shuangyou. The other five cultivars were significantly different.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544466&req=5

Fig3: Pollen tube lengths (millimeters ± SE after 24 h) for seven cultivars cultured on pollen medium. Significant (P ≤ .05) differences in tube lengths were not observed between Red Globe, Beichun, and Shuangyou. The other five cultivars were significantly different.
Mentions: Similarly, a one-way ANOVA analysis for pollen tube length showed that the seven cultivars had different pollen tube lengths (P ≤ .05) ranging from 0.22 to 1.15 mm (Fig. 3). The pollen tube lengths of Red Globe were the longest (1.15 mm). Pollen tube lengths of five of the cultivars (0.85 mm for Flame Seedless, 0.64 mm for Tangwei and Xuefeng, 0.22 mm for Yanshan) were significantly different (P ≤ .05). There were no significant differences (P ≤ .05) between the pollen tube lengths of Beichun (0.97 ± 0.06 mm), Shuangyou (0.88 ± 0.06 mm), and Red Globe (1.15 ± 0.06 mm). Kelen and Demirtas (2003) also drew similar conclusions, namely that pollen viability, germinability, and tube growth differed significantly among grape cultivars. These authors also studied the fertilization biology of eight grape cultivars including berry diameter and length, berry weight, seed numbers, and seed weight. In the future, we will investigate the fertilization biology of our seven crosses and investigate further the relationship between pollen production and fruit set. Our research examined the pollen viability of seven seedless cultivars. Of the wild Chinese Vitis species or cultivars, Beichun and Shuangyou are considered to be the best male parents for hybridization with seedless V. vinifera grape cultivars.Figure 3.

Bottom Line: The results indicated that Beichun and Shuangyou were the best male parents.The best sampling time for ovule inoculation differed among the female parents.Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi 712100 People's Republic of China ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China.

ABSTRACT

A highly efficient technique of embryo rescue is critical when using stenospermocarpic Vitis vinifera cultivars (female parents) to breed novel, disease-resistant, seedless grape cultivars by hybridizing with wild Chinese Vitis species (male parents) having many disease-resistance alleles. The effects of various factors on the improvement of embryo formation, germination, and plantlet development for seven hybrid combinations were studied. The results indicated that Beichun and Shuangyou were the best male parents. The best sampling time for ovule inoculation differed among the female parents. When hybrid ovules were cultured on a double-phase medium with five different solid medium types, percent embryo formation was highest (11.3-28.3%) on a modified MM3 medium. Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar. In the absence of other amino acids, the addition of proline significantly increased embryo formation (36.1%), embryo germination (64.6%), and plantlet development (90.5%). A highly efficient protocol has been developed for hybrid embryo rescue from seedless V. vinifera grapes × wild Chinese Vitis species that results in a significant improvement in breeding efficiency for new disease-resistant seedless grapes.

No MeSH data available.


Related in: MedlinePlus