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An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species.

Li GR, Ji W, Wang G, Zhang JX, Wang YJ - In Vitro Cell. Dev. Biol., Plant (2013)

Bottom Line: The results indicated that Beichun and Shuangyou were the best male parents.The best sampling time for ovule inoculation differed among the female parents.Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi 712100 People's Republic of China ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China.

ABSTRACT

A highly efficient technique of embryo rescue is critical when using stenospermocarpic Vitis vinifera cultivars (female parents) to breed novel, disease-resistant, seedless grape cultivars by hybridizing with wild Chinese Vitis species (male parents) having many disease-resistance alleles. The effects of various factors on the improvement of embryo formation, germination, and plantlet development for seven hybrid combinations were studied. The results indicated that Beichun and Shuangyou were the best male parents. The best sampling time for ovule inoculation differed among the female parents. When hybrid ovules were cultured on a double-phase medium with five different solid medium types, percent embryo formation was highest (11.3-28.3%) on a modified MM3 medium. Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar. In the absence of other amino acids, the addition of proline significantly increased embryo formation (36.1%), embryo germination (64.6%), and plantlet development (90.5%). A highly efficient protocol has been developed for hybrid embryo rescue from seedless V. vinifera grapes × wild Chinese Vitis species that results in a significant improvement in breeding efficiency for new disease-resistant seedless grapes.

No MeSH data available.


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Pollen germination rate (percentage ± SE after 24 h) of seven cultivars cultured on pollen medium. Highest percent germination was observed for Red Globe. There were no significant differences (P ≤ .05) between Red Globe, Beichun, Shuangyou, and Xuefeng.
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Fig2: Pollen germination rate (percentage ± SE after 24 h) of seven cultivars cultured on pollen medium. Highest percent germination was observed for Red Globe. There were no significant differences (P ≤ .05) between Red Globe, Beichun, Shuangyou, and Xuefeng.

Mentions: Pollination and fertilization are necessary for fruit set in crosses of seedless V. vinifera × wild Chinese Vitis species. The essential conditions for fertilization are perfect development of the flowering organs in the V. vinifera female parent and the production of pollen of high germinability and viability by the wild Chinese Vitis species male parent. Except under special conditions, there is a linear relationship between pollen germinability and viability in many fruit species (Dafni and Firmage 2000). Pollen germinability is also related to cultivar, nutritional conditions, and environmental factors (Parton et al. 2002; Watrud et al. 2011). Pollen samples from seven of the cultivars used in our breeding program were analyzed in vitro and showed significant differences among the cultivars in germination and growth (Table 1). A one-way ANOVA for pollen germination shows that the factor ‘cultivar’ had a significant effect on pollen germination (P ≤ .05; Fig. 2). The germination percentage of Red Globe pollen was highest (41.3%). No significant differences in pollen germination were observed between Beichun (38.2 ± 1.6%), Shuangyou (36.4 ± 0.9%), Xuefeng (34.3 ± 1.5%), and Red Globe (41.3 ± 2.8%). Yanshan had a pollen germination percentage of only 8.3 ± 1.2%.Figure 2.


An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species.

Li GR, Ji W, Wang G, Zhang JX, Wang YJ - In Vitro Cell. Dev. Biol., Plant (2013)

Pollen germination rate (percentage ± SE after 24 h) of seven cultivars cultured on pollen medium. Highest percent germination was observed for Red Globe. There were no significant differences (P ≤ .05) between Red Globe, Beichun, Shuangyou, and Xuefeng.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4544466&req=5

Fig2: Pollen germination rate (percentage ± SE after 24 h) of seven cultivars cultured on pollen medium. Highest percent germination was observed for Red Globe. There were no significant differences (P ≤ .05) between Red Globe, Beichun, Shuangyou, and Xuefeng.
Mentions: Pollination and fertilization are necessary for fruit set in crosses of seedless V. vinifera × wild Chinese Vitis species. The essential conditions for fertilization are perfect development of the flowering organs in the V. vinifera female parent and the production of pollen of high germinability and viability by the wild Chinese Vitis species male parent. Except under special conditions, there is a linear relationship between pollen germinability and viability in many fruit species (Dafni and Firmage 2000). Pollen germinability is also related to cultivar, nutritional conditions, and environmental factors (Parton et al. 2002; Watrud et al. 2011). Pollen samples from seven of the cultivars used in our breeding program were analyzed in vitro and showed significant differences among the cultivars in germination and growth (Table 1). A one-way ANOVA for pollen germination shows that the factor ‘cultivar’ had a significant effect on pollen germination (P ≤ .05; Fig. 2). The germination percentage of Red Globe pollen was highest (41.3%). No significant differences in pollen germination were observed between Beichun (38.2 ± 1.6%), Shuangyou (36.4 ± 0.9%), Xuefeng (34.3 ± 1.5%), and Red Globe (41.3 ± 2.8%). Yanshan had a pollen germination percentage of only 8.3 ± 1.2%.Figure 2.

Bottom Line: The results indicated that Beichun and Shuangyou were the best male parents.The best sampling time for ovule inoculation differed among the female parents.Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi 712100 People's Republic of China ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China.

ABSTRACT

A highly efficient technique of embryo rescue is critical when using stenospermocarpic Vitis vinifera cultivars (female parents) to breed novel, disease-resistant, seedless grape cultivars by hybridizing with wild Chinese Vitis species (male parents) having many disease-resistance alleles. The effects of various factors on the improvement of embryo formation, germination, and plantlet development for seven hybrid combinations were studied. The results indicated that Beichun and Shuangyou were the best male parents. The best sampling time for ovule inoculation differed among the female parents. When hybrid ovules were cultured on a double-phase medium with five different solid medium types, percent embryo formation was highest (11.3-28.3%) on a modified MM3 medium. Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar. In the absence of other amino acids, the addition of proline significantly increased embryo formation (36.1%), embryo germination (64.6%), and plantlet development (90.5%). A highly efficient protocol has been developed for hybrid embryo rescue from seedless V. vinifera grapes × wild Chinese Vitis species that results in a significant improvement in breeding efficiency for new disease-resistant seedless grapes.

No MeSH data available.


Related in: MedlinePlus