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An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species.

Li GR, Ji W, Wang G, Zhang JX, Wang YJ - In Vitro Cell. Dev. Biol., Plant (2013)

Bottom Line: The results indicated that Beichun and Shuangyou were the best male parents.The best sampling time for ovule inoculation differed among the female parents.Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi 712100 People's Republic of China ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China.

ABSTRACT

A highly efficient technique of embryo rescue is critical when using stenospermocarpic Vitis vinifera cultivars (female parents) to breed novel, disease-resistant, seedless grape cultivars by hybridizing with wild Chinese Vitis species (male parents) having many disease-resistance alleles. The effects of various factors on the improvement of embryo formation, germination, and plantlet development for seven hybrid combinations were studied. The results indicated that Beichun and Shuangyou were the best male parents. The best sampling time for ovule inoculation differed among the female parents. When hybrid ovules were cultured on a double-phase medium with five different solid medium types, percent embryo formation was highest (11.3-28.3%) on a modified MM3 medium. Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar. In the absence of other amino acids, the addition of proline significantly increased embryo formation (36.1%), embryo germination (64.6%), and plantlet development (90.5%). A highly efficient protocol has been developed for hybrid embryo rescue from seedless V. vinifera grapes × wild Chinese Vitis species that results in a significant improvement in breeding efficiency for new disease-resistant seedless grapes.

No MeSH data available.


Related in: MedlinePlus

Embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species: (a) Inflorescence of female parent at anthesis and (b) at fruit set. (c–d) Ovules cultured in modified MM3 medium, and germinated embryos excised after 8 wk culture. (e) Whole plantlets developed from germinated embryos. (f) Tube plantlets transplanted to pots and sealed with transparent plastic cups. (g) Acclimation in a greenhouse. (h) Plants established in the field.
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Related In: Results  -  Collection


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Fig1: Embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species: (a) Inflorescence of female parent at anthesis and (b) at fruit set. (c–d) Ovules cultured in modified MM3 medium, and germinated embryos excised after 8 wk culture. (e) Whole plantlets developed from germinated embryos. (f) Tube plantlets transplanted to pots and sealed with transparent plastic cups. (g) Acclimation in a greenhouse. (h) Plants established in the field.

Mentions: Artificial pollination employed a soft brush loaded with dry pollen and was performed when the stigma mucus of the emasculated female parents was highest (Fig. 1a, b). Pollination was repeated three times on each of three consecutive days, followed by bagging of the whole inflorescence.Figure 1.


An improved embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species.

Li GR, Ji W, Wang G, Zhang JX, Wang YJ - In Vitro Cell. Dev. Biol., Plant (2013)

Embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species: (a) Inflorescence of female parent at anthesis and (b) at fruit set. (c–d) Ovules cultured in modified MM3 medium, and germinated embryos excised after 8 wk culture. (e) Whole plantlets developed from germinated embryos. (f) Tube plantlets transplanted to pots and sealed with transparent plastic cups. (g) Acclimation in a greenhouse. (h) Plants established in the field.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544466&req=5

Fig1: Embryo-rescue protocol for hybrid progeny from seedless Vitis vinifera grapes × wild Chinese Vitis species: (a) Inflorescence of female parent at anthesis and (b) at fruit set. (c–d) Ovules cultured in modified MM3 medium, and germinated embryos excised after 8 wk culture. (e) Whole plantlets developed from germinated embryos. (f) Tube plantlets transplanted to pots and sealed with transparent plastic cups. (g) Acclimation in a greenhouse. (h) Plants established in the field.
Mentions: Artificial pollination employed a soft brush loaded with dry pollen and was performed when the stigma mucus of the emasculated female parents was highest (Fig. 1a, b). Pollination was repeated three times on each of three consecutive days, followed by bagging of the whole inflorescence.Figure 1.

Bottom Line: The results indicated that Beichun and Shuangyou were the best male parents.The best sampling time for ovule inoculation differed among the female parents.Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar.

View Article: PubMed Central - PubMed

Affiliation: College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China ; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Yangling, Shaanxi 712100 People's Republic of China ; State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, Shaanxi 712100 People's Republic of China.

ABSTRACT

A highly efficient technique of embryo rescue is critical when using stenospermocarpic Vitis vinifera cultivars (female parents) to breed novel, disease-resistant, seedless grape cultivars by hybridizing with wild Chinese Vitis species (male parents) having many disease-resistance alleles. The effects of various factors on the improvement of embryo formation, germination, and plantlet development for seven hybrid combinations were studied. The results indicated that Beichun and Shuangyou were the best male parents. The best sampling time for ovule inoculation differed among the female parents. When hybrid ovules were cultured on a double-phase medium with five different solid medium types, percent embryo formation was highest (11.3-28.3%) on a modified MM3 medium. Percentages of embryo germination (15.4-55.4%) and plantlet development (11.15-44.6%) were all highest when embryos were cultured on Woody Plant Medium + 5.7 μM indole-3-acetic acid + 4.4 μM 6-benzylaminopurine + 1.4 μM gibberellic acid + 2% sucrose + 0.05% casein hydrolysate + 0.3% activated charcoal + 0.7% agar. In the absence of other amino acids, the addition of proline significantly increased embryo formation (36.1%), embryo germination (64.6%), and plantlet development (90.5%). A highly efficient protocol has been developed for hybrid embryo rescue from seedless V. vinifera grapes × wild Chinese Vitis species that results in a significant improvement in breeding efficiency for new disease-resistant seedless grapes.

No MeSH data available.


Related in: MedlinePlus