Limits...
GENOTYPE CHARACTERIZATION OF Leishmania (Viannia) braziliensis ISOLATED FROM HUMAN AND CANINE BIOPSIES WITH AMERICAN CUTANEOUS LEISHMANIASIS.

Ferreira LT, Gomes AH, Pereira-Chioccola VL - Rev. Inst. Med. Trop. Sao Paulo (2015 May-Jun)

Bottom Line: The results showed nine distinct patterns.These results suggest that polymorphic strains of L. (V.) braziliensis circulate in the state of São Paulo.These data agree with studies from other regions of Brazil, showing great variability among the natural populations of endemic foci.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia Molecular de Parasitas, Instituto Adolfo Lutz, São Paulo, SP, Brazil.

ABSTRACT

Introduction: American tegumentary leishmaniasis (ATL) can be caused by Leishmania (Viannia) braziliensis complex. The evolution of ATL initially results in lesions and can develop into disseminated or diffuse forms. The genetic diversity of L. (V.) braziliensis in some endemic areas of Brazil has been poorly studied, such as in the state of São Paulo. This study analyzed the genetic diversity of L. (V.) braziliensis isolates collected from patients and dogs with LTA from the state of São Paulo.

Methods: Leishmaniasis diagnosis was determined by PCR. The 132 biopsies were collected in different regions of Sao Paulo State, Brazil (36 municipalities). The genetic characterization of L. (V.) braziliensis isolates was tested by RFLP-PCR using DNA extracted from biopsies. The primer set amplified a specific region of Leishmania internal transcribed spacers of the ribosomal DNA locus.

Results: Of the 132 samples, 52 (40%) were completely genotyped by RFLP-PCR (44 from human patients and eight from dogs). The results showed nine distinct patterns. The majority of the genotyped samples were from Sorocaba (30), and the others were distributed among 14 other municipalities. The first pattern was more frequent (29 samples), followed by pattern 2 (nine samples) and pattern 3 (three samples). Patterns 4, 6, 7, 8 and 9 were composed of two samples each and pattern 5 of one sample.

Conclusion: These results suggest that polymorphic strains of L. (V.) braziliensis circulate in the state of São Paulo. These data agree with studies from other regions of Brazil, showing great variability among the natural populations of endemic foci.

Show MeSH

Related in: MedlinePlus

Map of South America and Brazil (A) indicating the location of the state ofSão Paulo (A). Map of the state of São Paulo (B) indicating the municipalitiesstudied and geographical distribution of the L. (V.)braziliensispatterns: 1 (red), 2 (yellow), 3 (green), 4 (blue), 5(purple), 6 (orange), 7 (pink), 8 (light blue) and 9 (gray).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4544252&req=5

f03: Map of South America and Brazil (A) indicating the location of the state ofSão Paulo (A). Map of the state of São Paulo (B) indicating the municipalitiesstudied and geographical distribution of the L. (V.)braziliensispatterns: 1 (red), 2 (yellow), 3 (green), 4 (blue), 5(purple), 6 (orange), 7 (pink), 8 (light blue) and 9 (gray).

Mentions: Pattern 1 was identical to those found in L. (V.) guyanensis andL. (V.) braziliensis WHO reference strains (Fig. 1). Furthermore, this L. (V.)braziliensispattern was the most common, since out of the 52 genotyped samples,29 (56%) belonged to pattern 1 and were distributed across 11 different municipalities.Pattern 2 was recurrent in nine samples distributed across three municipalities. Theother patterns (3 - 9) were uncommon and found in few samples: Pattern 3 (threemunicipalities), 4 (two municipalities), 5 (one municipality), 6 (one municipality), 7(one municipality), 8 (two municipalities), 9 (two municipalities), respectively. Thedetails and distribution of the clinical samples from the 44 human patients and eightdogs for each L. (V. ) braziliensis isolate are shown in Table 2 and Figure3. The majority (30) of the samples were from Sorocaba. The others (22) weredistributed across the other 14 municipalities.


GENOTYPE CHARACTERIZATION OF Leishmania (Viannia) braziliensis ISOLATED FROM HUMAN AND CANINE BIOPSIES WITH AMERICAN CUTANEOUS LEISHMANIASIS.

Ferreira LT, Gomes AH, Pereira-Chioccola VL - Rev. Inst. Med. Trop. Sao Paulo (2015 May-Jun)

Map of South America and Brazil (A) indicating the location of the state ofSão Paulo (A). Map of the state of São Paulo (B) indicating the municipalitiesstudied and geographical distribution of the L. (V.)braziliensispatterns: 1 (red), 2 (yellow), 3 (green), 4 (blue), 5(purple), 6 (orange), 7 (pink), 8 (light blue) and 9 (gray).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544252&req=5

f03: Map of South America and Brazil (A) indicating the location of the state ofSão Paulo (A). Map of the state of São Paulo (B) indicating the municipalitiesstudied and geographical distribution of the L. (V.)braziliensispatterns: 1 (red), 2 (yellow), 3 (green), 4 (blue), 5(purple), 6 (orange), 7 (pink), 8 (light blue) and 9 (gray).
Mentions: Pattern 1 was identical to those found in L. (V.) guyanensis andL. (V.) braziliensis WHO reference strains (Fig. 1). Furthermore, this L. (V.)braziliensispattern was the most common, since out of the 52 genotyped samples,29 (56%) belonged to pattern 1 and were distributed across 11 different municipalities.Pattern 2 was recurrent in nine samples distributed across three municipalities. Theother patterns (3 - 9) were uncommon and found in few samples: Pattern 3 (threemunicipalities), 4 (two municipalities), 5 (one municipality), 6 (one municipality), 7(one municipality), 8 (two municipalities), 9 (two municipalities), respectively. Thedetails and distribution of the clinical samples from the 44 human patients and eightdogs for each L. (V. ) braziliensis isolate are shown in Table 2 and Figure3. The majority (30) of the samples were from Sorocaba. The others (22) weredistributed across the other 14 municipalities.

Bottom Line: The results showed nine distinct patterns.These results suggest that polymorphic strains of L. (V.) braziliensis circulate in the state of São Paulo.These data agree with studies from other regions of Brazil, showing great variability among the natural populations of endemic foci.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia Molecular de Parasitas, Instituto Adolfo Lutz, São Paulo, SP, Brazil.

ABSTRACT

Introduction: American tegumentary leishmaniasis (ATL) can be caused by Leishmania (Viannia) braziliensis complex. The evolution of ATL initially results in lesions and can develop into disseminated or diffuse forms. The genetic diversity of L. (V.) braziliensis in some endemic areas of Brazil has been poorly studied, such as in the state of São Paulo. This study analyzed the genetic diversity of L. (V.) braziliensis isolates collected from patients and dogs with LTA from the state of São Paulo.

Methods: Leishmaniasis diagnosis was determined by PCR. The 132 biopsies were collected in different regions of Sao Paulo State, Brazil (36 municipalities). The genetic characterization of L. (V.) braziliensis isolates was tested by RFLP-PCR using DNA extracted from biopsies. The primer set amplified a specific region of Leishmania internal transcribed spacers of the ribosomal DNA locus.

Results: Of the 132 samples, 52 (40%) were completely genotyped by RFLP-PCR (44 from human patients and eight from dogs). The results showed nine distinct patterns. The majority of the genotyped samples were from Sorocaba (30), and the others were distributed among 14 other municipalities. The first pattern was more frequent (29 samples), followed by pattern 2 (nine samples) and pattern 3 (three samples). Patterns 4, 6, 7, 8 and 9 were composed of two samples each and pattern 5 of one sample.

Conclusion: These results suggest that polymorphic strains of L. (V.) braziliensis circulate in the state of São Paulo. These data agree with studies from other regions of Brazil, showing great variability among the natural populations of endemic foci.

Show MeSH
Related in: MedlinePlus