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SAINT LOUIS ENCEPHALITIS VIRUS IN MATO GROSSO, CENTRAL-WESTERN BRAZIL.

Heinen LB, Zuchi N, Serra OP, Cardoso BF, Gondim BH, Dos Santos MA, Souto FJ, Paula DA, Dutra V, Dezengrini-Slhessarenko R - Rev. Inst. Med. Trop. Sao Paulo (2015 May-Jun)

Bottom Line: Regarding mosquito samples, one pool containing one Culex quinquefasciatus female was positive for SLEV, with a minimum infection rate (MIR) of 0.29 per 1000 specimens of this species.Phylogenetic analysis indicates both human and mosquito SLEV cluster, with isolates from genotype V-A obtained from animals in the Amazon region, in the state of Pará.This is the first report of SLEV molecular identification in MT.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.

ABSTRACT
The dengue virus (DENV), which is frequently involved in large epidemics, and the yellow fever virus (YFV), which is responsible for sporadic sylvatic outbreaks, are considered the most important flaviviruses circulating in Brazil. Because of that, laboratorial diagnosis of acute undifferentiated febrile illness during epidemic periods is frequently directed towards these viruses, which may eventually hinder the detection of other circulating flaviviruses, including the Saint Louis encephalitis virus (SLEV), which is widely dispersed across the Americas. The aim of this study was to conduct a molecular investigation of 11 flaviviruses using 604 serum samples obtained from patients during a large dengue fever outbreak in the state of Mato Grosso (MT) between 2011 and 2012. Simultaneously, 3,433 female Culex spp. collected with Nasci aspirators in the city of Cuiabá, MT, in 2013, and allocated to 409 pools containing 1-10 mosquitoes, were also tested by multiplex semi-nested reverse transcription PCR for the same flaviviruses. SLEV was detected in three patients co-infected with DENV-4 from the cities of Cuiabá and Várzea Grande. One of them was a triple co-infection with DENV-1. None of them mentioned recent travel or access to sylvatic/rural regions, indicating that transmission might have occurred within the metropolitan area. Regarding mosquito samples, one pool containing one Culex quinquefasciatus female was positive for SLEV, with a minimum infection rate (MIR) of 0.29 per 1000 specimens of this species. Phylogenetic analysis indicates both human and mosquito SLEV cluster, with isolates from genotype V-A obtained from animals in the Amazon region, in the state of Pará. This is the first report of SLEV molecular identification in MT.

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Related in: MedlinePlus

Phylogenetic tree of envelope gene sequences from SLEV_BR/MT-CbaH364/2012,SLEV_BR/MT-CbaAr499/2013 and Saint Louis encephalitis virus (SLEV) genotypesdeposited at GenBank (NCBI), using the neighbor-joining method, Tamura-Neidistance model and 1,000 bootstrap replicates. Outgroups included Japaneseencephalitis virus (JEV), West Nile virus (WNV), and dengue virus 1(DENV-1).
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f02: Phylogenetic tree of envelope gene sequences from SLEV_BR/MT-CbaH364/2012,SLEV_BR/MT-CbaAr499/2013 and Saint Louis encephalitis virus (SLEV) genotypesdeposited at GenBank (NCBI), using the neighbor-joining method, Tamura-Neidistance model and 1,000 bootstrap replicates. Outgroups included Japaneseencephalitis virus (JEV), West Nile virus (WNV), and dengue virus 1(DENV-1).

Mentions: Phylogenetic analysis shows that SLEV identified in the present study within humans(SLEV_BR/MT-CbaH364/2012) and mosquitoes (SLEV_BR/MT-CbaAr499/2013) belong to genotypeV-A, closely related to isolates from animals in the Amazon region, in the state of Pará(Fig. 2). Previously, genotype VIII-B wasisolated from birds in the Amazon in MT in 197418.


SAINT LOUIS ENCEPHALITIS VIRUS IN MATO GROSSO, CENTRAL-WESTERN BRAZIL.

Heinen LB, Zuchi N, Serra OP, Cardoso BF, Gondim BH, Dos Santos MA, Souto FJ, Paula DA, Dutra V, Dezengrini-Slhessarenko R - Rev. Inst. Med. Trop. Sao Paulo (2015 May-Jun)

Phylogenetic tree of envelope gene sequences from SLEV_BR/MT-CbaH364/2012,SLEV_BR/MT-CbaAr499/2013 and Saint Louis encephalitis virus (SLEV) genotypesdeposited at GenBank (NCBI), using the neighbor-joining method, Tamura-Neidistance model and 1,000 bootstrap replicates. Outgroups included Japaneseencephalitis virus (JEV), West Nile virus (WNV), and dengue virus 1(DENV-1).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544245&req=5

f02: Phylogenetic tree of envelope gene sequences from SLEV_BR/MT-CbaH364/2012,SLEV_BR/MT-CbaAr499/2013 and Saint Louis encephalitis virus (SLEV) genotypesdeposited at GenBank (NCBI), using the neighbor-joining method, Tamura-Neidistance model and 1,000 bootstrap replicates. Outgroups included Japaneseencephalitis virus (JEV), West Nile virus (WNV), and dengue virus 1(DENV-1).
Mentions: Phylogenetic analysis shows that SLEV identified in the present study within humans(SLEV_BR/MT-CbaH364/2012) and mosquitoes (SLEV_BR/MT-CbaAr499/2013) belong to genotypeV-A, closely related to isolates from animals in the Amazon region, in the state of Pará(Fig. 2). Previously, genotype VIII-B wasisolated from birds in the Amazon in MT in 197418.

Bottom Line: Regarding mosquito samples, one pool containing one Culex quinquefasciatus female was positive for SLEV, with a minimum infection rate (MIR) of 0.29 per 1000 specimens of this species.Phylogenetic analysis indicates both human and mosquito SLEV cluster, with isolates from genotype V-A obtained from animals in the Amazon region, in the state of Pará.This is the first report of SLEV molecular identification in MT.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso, Cuiabá, Mato Grosso, Brazil.

ABSTRACT
The dengue virus (DENV), which is frequently involved in large epidemics, and the yellow fever virus (YFV), which is responsible for sporadic sylvatic outbreaks, are considered the most important flaviviruses circulating in Brazil. Because of that, laboratorial diagnosis of acute undifferentiated febrile illness during epidemic periods is frequently directed towards these viruses, which may eventually hinder the detection of other circulating flaviviruses, including the Saint Louis encephalitis virus (SLEV), which is widely dispersed across the Americas. The aim of this study was to conduct a molecular investigation of 11 flaviviruses using 604 serum samples obtained from patients during a large dengue fever outbreak in the state of Mato Grosso (MT) between 2011 and 2012. Simultaneously, 3,433 female Culex spp. collected with Nasci aspirators in the city of Cuiabá, MT, in 2013, and allocated to 409 pools containing 1-10 mosquitoes, were also tested by multiplex semi-nested reverse transcription PCR for the same flaviviruses. SLEV was detected in three patients co-infected with DENV-4 from the cities of Cuiabá and Várzea Grande. One of them was a triple co-infection with DENV-1. None of them mentioned recent travel or access to sylvatic/rural regions, indicating that transmission might have occurred within the metropolitan area. Regarding mosquito samples, one pool containing one Culex quinquefasciatus female was positive for SLEV, with a minimum infection rate (MIR) of 0.29 per 1000 specimens of this species. Phylogenetic analysis indicates both human and mosquito SLEV cluster, with isolates from genotype V-A obtained from animals in the Amazon region, in the state of Pará. This is the first report of SLEV molecular identification in MT.

Show MeSH
Related in: MedlinePlus