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FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL.

Menezes Rde P, Ferreira JC, de Sá WM, Moreira Tde A, Malvino LD, de Araujo LB, Röder DV, Penatti MP, Candido RC, Pedroso Rdos S - Rev. Inst. Med. Trop. Sao Paulo (2015 May-Jun)

Bottom Line: The two primers, OPA09 and OPB11, were able to distinguish different species.It was concluded that members of the C. parapsilosis complex were the most frequent species found, and most isolates were susceptible to the antifungals amphotericin B, flucozanole, and itraconazole.High genetic polymorphisms were observed for isolates of C. albicans and C. parapsilosis complex species, mainly with the OPA09 marker.

View Article: PubMed Central - PubMed

Affiliation: FAMED, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.

ABSTRACT
Infections by Candida species are a high-impact problem in public health due to their wide incidence in hospitalized patients. The goal of this study was to evaluate frequency, susceptibility to antifungals, and genetic polymorphism of Candida species isolated from clinical specimens of hospitalized patients. The Candida isolates included in this study were obtained from blood cultures, abdominal fluids, and central venous catheters (CVC) of hospitalized patients at the Clinical Hospital of the Federal University of Uberlândia during the period of July 2010 - June 2011. Susceptibility tests were conducted by the broth microdilution method. The RAPD-PCR tests used employed initiator oligonucleotides OPA09, OPB11, and OPE06. Of the 63 Candida isolates, 18 (28.5%) were C. albicans, 20 (31.7%) were C. parapsilosis complex species, 14 (22.2%) C. tropicalis, four (6.4%) C. glabrata, four (6.4%) C. krusei, two (3.3%) C. kefyr, and one (1.6%) C. lusitaniae. In vitro resistance to amphotericin B was observed in 12.7% of isolates. In vitro resistance to azoles was not detected, except for C. krusei. The two primers, OPA09 and OPB11, were able to distinguish different species. Isolates of C. albicans and C. parapsilosis complex species presented six and five clusters, respectively, with the OPA09 marker by RAPD-PCR, showing the genetic variability of the isolates of those species. It was concluded that members of the C. parapsilosis complex were the most frequent species found, and most isolates were susceptible to the antifungals amphotericin B, flucozanole, and itraconazole. High genetic polymorphisms were observed for isolates of C. albicans and C. parapsilosis complex species, mainly with the OPA09 marker.

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Distribution of Candida spp. according to age of hospitalized patients whodeveloped systemic candidiasis during the period of July 2010-June 2011.
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f01: Distribution of Candida spp. according to age of hospitalized patients whodeveloped systemic candidiasis during the period of July 2010-June 2011.

Mentions: During the study period, 63 cultures of body fluids from individuals with suspectedsystemic candidiasis were positive for Candida spp. , of which 47 werein blood, nine were in CVC, and seven in abdominal fluids, all obtained from 58hospitalized patients at the Clinical Hospital of Federal University of Uberlândia.Thirty-four were from males and 24 from females. Ages of the patients ranged from oneday to 94 years, with a mean age of 42 years. Most patients who developed systemiccandidiasis and who had a positive culture were older than or equal to 21 years (Fig. 1).


FREQUENCY OF Candida SPECIES IN A TERTIARY CARE HOSPITAL IN TRIANGULO MINEIRO, MINAS GERAIS STATE, BRAZIL.

Menezes Rde P, Ferreira JC, de Sá WM, Moreira Tde A, Malvino LD, de Araujo LB, Röder DV, Penatti MP, Candido RC, Pedroso Rdos S - Rev. Inst. Med. Trop. Sao Paulo (2015 May-Jun)

Distribution of Candida spp. according to age of hospitalized patients whodeveloped systemic candidiasis during the period of July 2010-June 2011.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544240&req=5

f01: Distribution of Candida spp. according to age of hospitalized patients whodeveloped systemic candidiasis during the period of July 2010-June 2011.
Mentions: During the study period, 63 cultures of body fluids from individuals with suspectedsystemic candidiasis were positive for Candida spp. , of which 47 werein blood, nine were in CVC, and seven in abdominal fluids, all obtained from 58hospitalized patients at the Clinical Hospital of Federal University of Uberlândia.Thirty-four were from males and 24 from females. Ages of the patients ranged from oneday to 94 years, with a mean age of 42 years. Most patients who developed systemiccandidiasis and who had a positive culture were older than or equal to 21 years (Fig. 1).

Bottom Line: The two primers, OPA09 and OPB11, were able to distinguish different species.It was concluded that members of the C. parapsilosis complex were the most frequent species found, and most isolates were susceptible to the antifungals amphotericin B, flucozanole, and itraconazole.High genetic polymorphisms were observed for isolates of C. albicans and C. parapsilosis complex species, mainly with the OPA09 marker.

View Article: PubMed Central - PubMed

Affiliation: FAMED, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil.

ABSTRACT
Infections by Candida species are a high-impact problem in public health due to their wide incidence in hospitalized patients. The goal of this study was to evaluate frequency, susceptibility to antifungals, and genetic polymorphism of Candida species isolated from clinical specimens of hospitalized patients. The Candida isolates included in this study were obtained from blood cultures, abdominal fluids, and central venous catheters (CVC) of hospitalized patients at the Clinical Hospital of the Federal University of Uberlândia during the period of July 2010 - June 2011. Susceptibility tests were conducted by the broth microdilution method. The RAPD-PCR tests used employed initiator oligonucleotides OPA09, OPB11, and OPE06. Of the 63 Candida isolates, 18 (28.5%) were C. albicans, 20 (31.7%) were C. parapsilosis complex species, 14 (22.2%) C. tropicalis, four (6.4%) C. glabrata, four (6.4%) C. krusei, two (3.3%) C. kefyr, and one (1.6%) C. lusitaniae. In vitro resistance to amphotericin B was observed in 12.7% of isolates. In vitro resistance to azoles was not detected, except for C. krusei. The two primers, OPA09 and OPB11, were able to distinguish different species. Isolates of C. albicans and C. parapsilosis complex species presented six and five clusters, respectively, with the OPA09 marker by RAPD-PCR, showing the genetic variability of the isolates of those species. It was concluded that members of the C. parapsilosis complex were the most frequent species found, and most isolates were susceptible to the antifungals amphotericin B, flucozanole, and itraconazole. High genetic polymorphisms were observed for isolates of C. albicans and C. parapsilosis complex species, mainly with the OPA09 marker.

Show MeSH
Related in: MedlinePlus