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Neuroprotective effects of Rosa damascena extract on learning and memory in a rat model of amyloid-β-induced Alzheimer's disease.

Esfandiary E, Karimipour M, Mardani M, Ghanadian M, Alaei HA, Mohammadnejad D, Esmaeili A - Adv Biomed Res (2015)

Bottom Line: Current medications only slow down the dementia progression and the present treatment one-drug one-target paradigm for anti-AD treatment appears to be clinically unsuccessful.Therefore, alternative therapeutic strategies are urgently needed.According to these results, we concluded that R. damascena can reverse behavioral deficits caused by A-β, and may provide a new potential option for prevention and treatment of the cognitive dysfunction in Alzheimer's disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Molecular Biology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: Alzheimer's disease (AD) is an age-related progressive neurodegenerative disease, which is characterized clinically by serious impairment in memory and cognition. Current medications only slow down the dementia progression and the present treatment one-drug one-target paradigm for anti-AD treatment appears to be clinically unsuccessful. Therefore, alternative therapeutic strategies are urgently needed. With respect to multifunctional and multitargeted characteristics of Rosa damascena via its effective flavonoids, we investigated the effects of R. damascena extract on behavioral functions in a rat model of amyloid-β (A-β)-induced Alzheimer's disease.

Materials and methods: After preparation of the methanolic extract of the R. damascena, HPLC analysis and toxicity studies, median lethal dose (LD50) and dose levels were determined. For evaluation of baseline training behavioral performance, Morris water maze and passive avoidance tests were used. A-β was injected bilaterally into CA1 area of the hippocampus. Twenty-one days after injection of A-β, the first probe trial of the behavioral tests were used to confirm learning and memory impairment. To examine the potential effects of the extract on behavioral tasks, the second probe trials were performed after one month administration of R. damasena extract.

Results: Results showed that the R. damascena extract significantly improved the spatial and long-term memories in the extract- treated groups in a dose-dependent manner, as in the middle and high doses it had significant effect.

Conclusion: According to these results, we concluded that R. damascena can reverse behavioral deficits caused by A-β, and may provide a new potential option for prevention and treatment of the cognitive dysfunction in Alzheimer's disease.

No MeSH data available.


Related in: MedlinePlus

Effects of the amyloid-β and Rosa damascena extract on spatial memory during the first (A, white color) and second (A, black color) probe trials, respectively, as measured by mean percentage time in target quadrant. **P < 0.01; ***P < 0.001 different from the control and sham groups. ##P < 0.01, ###P < 0.001 different from the Aβ-injected group (Alz + NS), @P < 0.05, @@@P < 0.001 different from the Aβ-injected group (Alz + 300)
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Figure 6: Effects of the amyloid-β and Rosa damascena extract on spatial memory during the first (A, white color) and second (A, black color) probe trials, respectively, as measured by mean percentage time in target quadrant. **P < 0.01; ***P < 0.001 different from the control and sham groups. ##P < 0.01, ###P < 0.001 different from the Aβ-injected group (Alz + NS), @P < 0.05, @@@P < 0.001 different from the Aβ-injected group (Alz + 300)

Mentions: Multivariate ANOVA analysis showed significant differences in 1st probe trial among groups (P < 0.001) [Figure 6]. Post hoc Tukey's HSD analysis showed that, the mean percentage of time spent in target quadrant in A-β-injected groups (3rd to 6th) after 21 days surgery was significantly decreased in comparison to control and sham groups (P < 0.001), but no significant differences were demonstrated in mean percentage of time spent in target quadrant between control and sham groups, (P = 0.9 > 0.05). These results showed that A-β–induced learning and memory impairment in the rats. Multivariate ANOVA analysis showed that mean percentage of time spent in target quadrant in 2nd probe trial after one month R. damascena extract treatment was significantly increased among groups (P < 0.001) [Figure 6]. Post hoc Tukey's HSD analysis showed that, mean percentage of time spent in target quadrant was significantly increased in extract-treated groups (5th and 6th groups) relative to 3rd group, which had received normal saline (P = 0.006 < 0.05, P < 0.001, respectively), but mean percentage of time in target quadrant in 4th group did not significantly increase relative to 3rd group (P = 0.3 > 0.05). Also mean percentage of time in target quadrant in 6th group was significantly increased in comparison to 4th (P = 0.002 < 0.05). Taken together, these results showed that R. damascena extract improved spatial learning and memory in a dose-dependent manner.


Neuroprotective effects of Rosa damascena extract on learning and memory in a rat model of amyloid-β-induced Alzheimer's disease.

Esfandiary E, Karimipour M, Mardani M, Ghanadian M, Alaei HA, Mohammadnejad D, Esmaeili A - Adv Biomed Res (2015)

Effects of the amyloid-β and Rosa damascena extract on spatial memory during the first (A, white color) and second (A, black color) probe trials, respectively, as measured by mean percentage time in target quadrant. **P < 0.01; ***P < 0.001 different from the control and sham groups. ##P < 0.01, ###P < 0.001 different from the Aβ-injected group (Alz + NS), @P < 0.05, @@@P < 0.001 different from the Aβ-injected group (Alz + 300)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544115&req=5

Figure 6: Effects of the amyloid-β and Rosa damascena extract on spatial memory during the first (A, white color) and second (A, black color) probe trials, respectively, as measured by mean percentage time in target quadrant. **P < 0.01; ***P < 0.001 different from the control and sham groups. ##P < 0.01, ###P < 0.001 different from the Aβ-injected group (Alz + NS), @P < 0.05, @@@P < 0.001 different from the Aβ-injected group (Alz + 300)
Mentions: Multivariate ANOVA analysis showed significant differences in 1st probe trial among groups (P < 0.001) [Figure 6]. Post hoc Tukey's HSD analysis showed that, the mean percentage of time spent in target quadrant in A-β-injected groups (3rd to 6th) after 21 days surgery was significantly decreased in comparison to control and sham groups (P < 0.001), but no significant differences were demonstrated in mean percentage of time spent in target quadrant between control and sham groups, (P = 0.9 > 0.05). These results showed that A-β–induced learning and memory impairment in the rats. Multivariate ANOVA analysis showed that mean percentage of time spent in target quadrant in 2nd probe trial after one month R. damascena extract treatment was significantly increased among groups (P < 0.001) [Figure 6]. Post hoc Tukey's HSD analysis showed that, mean percentage of time spent in target quadrant was significantly increased in extract-treated groups (5th and 6th groups) relative to 3rd group, which had received normal saline (P = 0.006 < 0.05, P < 0.001, respectively), but mean percentage of time in target quadrant in 4th group did not significantly increase relative to 3rd group (P = 0.3 > 0.05). Also mean percentage of time in target quadrant in 6th group was significantly increased in comparison to 4th (P = 0.002 < 0.05). Taken together, these results showed that R. damascena extract improved spatial learning and memory in a dose-dependent manner.

Bottom Line: Current medications only slow down the dementia progression and the present treatment one-drug one-target paradigm for anti-AD treatment appears to be clinically unsuccessful.Therefore, alternative therapeutic strategies are urgently needed.According to these results, we concluded that R. damascena can reverse behavioral deficits caused by A-β, and may provide a new potential option for prevention and treatment of the cognitive dysfunction in Alzheimer's disease.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomical Sciences and Molecular Biology, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.

ABSTRACT

Background: Alzheimer's disease (AD) is an age-related progressive neurodegenerative disease, which is characterized clinically by serious impairment in memory and cognition. Current medications only slow down the dementia progression and the present treatment one-drug one-target paradigm for anti-AD treatment appears to be clinically unsuccessful. Therefore, alternative therapeutic strategies are urgently needed. With respect to multifunctional and multitargeted characteristics of Rosa damascena via its effective flavonoids, we investigated the effects of R. damascena extract on behavioral functions in a rat model of amyloid-β (A-β)-induced Alzheimer's disease.

Materials and methods: After preparation of the methanolic extract of the R. damascena, HPLC analysis and toxicity studies, median lethal dose (LD50) and dose levels were determined. For evaluation of baseline training behavioral performance, Morris water maze and passive avoidance tests were used. A-β was injected bilaterally into CA1 area of the hippocampus. Twenty-one days after injection of A-β, the first probe trial of the behavioral tests were used to confirm learning and memory impairment. To examine the potential effects of the extract on behavioral tasks, the second probe trials were performed after one month administration of R. damasena extract.

Results: Results showed that the R. damascena extract significantly improved the spatial and long-term memories in the extract- treated groups in a dose-dependent manner, as in the middle and high doses it had significant effect.

Conclusion: According to these results, we concluded that R. damascena can reverse behavioral deficits caused by A-β, and may provide a new potential option for prevention and treatment of the cognitive dysfunction in Alzheimer's disease.

No MeSH data available.


Related in: MedlinePlus