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Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus

DU-145 cell growth pattern with different treatments at 6-, 12-, 24-, 48-, and 72-h time points
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Fig6: DU-145 cell growth pattern with different treatments at 6-, 12-, 24-, 48-, and 72-h time points

Mentions: Figure 6 shows the cytotoxicity of functionalized GNPs to the DU-145 cells at 1–6 h time points. A midrange concentration of 200 nm Mn-GNPs (50 μl) was used in this assay. After various binding assays, it was seen that 50 μl is the minimum concentration 200 nm Mn-GNPs required to effect binding. It is seen that mannose functionalized 200 nm GNPs when bound to EC-178 relatively show the same percent viability when compared to the control DU-145 cells. On the other hand, the EC-178 bacterial cells bring about cell death in about 6 h. When the 200 nm Mn-GNPs and EC178 are added together at once to the DU-145 cells, 64 % viability of the cell line is observed. But, when the 200 nm Mn-GNPs are premixed with EC-178 and then added to the DU-145 cell 73 % viability is observed. This shows that when they are premixed the functionalized GNPs bind to EC-178 and prevent the binding of the latter to DU-145, thereby increasing the cell viability. Even though the increase is not significant, it shows that there could be competitive binding between EC-178 and 200 nm Mn-GNPs to bind to DU-145 cells. This also suggests that the Mn-GNPs could competitively bind to the cell surface that has mannose residues and there by block the attachment of bacteria to those mannose residues on the DU-145 cell surface receptors (Miura et al. 2001).Fig. 6


Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

DU-145 cell growth pattern with different treatments at 6-, 12-, 24-, 48-, and 72-h time points
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544071&req=5

Fig6: DU-145 cell growth pattern with different treatments at 6-, 12-, 24-, 48-, and 72-h time points
Mentions: Figure 6 shows the cytotoxicity of functionalized GNPs to the DU-145 cells at 1–6 h time points. A midrange concentration of 200 nm Mn-GNPs (50 μl) was used in this assay. After various binding assays, it was seen that 50 μl is the minimum concentration 200 nm Mn-GNPs required to effect binding. It is seen that mannose functionalized 200 nm GNPs when bound to EC-178 relatively show the same percent viability when compared to the control DU-145 cells. On the other hand, the EC-178 bacterial cells bring about cell death in about 6 h. When the 200 nm Mn-GNPs and EC178 are added together at once to the DU-145 cells, 64 % viability of the cell line is observed. But, when the 200 nm Mn-GNPs are premixed with EC-178 and then added to the DU-145 cell 73 % viability is observed. This shows that when they are premixed the functionalized GNPs bind to EC-178 and prevent the binding of the latter to DU-145, thereby increasing the cell viability. Even though the increase is not significant, it shows that there could be competitive binding between EC-178 and 200 nm Mn-GNPs to bind to DU-145 cells. This also suggests that the Mn-GNPs could competitively bind to the cell surface that has mannose residues and there by block the attachment of bacteria to those mannose residues on the DU-145 cell surface receptors (Miura et al. 2001).Fig. 6

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus