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Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus

DU-145 cell uptake of plain GNPs (P-GNP) and mannose functionalized GNPs (Mn-GNPs). ***p < 0.001
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Fig5: DU-145 cell uptake of plain GNPs (P-GNP) and mannose functionalized GNPs (Mn-GNPs). ***p < 0.001

Mentions: After exposure to 20 and 200 nm plain and Mn-GNPs to DU-145 cells for 3 h, the average number of GNPs per cell associated with each DU-145 cell was estimated as shown in Fig. 5. The number of GNPs per cell in DU-145 cells was 3.4 × 104 for plain 20 nm GNP where as it was 1.7 × 104 for 200 nm plain GNPs. In contrast, exposure to 20 nm Mn-GNPs results in as much twice the increase of nanoparticle uptake compared to the plain 20 nm plain GNPs. There was a significant increase in the uptake of the 200 nm Mn-GNPs, more than double the number of GNPs when compared to the plain 200 nm GNPs. There does not seem to be a significant difference between uptake levels of Mn-GNPs (both 20 and 200 nm).Fig. 5


Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

DU-145 cell uptake of plain GNPs (P-GNP) and mannose functionalized GNPs (Mn-GNPs). ***p < 0.001
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544071&req=5

Fig5: DU-145 cell uptake of plain GNPs (P-GNP) and mannose functionalized GNPs (Mn-GNPs). ***p < 0.001
Mentions: After exposure to 20 and 200 nm plain and Mn-GNPs to DU-145 cells for 3 h, the average number of GNPs per cell associated with each DU-145 cell was estimated as shown in Fig. 5. The number of GNPs per cell in DU-145 cells was 3.4 × 104 for plain 20 nm GNP where as it was 1.7 × 104 for 200 nm plain GNPs. In contrast, exposure to 20 nm Mn-GNPs results in as much twice the increase of nanoparticle uptake compared to the plain 20 nm plain GNPs. There was a significant increase in the uptake of the 200 nm Mn-GNPs, more than double the number of GNPs when compared to the plain 200 nm GNPs. There does not seem to be a significant difference between uptake levels of Mn-GNPs (both 20 and 200 nm).Fig. 5

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus