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Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus

Cell cytotoxicity profiles of DU-145 cells in log phase. a Cell viability of DU-145 in log phase when treated with plain 20 nm GNPs and b cell viability of DU-145 in log phase when treated with 200 nm GNPs. *p < 0.05, ***p < 0.001
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Fig3: Cell cytotoxicity profiles of DU-145 cells in log phase. a Cell viability of DU-145 in log phase when treated with plain 20 nm GNPs and b cell viability of DU-145 in log phase when treated with 200 nm GNPs. *p < 0.05, ***p < 0.001

Mentions: The cytotoxicity of 20 and 200 nm GNPs at the different time points in log phase is shown in Fig. 3. It is seen that the 20 nm GNPs seem to have a significant effect on the DU-145 cells only after 48 and 72 h of incubation period in all the three concentrations. Not much difference in viability is observed by 200 nm GNPs in the log phase when compared to 20 nm GNPs. It is seen that the size did not cause much decrease or increase in cell viability. In case of the stationary phase (Fig. 4), the cytotoxicity of 20 and 200 nm GNPs is significant as they show 24–31 % reduction in cell viability at midrange of concentration (50 μl) compared to other low and high concentrations. Hence, it was observed that the midrange of 50 μl volume of GNPs significantly affected the percent cell viability in both log and stationary phase.Fig. 3


Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

Cell cytotoxicity profiles of DU-145 cells in log phase. a Cell viability of DU-145 in log phase when treated with plain 20 nm GNPs and b cell viability of DU-145 in log phase when treated with 200 nm GNPs. *p < 0.05, ***p < 0.001
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Related In: Results  -  Collection

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Fig3: Cell cytotoxicity profiles of DU-145 cells in log phase. a Cell viability of DU-145 in log phase when treated with plain 20 nm GNPs and b cell viability of DU-145 in log phase when treated with 200 nm GNPs. *p < 0.05, ***p < 0.001
Mentions: The cytotoxicity of 20 and 200 nm GNPs at the different time points in log phase is shown in Fig. 3. It is seen that the 20 nm GNPs seem to have a significant effect on the DU-145 cells only after 48 and 72 h of incubation period in all the three concentrations. Not much difference in viability is observed by 200 nm GNPs in the log phase when compared to 20 nm GNPs. It is seen that the size did not cause much decrease or increase in cell viability. In case of the stationary phase (Fig. 4), the cytotoxicity of 20 and 200 nm GNPs is significant as they show 24–31 % reduction in cell viability at midrange of concentration (50 μl) compared to other low and high concentrations. Hence, it was observed that the midrange of 50 μl volume of GNPs significantly affected the percent cell viability in both log and stationary phase.Fig. 3

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus