Limits...
Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus

Cell growth curve of DU-145 using different cell-plating numbers
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4544071&req=5

Fig2: Cell growth curve of DU-145 using different cell-plating numbers

Mentions: In order to quantify the toxicity of the 20 and 200 nm GNPs in DU-145 cells, a growth curve was carried out. Since cells that actively grow and divide during the logarithmic growth should be more vulnerable to toxic metallic particles than cells that are nearing or at the stationary phase of cell culture. Thus, the growth curve was determined to estimate the logarithmic and stationary growth phases in relation to the number of cells seeded into each well of a 96-well culture plate (Fig. 2). It was observed that the cells reach the logarithmic phase by 3–4 days of incubation. By the eighth day, the stationary phase kicks in.Fig. 2


Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

Cell growth curve of DU-145 using different cell-plating numbers
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544071&req=5

Fig2: Cell growth curve of DU-145 using different cell-plating numbers
Mentions: In order to quantify the toxicity of the 20 and 200 nm GNPs in DU-145 cells, a growth curve was carried out. Since cells that actively grow and divide during the logarithmic growth should be more vulnerable to toxic metallic particles than cells that are nearing or at the stationary phase of cell culture. Thus, the growth curve was determined to estimate the logarithmic and stationary growth phases in relation to the number of cells seeded into each well of a 96-well culture plate (Fig. 2). It was observed that the cells reach the logarithmic phase by 3–4 days of incubation. By the eighth day, the stationary phase kicks in.Fig. 2

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus