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Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


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a DU-145 cells with EC-178 at 0 min, b DU-145 cells with EC-208, c DU-145 cells with EC-178 at 1 h, d bright field flourescent image of DU-145 cells with EC-178 at 2 h, e DU-145 cells with EC-178 at 3 h, f control DU-145 cells. ×200 magnification. Scale bar 10 μm
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Fig1: a DU-145 cells with EC-178 at 0 min, b DU-145 cells with EC-208, c DU-145 cells with EC-178 at 1 h, d bright field flourescent image of DU-145 cells with EC-178 at 2 h, e DU-145 cells with EC-178 at 3 h, f control DU-145 cells. ×200 magnification. Scale bar 10 μm

Mentions: The binding of EC-178 and 208 to DU-145 was studied for 1, 2, and 3 h. Phase contrast images were taken as shown in Fig. 1. It was seen that the EC-208 cells did not bind to DU-145 cells at all during all the three time periods which is expected as it serves as a negative control (Fig. 1b). On the contrary, the EC-178 cells tend to bind to the cell wall of the DU-145 cells at 2-h time period (Fig. 1d). After the 3-h time period, they appear to be inside the DU-145 cell line (Fig. 1e). Due to the wash step after time periods, it is seen that only few cells manage to bind and enter the cell line between 2 and 3 h.Fig. 1


Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.

Vedantam P, Huang G, Tzeng TR - Cancer Nanotechnol (2013)

a DU-145 cells with EC-178 at 0 min, b DU-145 cells with EC-208, c DU-145 cells with EC-178 at 1 h, d bright field flourescent image of DU-145 cells with EC-178 at 2 h, e DU-145 cells with EC-178 at 3 h, f control DU-145 cells. ×200 magnification. Scale bar 10 μm
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4544071&req=5

Fig1: a DU-145 cells with EC-178 at 0 min, b DU-145 cells with EC-208, c DU-145 cells with EC-178 at 1 h, d bright field flourescent image of DU-145 cells with EC-178 at 2 h, e DU-145 cells with EC-178 at 3 h, f control DU-145 cells. ×200 magnification. Scale bar 10 μm
Mentions: The binding of EC-178 and 208 to DU-145 was studied for 1, 2, and 3 h. Phase contrast images were taken as shown in Fig. 1. It was seen that the EC-208 cells did not bind to DU-145 cells at all during all the three time periods which is expected as it serves as a negative control (Fig. 1b). On the contrary, the EC-178 cells tend to bind to the cell wall of the DU-145 cells at 2-h time period (Fig. 1d). After the 3-h time period, they appear to be inside the DU-145 cell line (Fig. 1e). Due to the wash step after time periods, it is seen that only few cells manage to bind and enter the cell line between 2 and 3 h.Fig. 1

Bottom Line: Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells.It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs.Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Clemson University, Clemson, SC 29634 USA.

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

No MeSH data available.


Related in: MedlinePlus