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Facile synthesis of biocompatible gold nanoparticles from Vites vinefera and its cellular internalization against HBL-100 cells.

Amarnath K, Mathew NL, Nellore J, Siddarth CR, Kumar J - Cancer Nanotechnol (2011)

Bottom Line: Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step.In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study.Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Dental College & Hospitals, Sathyabama University, Chennai, 600119 Tamil Nadu India.

ABSTRACT

The remarkable health benefits of the chemical cocktails occluded within Vites vinefera (grapes) have been broadly used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step. Furthermore, the grape-generated gold nanoparticles (GAuNPs), have demonstrated remarkable in vitro stability on specific functionalization with peptides (GSH) and thiol-containing compounds (lipoic acid) followed by the induction of cell-specific response. In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study. These studies thus signified the cellular internalization of GAuNPs and its conjugates by transmission electron microscopy through endocytosis into cancer cells. Notably, at higher concentration of gold nanoparticles conjugate, there was an asymmetric accumulation of gold nanoparticles in the periphery of the cell nucleus of the HBL-100 cells which was confirmed by fluorescence microscopy. Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

No MeSH data available.


Related in: MedlinePlus

Confocal microscopic image of control HBL-100 cells (a); HBL-100 cells treated with 500 μM of GSH-GAuNPs (b) arrows indicate agglomerated GAuNPs; c HBL-100 cells treated with 500 μM of LA-GAuNPs for 14 h. Arrows indicate agglomerated AuNPs in a membrane-bound vesicle (probably perinuclear lysosome)
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Fig9: Confocal microscopic image of control HBL-100 cells (a); HBL-100 cells treated with 500 μM of GSH-GAuNPs (b) arrows indicate agglomerated GAuNPs; c HBL-100 cells treated with 500 μM of LA-GAuNPs for 14 h. Arrows indicate agglomerated AuNPs in a membrane-bound vesicle (probably perinuclear lysosome)

Mentions: A very recent study by Midander and coworkers reported the effect of metal nanoparticles inducing single-stranded breaks in the human lung cells (Midander et al. 2009). Previous studies illustrated the potent cytotoxic, genotoxic, and toxicological activities of nanoparticles in vivo (Midander et al. 2009; Chen et al. 2006) and in cultured cancer cell lines (Sengupta et al. 2007). However, a methodical study using GAuNPs on DNA degradation and cytotoxicity towards breast cancer cells are missing up to date to the finest of our information. Thus, 40–80-nm-sized GSH-GAuNPs and LA-GAuNPs (lanes 2 and 3) treated HBL-100 cells in our study displayed a ladder pattern of inter-nucleosomal DNA fragmentation on TBE-agarose gel electrophoresis in DNA ladder assay (Studer et al. 2010) as revealed in Fig. 9 which is also another characteristic of apoptosis. All these results confirm that treatment with GSH-GAuNPs and LA-GAuNPs induce apoptosis in human breast cancer cells compared to GAuNPs.Fig. 9


Facile synthesis of biocompatible gold nanoparticles from Vites vinefera and its cellular internalization against HBL-100 cells.

Amarnath K, Mathew NL, Nellore J, Siddarth CR, Kumar J - Cancer Nanotechnol (2011)

Confocal microscopic image of control HBL-100 cells (a); HBL-100 cells treated with 500 μM of GSH-GAuNPs (b) arrows indicate agglomerated GAuNPs; c HBL-100 cells treated with 500 μM of LA-GAuNPs for 14 h. Arrows indicate agglomerated AuNPs in a membrane-bound vesicle (probably perinuclear lysosome)
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Related In: Results  -  Collection

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Fig9: Confocal microscopic image of control HBL-100 cells (a); HBL-100 cells treated with 500 μM of GSH-GAuNPs (b) arrows indicate agglomerated GAuNPs; c HBL-100 cells treated with 500 μM of LA-GAuNPs for 14 h. Arrows indicate agglomerated AuNPs in a membrane-bound vesicle (probably perinuclear lysosome)
Mentions: A very recent study by Midander and coworkers reported the effect of metal nanoparticles inducing single-stranded breaks in the human lung cells (Midander et al. 2009). Previous studies illustrated the potent cytotoxic, genotoxic, and toxicological activities of nanoparticles in vivo (Midander et al. 2009; Chen et al. 2006) and in cultured cancer cell lines (Sengupta et al. 2007). However, a methodical study using GAuNPs on DNA degradation and cytotoxicity towards breast cancer cells are missing up to date to the finest of our information. Thus, 40–80-nm-sized GSH-GAuNPs and LA-GAuNPs (lanes 2 and 3) treated HBL-100 cells in our study displayed a ladder pattern of inter-nucleosomal DNA fragmentation on TBE-agarose gel electrophoresis in DNA ladder assay (Studer et al. 2010) as revealed in Fig. 9 which is also another characteristic of apoptosis. All these results confirm that treatment with GSH-GAuNPs and LA-GAuNPs induce apoptosis in human breast cancer cells compared to GAuNPs.Fig. 9

Bottom Line: Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step.In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study.Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Dental College & Hospitals, Sathyabama University, Chennai, 600119 Tamil Nadu India.

ABSTRACT

The remarkable health benefits of the chemical cocktails occluded within Vites vinefera (grapes) have been broadly used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step. Furthermore, the grape-generated gold nanoparticles (GAuNPs), have demonstrated remarkable in vitro stability on specific functionalization with peptides (GSH) and thiol-containing compounds (lipoic acid) followed by the induction of cell-specific response. In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study. These studies thus signified the cellular internalization of GAuNPs and its conjugates by transmission electron microscopy through endocytosis into cancer cells. Notably, at higher concentration of gold nanoparticles conjugate, there was an asymmetric accumulation of gold nanoparticles in the periphery of the cell nucleus of the HBL-100 cells which was confirmed by fluorescence microscopy. Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

No MeSH data available.


Related in: MedlinePlus