Limits...
Facile synthesis of biocompatible gold nanoparticles from Vites vinefera and its cellular internalization against HBL-100 cells.

Amarnath K, Mathew NL, Nellore J, Siddarth CR, Kumar J - Cancer Nanotechnol (2011)

Bottom Line: Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step.In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study.Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Dental College & Hospitals, Sathyabama University, Chennai, 600119 Tamil Nadu India.

ABSTRACT

The remarkable health benefits of the chemical cocktails occluded within Vites vinefera (grapes) have been broadly used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step. Furthermore, the grape-generated gold nanoparticles (GAuNPs), have demonstrated remarkable in vitro stability on specific functionalization with peptides (GSH) and thiol-containing compounds (lipoic acid) followed by the induction of cell-specific response. In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study. These studies thus signified the cellular internalization of GAuNPs and its conjugates by transmission electron microscopy through endocytosis into cancer cells. Notably, at higher concentration of gold nanoparticles conjugate, there was an asymmetric accumulation of gold nanoparticles in the periphery of the cell nucleus of the HBL-100 cells which was confirmed by fluorescence microscopy. Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

No MeSH data available.


Related in: MedlinePlus

Schematic representation of biosynthesis of grape-based gold nanoparticles (Step 1) and ligand (GSH and lipoic acid) stabilized grape gold nanoparticles (Steps 2 and 3)
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4544069&req=5

Fig11: Schematic representation of biosynthesis of grape-based gold nanoparticles (Step 1) and ligand (GSH and lipoic acid) stabilized grape gold nanoparticles (Steps 2 and 3)

Mentions: Further, TEM images of breast tumor (HBL-100) cells treated with GSH-GAuNPs and LA-GAuNPs unequivocally validated our hypothesis. Significant internalization of GSH-GAuNPs and LA-GAuNPs via endocytosis within the HBL-100 cells was observed (Fig. 11). GSH-GAuNPs and LA-GAuNPs were detected within larger endocytic compartments of diverse morphology. These include peripherally both early and late endosomes and lysozomes. The internalization of nanoparticles within cells could occur via processes including phagocytosis, fluid-phase endocytosis, and receptor-mediated endocytosis. The viability of HBL-100 cells post-internalization suggests that the phytochemical coating and the size of the nanoparticles renders the nanoparticles nontoxic to cells. A number of studies have supported our study, demonstrating that phytochemicals have the ability to penetrate the cell membrane and internalize within the cellular matrix (Sun et al. 2007; Mizuno et al. 2007).Fig. 11


Facile synthesis of biocompatible gold nanoparticles from Vites vinefera and its cellular internalization against HBL-100 cells.

Amarnath K, Mathew NL, Nellore J, Siddarth CR, Kumar J - Cancer Nanotechnol (2011)

Schematic representation of biosynthesis of grape-based gold nanoparticles (Step 1) and ligand (GSH and lipoic acid) stabilized grape gold nanoparticles (Steps 2 and 3)
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4544069&req=5

Fig11: Schematic representation of biosynthesis of grape-based gold nanoparticles (Step 1) and ligand (GSH and lipoic acid) stabilized grape gold nanoparticles (Steps 2 and 3)
Mentions: Further, TEM images of breast tumor (HBL-100) cells treated with GSH-GAuNPs and LA-GAuNPs unequivocally validated our hypothesis. Significant internalization of GSH-GAuNPs and LA-GAuNPs via endocytosis within the HBL-100 cells was observed (Fig. 11). GSH-GAuNPs and LA-GAuNPs were detected within larger endocytic compartments of diverse morphology. These include peripherally both early and late endosomes and lysozomes. The internalization of nanoparticles within cells could occur via processes including phagocytosis, fluid-phase endocytosis, and receptor-mediated endocytosis. The viability of HBL-100 cells post-internalization suggests that the phytochemical coating and the size of the nanoparticles renders the nanoparticles nontoxic to cells. A number of studies have supported our study, demonstrating that phytochemicals have the ability to penetrate the cell membrane and internalize within the cellular matrix (Sun et al. 2007; Mizuno et al. 2007).Fig. 11

Bottom Line: Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step.In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study.Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Biochemistry, Dental College & Hospitals, Sathyabama University, Chennai, 600119 Tamil Nadu India.

ABSTRACT

The remarkable health benefits of the chemical cocktails occluded within Vites vinefera (grapes) have been broadly used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step. Furthermore, the grape-generated gold nanoparticles (GAuNPs), have demonstrated remarkable in vitro stability on specific functionalization with peptides (GSH) and thiol-containing compounds (lipoic acid) followed by the induction of cell-specific response. In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study. These studies thus signified the cellular internalization of GAuNPs and its conjugates by transmission electron microscopy through endocytosis into cancer cells. Notably, at higher concentration of gold nanoparticles conjugate, there was an asymmetric accumulation of gold nanoparticles in the periphery of the cell nucleus of the HBL-100 cells which was confirmed by fluorescence microscopy. Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.

No MeSH data available.


Related in: MedlinePlus