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Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus

Preservative effect of coated LDPE film during the storage of (a) meat (b) tomatoes. (a) Meat samples were spiked with L. monocytogenes (1–3) and S. aureus (4–6). Spoilage of meat is visible in meat samples packaged in control LDPE films (1 & 4) whereas no spoilage was observed in samples packaged with sonorensin (2 & 5) and nisin (3 & 6) coated LDPE films. (b) Tomato sample (1) packaged in untreated LDPE films showed signs of spoilage in contrast to no spoilage in case of tomatoes packaged in sonorensin (2) and nisin (3) coated LDPE films.
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f8: Preservative effect of coated LDPE film during the storage of (a) meat (b) tomatoes. (a) Meat samples were spiked with L. monocytogenes (1–3) and S. aureus (4–6). Spoilage of meat is visible in meat samples packaged in control LDPE films (1 & 4) whereas no spoilage was observed in samples packaged with sonorensin (2 & 5) and nisin (3 & 6) coated LDPE films. (b) Tomato sample (1) packaged in untreated LDPE films showed signs of spoilage in contrast to no spoilage in case of tomatoes packaged in sonorensin (2) and nisin (3) coated LDPE films.

Mentions: The sonorensin and nisin coated LDPE films were checked for their efficacy to inhibit the growth of food spoiling bacteria such as S. aureus and L. monocytogenes. Fresh meat spiked with these organisms and tomatoes were packed in sonoresin and nisin coated LDPE films and untreated LDPE films (control). The spoilage of both meat and tomatoes was observed in case of untreated packaging films after 4 days and 7 days of incubation at 4 oC respectively (Fig. 8). However, no signs of spoilage were seen in meat and tomatoes packed with sonorensin and nisin coated films (Fig. 8) even after 15 days of storage at refrigerated conditions. Moreover foul/stinky smell that was observed in meat samples packed with untreated films was absent in meat packed with sonorensin and nisin coated films. This suggested that like nisin, sonorensin could also be used as a food bio-preservative.


Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Preservative effect of coated LDPE film during the storage of (a) meat (b) tomatoes. (a) Meat samples were spiked with L. monocytogenes (1–3) and S. aureus (4–6). Spoilage of meat is visible in meat samples packaged in control LDPE films (1 & 4) whereas no spoilage was observed in samples packaged with sonorensin (2 & 5) and nisin (3 & 6) coated LDPE films. (b) Tomato sample (1) packaged in untreated LDPE films showed signs of spoilage in contrast to no spoilage in case of tomatoes packaged in sonorensin (2) and nisin (3) coated LDPE films.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544038&req=5

f8: Preservative effect of coated LDPE film during the storage of (a) meat (b) tomatoes. (a) Meat samples were spiked with L. monocytogenes (1–3) and S. aureus (4–6). Spoilage of meat is visible in meat samples packaged in control LDPE films (1 & 4) whereas no spoilage was observed in samples packaged with sonorensin (2 & 5) and nisin (3 & 6) coated LDPE films. (b) Tomato sample (1) packaged in untreated LDPE films showed signs of spoilage in contrast to no spoilage in case of tomatoes packaged in sonorensin (2) and nisin (3) coated LDPE films.
Mentions: The sonorensin and nisin coated LDPE films were checked for their efficacy to inhibit the growth of food spoiling bacteria such as S. aureus and L. monocytogenes. Fresh meat spiked with these organisms and tomatoes were packed in sonoresin and nisin coated LDPE films and untreated LDPE films (control). The spoilage of both meat and tomatoes was observed in case of untreated packaging films after 4 days and 7 days of incubation at 4 oC respectively (Fig. 8). However, no signs of spoilage were seen in meat and tomatoes packed with sonorensin and nisin coated films (Fig. 8) even after 15 days of storage at refrigerated conditions. Moreover foul/stinky smell that was observed in meat samples packed with untreated films was absent in meat packed with sonorensin and nisin coated films. This suggested that like nisin, sonorensin could also be used as a food bio-preservative.

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus