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Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus

Inhibitory activity of coated LDPE films against S. aureus.(a,c) control; (b) with sonorensin coated LDPE film; (d) with nisin coated LDPE film. The growth of S. aureus was inhibited by sonorensin and nisin coated LDPE films whereas S. aureus grew homogeneously on the surface of the plate and underneath the untreated LDPE film used as control.
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f7: Inhibitory activity of coated LDPE films against S. aureus.(a,c) control; (b) with sonorensin coated LDPE film; (d) with nisin coated LDPE film. The growth of S. aureus was inhibited by sonorensin and nisin coated LDPE films whereas S. aureus grew homogeneously on the surface of the plate and underneath the untreated LDPE film used as control.

Mentions: The low density polyethylene film (LDPE), coated with sonorensin and nisin, showed inhibitory activity against S. aureus (Fig. 7). Untreated film did not show any antimicrobial activity. As shown in Fig. 7, the superficial growth of indicator strain was limited to the area surrounding the activated film that could clearly inhibit the development of the S. aureus in contrast, it could grew homogeneously on the surface of the plate and underneath the untreated film used as control.


Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Inhibitory activity of coated LDPE films against S. aureus.(a,c) control; (b) with sonorensin coated LDPE film; (d) with nisin coated LDPE film. The growth of S. aureus was inhibited by sonorensin and nisin coated LDPE films whereas S. aureus grew homogeneously on the surface of the plate and underneath the untreated LDPE film used as control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544038&req=5

f7: Inhibitory activity of coated LDPE films against S. aureus.(a,c) control; (b) with sonorensin coated LDPE film; (d) with nisin coated LDPE film. The growth of S. aureus was inhibited by sonorensin and nisin coated LDPE films whereas S. aureus grew homogeneously on the surface of the plate and underneath the untreated LDPE film used as control.
Mentions: The low density polyethylene film (LDPE), coated with sonorensin and nisin, showed inhibitory activity against S. aureus (Fig. 7). Untreated film did not show any antimicrobial activity. As shown in Fig. 7, the superficial growth of indicator strain was limited to the area surrounding the activated film that could clearly inhibit the development of the S. aureus in contrast, it could grew homogeneously on the surface of the plate and underneath the untreated film used as control.

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus