Limits...
Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus

The scanning electron micrographs of S. aureus cells(a) without sonorensin treatment, and (b) after sonorensin treatment (50 μg/ml) for 4 h. The treatment of S. aureus with sonorensin displayed roughening of cell surface with cell debris while smooth cell surface was observed in cells without treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4544038&req=5

f6: The scanning electron micrographs of S. aureus cells(a) without sonorensin treatment, and (b) after sonorensin treatment (50 μg/ml) for 4 h. The treatment of S. aureus with sonorensin displayed roughening of cell surface with cell debris while smooth cell surface was observed in cells without treatment.

Mentions: To further gain insight into the mode of bactericidal action of the sonorensin, SEM of S. aureus treated with lethal dose of sonorensin was performed. When compared to untreated cells, S. aureus cells pre-incubated with 50 μg/ml of purified sonorensin for 4 h displayed major alterations like roughening of the cell surface with accumulation of cell debris and cell lysis (Fig. 6).


Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

The scanning electron micrographs of S. aureus cells(a) without sonorensin treatment, and (b) after sonorensin treatment (50 μg/ml) for 4 h. The treatment of S. aureus with sonorensin displayed roughening of cell surface with cell debris while smooth cell surface was observed in cells without treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544038&req=5

f6: The scanning electron micrographs of S. aureus cells(a) without sonorensin treatment, and (b) after sonorensin treatment (50 μg/ml) for 4 h. The treatment of S. aureus with sonorensin displayed roughening of cell surface with cell debris while smooth cell surface was observed in cells without treatment.
Mentions: To further gain insight into the mode of bactericidal action of the sonorensin, SEM of S. aureus treated with lethal dose of sonorensin was performed. When compared to untreated cells, S. aureus cells pre-incubated with 50 μg/ml of purified sonorensin for 4 h displayed major alterations like roughening of the cell surface with accumulation of cell debris and cell lysis (Fig. 6).

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus