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Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus

Flow cytometry analysis of effects of sonorensin and nisin on membrane integrity of S. aureus cells.Data were displayed as flow cytometric histograms of counted bacterial events (y-axis) associated cell fluorescence (x-axis). Marker M1 is the region that the damaged cells were stained by PI. (a) Unstained S. aureus cells (b) Untreated, PI stained S. aureus cells (c) Sonorensin treated, PI stained S. aureus cells, (d) nisin treated, PI stained S. aureus cells. For each sample 104 cells were analysed. The membrane integrity of S. aureus cells was destroyed by treatment with sonorensin.
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f5: Flow cytometry analysis of effects of sonorensin and nisin on membrane integrity of S. aureus cells.Data were displayed as flow cytometric histograms of counted bacterial events (y-axis) associated cell fluorescence (x-axis). Marker M1 is the region that the damaged cells were stained by PI. (a) Unstained S. aureus cells (b) Untreated, PI stained S. aureus cells (c) Sonorensin treated, PI stained S. aureus cells, (d) nisin treated, PI stained S. aureus cells. For each sample 104 cells were analysed. The membrane integrity of S. aureus cells was destroyed by treatment with sonorensin.

Mentions: PI is a viability-fluorescent marker that can penetrate impaired cells and intercalate into nucleic acid18. Sonorensin and nisin induced membrane damage of S. aureus cells was determined by staining the cells with PI after treatment with the sonorensin and nisin at 37 °C for 1 h using Flow Cytometer. As shown in Fig. 5, in the absence of any antibacterial agent, 76.5% of untreated control S. aureus cells showed no PI fluorescence signal. However, a significant increase in PI fluorescence was observed for the cells treated with sonorensin (70.0% cells stained with PI) and nisin (68.1% cells stained with PI) as depicted in Fig. 5. These results indicated that the membrane integrity of S. aureus cells was destroyed by treatment with sonorensin and the effectiveness was comparable to that of nisin.


Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Flow cytometry analysis of effects of sonorensin and nisin on membrane integrity of S. aureus cells.Data were displayed as flow cytometric histograms of counted bacterial events (y-axis) associated cell fluorescence (x-axis). Marker M1 is the region that the damaged cells were stained by PI. (a) Unstained S. aureus cells (b) Untreated, PI stained S. aureus cells (c) Sonorensin treated, PI stained S. aureus cells, (d) nisin treated, PI stained S. aureus cells. For each sample 104 cells were analysed. The membrane integrity of S. aureus cells was destroyed by treatment with sonorensin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544038&req=5

f5: Flow cytometry analysis of effects of sonorensin and nisin on membrane integrity of S. aureus cells.Data were displayed as flow cytometric histograms of counted bacterial events (y-axis) associated cell fluorescence (x-axis). Marker M1 is the region that the damaged cells were stained by PI. (a) Unstained S. aureus cells (b) Untreated, PI stained S. aureus cells (c) Sonorensin treated, PI stained S. aureus cells, (d) nisin treated, PI stained S. aureus cells. For each sample 104 cells were analysed. The membrane integrity of S. aureus cells was destroyed by treatment with sonorensin.
Mentions: PI is a viability-fluorescent marker that can penetrate impaired cells and intercalate into nucleic acid18. Sonorensin and nisin induced membrane damage of S. aureus cells was determined by staining the cells with PI after treatment with the sonorensin and nisin at 37 °C for 1 h using Flow Cytometer. As shown in Fig. 5, in the absence of any antibacterial agent, 76.5% of untreated control S. aureus cells showed no PI fluorescence signal. However, a significant increase in PI fluorescence was observed for the cells treated with sonorensin (70.0% cells stained with PI) and nisin (68.1% cells stained with PI) as depicted in Fig. 5. These results indicated that the membrane integrity of S. aureus cells was destroyed by treatment with sonorensin and the effectiveness was comparable to that of nisin.

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus