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Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus

The cytoplasmic membrane permeabilization of S. aureus cells treated with sonorensin (squares) and nisin (circles).The untreated S. aureus cells (triangles) were taken as control. When the cytoplasmic membrane was permeable ONPG entered the cytoplasm and degraded by β-galactosidase, producing O-nitrophenol that showed absorbance at 405 nm. Sonorensin induced an increase in the permeability of S. aureus. The experiment was carried out three times in triplicate. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).
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f4: The cytoplasmic membrane permeabilization of S. aureus cells treated with sonorensin (squares) and nisin (circles).The untreated S. aureus cells (triangles) were taken as control. When the cytoplasmic membrane was permeable ONPG entered the cytoplasm and degraded by β-galactosidase, producing O-nitrophenol that showed absorbance at 405 nm. Sonorensin induced an increase in the permeability of S. aureus. The experiment was carried out three times in triplicate. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).

Mentions: When the cytoplasmic membrane is permeable, ortho-Nitrophenyl-β-galactoside (ONPG), a non membrane—permeative chromogenic substrate, enters the cytoplasm and is degraded by β-galactosidase, producing O-nitrophenol that shows absorbance at 405 nm18. As shown in Fig. 4, sonorensin induced an increase in the permeability of S. aureus cytoplasmic membrane over time and in case of nisin (at same concentration), which has almost same MIC value against S. aureus, produced similar results of permeability. This suggested that sonorensin could permeabilize the cytoplasmic membrane of S. aureus.


Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

The cytoplasmic membrane permeabilization of S. aureus cells treated with sonorensin (squares) and nisin (circles).The untreated S. aureus cells (triangles) were taken as control. When the cytoplasmic membrane was permeable ONPG entered the cytoplasm and degraded by β-galactosidase, producing O-nitrophenol that showed absorbance at 405 nm. Sonorensin induced an increase in the permeability of S. aureus. The experiment was carried out three times in triplicate. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544038&req=5

f4: The cytoplasmic membrane permeabilization of S. aureus cells treated with sonorensin (squares) and nisin (circles).The untreated S. aureus cells (triangles) were taken as control. When the cytoplasmic membrane was permeable ONPG entered the cytoplasm and degraded by β-galactosidase, producing O-nitrophenol that showed absorbance at 405 nm. Sonorensin induced an increase in the permeability of S. aureus. The experiment was carried out three times in triplicate. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).
Mentions: When the cytoplasmic membrane is permeable, ortho-Nitrophenyl-β-galactoside (ONPG), a non membrane—permeative chromogenic substrate, enters the cytoplasm and is degraded by β-galactosidase, producing O-nitrophenol that shows absorbance at 405 nm18. As shown in Fig. 4, sonorensin induced an increase in the permeability of S. aureus cytoplasmic membrane over time and in case of nisin (at same concentration), which has almost same MIC value against S. aureus, produced similar results of permeability. This suggested that sonorensin could permeabilize the cytoplasmic membrane of S. aureus.

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus