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Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus

The scanning electron micrographs of mature (48 h old) biofilm of S. aureus cells (a) without sonorensin treatment and (b) after sonorensin treatment (50 μg/ml).The biofilms without sonorensin treatment consisted of nearly uniform, thick layer of cells embedded within a self produced matrix whereas thinning of mature biofilms was observed after sonorensin treatment.
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f2: The scanning electron micrographs of mature (48 h old) biofilm of S. aureus cells (a) without sonorensin treatment and (b) after sonorensin treatment (50 μg/ml).The biofilms without sonorensin treatment consisted of nearly uniform, thick layer of cells embedded within a self produced matrix whereas thinning of mature biofilms was observed after sonorensin treatment.

Mentions: Scanning electron micrographs of biofilms of S. aureus formed on cover slips and the effect of sonorensin on the preformed biofilm are shown in Fig. 2. For the non-treated controls, a biofilm formed consisted of nearly uniform, thick layer of cells (Fig. 2a), while the biofilm treated with sonorensin (50 μg/ml) was much less dense, and individually formed colonies could be seen (Fig. 2b).


Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

The scanning electron micrographs of mature (48 h old) biofilm of S. aureus cells (a) without sonorensin treatment and (b) after sonorensin treatment (50 μg/ml).The biofilms without sonorensin treatment consisted of nearly uniform, thick layer of cells embedded within a self produced matrix whereas thinning of mature biofilms was observed after sonorensin treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544038&req=5

f2: The scanning electron micrographs of mature (48 h old) biofilm of S. aureus cells (a) without sonorensin treatment and (b) after sonorensin treatment (50 μg/ml).The biofilms without sonorensin treatment consisted of nearly uniform, thick layer of cells embedded within a self produced matrix whereas thinning of mature biofilms was observed after sonorensin treatment.
Mentions: Scanning electron micrographs of biofilms of S. aureus formed on cover slips and the effect of sonorensin on the preformed biofilm are shown in Fig. 2. For the non-treated controls, a biofilm formed consisted of nearly uniform, thick layer of cells (Fig. 2a), while the biofilm treated with sonorensin (50 μg/ml) was much less dense, and individually formed colonies could be seen (Fig. 2b).

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus