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Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus

Effect of sonorensin on S. aureus biofilms.(a) Inhibition of attachment of S. aureus cells by sonorensin at various concentrations (b) Inhibition of biofilm formation (c) Viability of S. aureus cells biofilm (assayed by XTT). Control bars indicate S. aureus cells without any treatment taken as 100% in case of a & c and 0% in case of b. Each well of 96 -well plate contains 4 × 106 CFU S. aureus cells and variable concentration of sonorensin in 200 μl of BHI-sucrose. The plates were incubated at 37 °C for 4 h and 24 h for biofilm attachment assay (a) and inhibition of biofilm formation (b), respectively. (a) About 1.8 ± 0.05% attachment of biofilm was observed in the presence of 1X MIC of sonorensin. (b) Sonorensin showed significant inhibitory activity against S. aureus biofilm formation at 24 h, (c) Reduced XTT conversion was observed in wells with higher sonorensin concentrations and the control (without treatment) showed maximum reduction of XTT. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).
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f1: Effect of sonorensin on S. aureus biofilms.(a) Inhibition of attachment of S. aureus cells by sonorensin at various concentrations (b) Inhibition of biofilm formation (c) Viability of S. aureus cells biofilm (assayed by XTT). Control bars indicate S. aureus cells without any treatment taken as 100% in case of a & c and 0% in case of b. Each well of 96 -well plate contains 4 × 106 CFU S. aureus cells and variable concentration of sonorensin in 200 μl of BHI-sucrose. The plates were incubated at 37 °C for 4 h and 24 h for biofilm attachment assay (a) and inhibition of biofilm formation (b), respectively. (a) About 1.8 ± 0.05% attachment of biofilm was observed in the presence of 1X MIC of sonorensin. (b) Sonorensin showed significant inhibitory activity against S. aureus biofilm formation at 24 h, (c) Reduced XTT conversion was observed in wells with higher sonorensin concentrations and the control (without treatment) showed maximum reduction of XTT. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).

Mentions: When different concentrations of sonorensin were incubated with S. aureus for 4 h at 37 °C for adherence to the wells of microtiter plates, it inhibited biofilm attachment in a concentration dependent manner (Fig. 1a). About 1.8 ± 0.05% attachment of biofilm was observed in the presence of 1X MIC (~50 μg/ml) of sonorensin. Sonorensin showed significant inhibitory activity against S. aureus biofilm formation at 24 h, in relation to its concentration (Fig. 1b). When the sonorensin treated biofilms were subjected to 2, 3-Bis (2-methoxy-4-nitro-5-sulfophenyl)—2H-tetrazolium-5-carbox-anilide (XTT) assay, reduced XTT conversion was observed in wells with higher concentration of sonorensin while the negative control showed the maximum reduction of XTT indicating the effect of sonorensin on the viability of cells in biofilm (Fig. 1c).


Sonorensin: A new bacteriocin with potential of an anti-biofilm agent and a food biopreservative.

Chopra L, Singh G, Kumar Jena K, Sahoo DK - Sci Rep (2015)

Effect of sonorensin on S. aureus biofilms.(a) Inhibition of attachment of S. aureus cells by sonorensin at various concentrations (b) Inhibition of biofilm formation (c) Viability of S. aureus cells biofilm (assayed by XTT). Control bars indicate S. aureus cells without any treatment taken as 100% in case of a & c and 0% in case of b. Each well of 96 -well plate contains 4 × 106 CFU S. aureus cells and variable concentration of sonorensin in 200 μl of BHI-sucrose. The plates were incubated at 37 °C for 4 h and 24 h for biofilm attachment assay (a) and inhibition of biofilm formation (b), respectively. (a) About 1.8 ± 0.05% attachment of biofilm was observed in the presence of 1X MIC of sonorensin. (b) Sonorensin showed significant inhibitory activity against S. aureus biofilm formation at 24 h, (c) Reduced XTT conversion was observed in wells with higher sonorensin concentrations and the control (without treatment) showed maximum reduction of XTT. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4544038&req=5

f1: Effect of sonorensin on S. aureus biofilms.(a) Inhibition of attachment of S. aureus cells by sonorensin at various concentrations (b) Inhibition of biofilm formation (c) Viability of S. aureus cells biofilm (assayed by XTT). Control bars indicate S. aureus cells without any treatment taken as 100% in case of a & c and 0% in case of b. Each well of 96 -well plate contains 4 × 106 CFU S. aureus cells and variable concentration of sonorensin in 200 μl of BHI-sucrose. The plates were incubated at 37 °C for 4 h and 24 h for biofilm attachment assay (a) and inhibition of biofilm formation (b), respectively. (a) About 1.8 ± 0.05% attachment of biofilm was observed in the presence of 1X MIC of sonorensin. (b) Sonorensin showed significant inhibitory activity against S. aureus biofilm formation at 24 h, (c) Reduced XTT conversion was observed in wells with higher sonorensin concentrations and the control (without treatment) showed maximum reduction of XTT. The results were presented as mean ± SD and differences between the control and treated samples were statistically significant (n = 3) (p < 0.005).
Mentions: When different concentrations of sonorensin were incubated with S. aureus for 4 h at 37 °C for adherence to the wells of microtiter plates, it inhibited biofilm attachment in a concentration dependent manner (Fig. 1a). About 1.8 ± 0.05% attachment of biofilm was observed in the presence of 1X MIC (~50 μg/ml) of sonorensin. Sonorensin showed significant inhibitory activity against S. aureus biofilm formation at 24 h, in relation to its concentration (Fig. 1b). When the sonorensin treated biofilms were subjected to 2, 3-Bis (2-methoxy-4-nitro-5-sulfophenyl)—2H-tetrazolium-5-carbox-anilide (XTT) assay, reduced XTT conversion was observed in wells with higher concentration of sonorensin while the negative control showed the maximum reduction of XTT indicating the effect of sonorensin on the viability of cells in biofilm (Fig. 1c).

Bottom Line: Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus.Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability.The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

View Article: PubMed Central - PubMed

Affiliation: Biochemical Engineering Research and Process Development Centre, CSIR-Institute of Microbial Technology, Sector-39A Chandigarh; 160036, India.

ABSTRACT
The emergence of antibiotic resistant bacteria has led to exploration of alternative therapeutic agents such as ribosomally synthesized bacterial peptides known as bacteriocins. Biofilms, which are microbial communities that cause serious chronic infections, form environments that enhance antimicrobial resistance. Bacteria in biofilm can be upto thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. In this study, sonorensin, predicted to belong to the heterocycloanthracin subfamily of bacteriocins, was found to be effectively killing active and non-multiplying cells of both Gram-positive and Gram-negative bacteria. Sonorensin showed marked inhibition activity against biofilm of Staphylococcus aureus. Fluorescence and electron microscopy suggested that growth inhibition occurred because of increased membrane permeability. Low density polyethylene film coated with sonorensin was found to effectively control the growth of food spoilage bacteria like Listeria monocytogenes and S. aureus. The biopreservative effect of sonorensin coated film showing growth inhibition of spoilage bacteria in chicken meat and tomato samples demonstrated the potential of sonorensin as an alternative to current antibiotics/ preservatives.

No MeSH data available.


Related in: MedlinePlus