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A Bright Fluorescent Probe for H2S Enables Analyte-Responsive, 3D Imaging in Live Zebrafish Using Light Sheet Fluorescence Microscopy.

Hammers MD, Taormina MJ, Cerda MM, Montoya LA, Seidenkranz DT, Parthasarathy R, Pluth MD - J. Am. Chem. Soc. (2015)

Bottom Line: As our understanding of the complexity of physiological H2S in signaling pathways evolves, advanced chemical and technological investigative tools are required to make sense of this interconnectivity.Toward this goal, we have developed an azide-functionalized O-methylrhodol fluorophore, MeRho-Az, which exhibits a rapid >1000-fold fluorescence response when treated with H2S, is selective for H2S over other biological analytes, and has a detection limit of 86 nM.Additionally, the MeRho-Az scaffold is less susceptible to photoactivation than other commonly used azide-based systems, increasing its potential application in imaging experiments.

View Article: PubMed Central - PubMed

Affiliation: †Department of Chemistry and Biochemistry, ‡Department of Physics, §Institute of Molecular Biology, ∥Materials Science Institute. University of Oregon, Eugene, Oregon 97403-1253, United States.

ABSTRACT
Hydrogen sulfide (H2S) is a critical gaseous signaling molecule emerging at the center of a rich field of chemical and biological research. As our understanding of the complexity of physiological H2S in signaling pathways evolves, advanced chemical and technological investigative tools are required to make sense of this interconnectivity. Toward this goal, we have developed an azide-functionalized O-methylrhodol fluorophore, MeRho-Az, which exhibits a rapid >1000-fold fluorescence response when treated with H2S, is selective for H2S over other biological analytes, and has a detection limit of 86 nM. Additionally, the MeRho-Az scaffold is less susceptible to photoactivation than other commonly used azide-based systems, increasing its potential application in imaging experiments. To demonstrate the efficacy of this probe for H2S detection, we demonstrate the ability of MeRho-Az to detect differences in H2S levels in C6 cells and those treated with AOAA, a common inhibitor of enzymatic H2S synthesis. Expanding the use of MeRho-Az to complex and heterogeneous biological settings, we used MeRho-Az in combination with light sheet fluorescence microscopy (LSFM) to visualize H2S in the intestinal tract of live zebrafish. This application provides the first demonstration of analyte-responsive 3D imaging with LSFM, highlighting the utility of combining new probes and live imaging methods for investigating chemical signaling in complex multicellular systems.

No MeSH data available.


Integrated MeRho fluorescence (20 μM, λex = 476nm, λem = 480–650 nm) in aqueoussolution at various pH values (100 mM KCl).
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fig1: Integrated MeRho fluorescence (20 μM, λex = 476nm, λem = 480–650 nm) in aqueoussolution at various pH values (100 mM KCl).

Mentions: With both MeRho and MeRho-Az in hand,the photophysical properties of each compound were characterized (Table 1). MeRho displays excellent solubilityin aqueous buffer (50 mM PIPES, 100 mM KCl, pH 7.4), with absorptionand fluorescence bands centered at 476 and 516 nm, respectively (Figure S1). As predicted, MeRho exhibitsa high quantum yield (ΦMeRho = 0.57), whereas thequantum yield of the closed lactone form of MeRho-Az isessentially zero when excited at either the λmax (286nm) or that of MeRho (476 nm). To establish the fidelityof the MeRho scaffold under physiological conditions,we investigated the pH-dependent fluorescence. By performing a pHtitration and monitoring the fluorescence, we established that the MeRho fluorophore maintains a constant emission between pH4.5 and 10 (Figure 1), with apparent pKa values of 3.3 and 12.2, making the biologicallyviable pH range superior to that of fluorescein (Figure S2). Additionally, the MeRho fluorophoremaintains 75% of its maximum fluorescence under highly acidic conditions.


A Bright Fluorescent Probe for H2S Enables Analyte-Responsive, 3D Imaging in Live Zebrafish Using Light Sheet Fluorescence Microscopy.

Hammers MD, Taormina MJ, Cerda MM, Montoya LA, Seidenkranz DT, Parthasarathy R, Pluth MD - J. Am. Chem. Soc. (2015)

Integrated MeRho fluorescence (20 μM, λex = 476nm, λem = 480–650 nm) in aqueoussolution at various pH values (100 mM KCl).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543995&req=5

fig1: Integrated MeRho fluorescence (20 μM, λex = 476nm, λem = 480–650 nm) in aqueoussolution at various pH values (100 mM KCl).
Mentions: With both MeRho and MeRho-Az in hand,the photophysical properties of each compound were characterized (Table 1). MeRho displays excellent solubilityin aqueous buffer (50 mM PIPES, 100 mM KCl, pH 7.4), with absorptionand fluorescence bands centered at 476 and 516 nm, respectively (Figure S1). As predicted, MeRho exhibitsa high quantum yield (ΦMeRho = 0.57), whereas thequantum yield of the closed lactone form of MeRho-Az isessentially zero when excited at either the λmax (286nm) or that of MeRho (476 nm). To establish the fidelityof the MeRho scaffold under physiological conditions,we investigated the pH-dependent fluorescence. By performing a pHtitration and monitoring the fluorescence, we established that the MeRho fluorophore maintains a constant emission between pH4.5 and 10 (Figure 1), with apparent pKa values of 3.3 and 12.2, making the biologicallyviable pH range superior to that of fluorescein (Figure S2). Additionally, the MeRho fluorophoremaintains 75% of its maximum fluorescence under highly acidic conditions.

Bottom Line: As our understanding of the complexity of physiological H2S in signaling pathways evolves, advanced chemical and technological investigative tools are required to make sense of this interconnectivity.Toward this goal, we have developed an azide-functionalized O-methylrhodol fluorophore, MeRho-Az, which exhibits a rapid >1000-fold fluorescence response when treated with H2S, is selective for H2S over other biological analytes, and has a detection limit of 86 nM.Additionally, the MeRho-Az scaffold is less susceptible to photoactivation than other commonly used azide-based systems, increasing its potential application in imaging experiments.

View Article: PubMed Central - PubMed

Affiliation: †Department of Chemistry and Biochemistry, ‡Department of Physics, §Institute of Molecular Biology, ∥Materials Science Institute. University of Oregon, Eugene, Oregon 97403-1253, United States.

ABSTRACT
Hydrogen sulfide (H2S) is a critical gaseous signaling molecule emerging at the center of a rich field of chemical and biological research. As our understanding of the complexity of physiological H2S in signaling pathways evolves, advanced chemical and technological investigative tools are required to make sense of this interconnectivity. Toward this goal, we have developed an azide-functionalized O-methylrhodol fluorophore, MeRho-Az, which exhibits a rapid >1000-fold fluorescence response when treated with H2S, is selective for H2S over other biological analytes, and has a detection limit of 86 nM. Additionally, the MeRho-Az scaffold is less susceptible to photoactivation than other commonly used azide-based systems, increasing its potential application in imaging experiments. To demonstrate the efficacy of this probe for H2S detection, we demonstrate the ability of MeRho-Az to detect differences in H2S levels in C6 cells and those treated with AOAA, a common inhibitor of enzymatic H2S synthesis. Expanding the use of MeRho-Az to complex and heterogeneous biological settings, we used MeRho-Az in combination with light sheet fluorescence microscopy (LSFM) to visualize H2S in the intestinal tract of live zebrafish. This application provides the first demonstration of analyte-responsive 3D imaging with LSFM, highlighting the utility of combining new probes and live imaging methods for investigating chemical signaling in complex multicellular systems.

No MeSH data available.