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Myth busting? Effects of embryo positioning and egg turning on hatching success in the water snake Natrix maura.

Aubret F, Blanvillain G, Kok PJ - Sci Rep (2015)

Bottom Line: Here, we used 338 eggs from 32 clutches of the water snake Natrix maura to (1) thoroughly describe natural clutch arrangement, (2) experimentally assess the effects of natural embryo positioning and (3) egg turning on embryo metabolism, hatching success, and hatchling phenotype.Egg turning did not significantly influence egg development, hatching success or hatchling phenotypes.However, post-birth mortality was significantly higher in turned (37.5%) compared to unturned (4.5%) embryos, providing support to the common belief that eggs should not be moved from their natural position.

View Article: PubMed Central - PubMed

Affiliation: Station d'Ecologie Expérimentale de Moulis, CNRS, 09200 Moulis, France.

ABSTRACT
It is a common belief that reptile eggs should not be turned after oviposition once the embryo has attached itself to the inner membrane of the shell as it might kill developing embryos. Here, we used 338 eggs from 32 clutches of the water snake Natrix maura to (1) thoroughly describe natural clutch arrangement, (2) experimentally assess the effects of natural embryo positioning and (3) egg turning on embryo metabolism, hatching success, and hatchling phenotype. Clutches contained, on average, 59% of embryos located at the top, 28% at the bottom, and 14% on a side of the egg. Larger females laid larger clutches with higher proportion of top located embryos. Top embryos displayed higher metabolic rates (heart rates), shorter incubation time, and produced lighter and shorter snakes than bottom embryos. Egg turning did not significantly influence egg development, hatching success or hatchling phenotypes. However, post-birth mortality was significantly higher in turned (37.5%) compared to unturned (4.5%) embryos, providing support to the common belief that eggs should not be moved from their natural position.

No MeSH data available.


A total of 110 eggs (N = 14 clutches) of the water snake were incubated in the exact position they were laid, including 77 top embryos (located at the top of the egg) and 33 bottom embryos (located at the bottom) and eggs regularly surveyed for their heart rates (i.e. metabolic rate).Heart rates were consistently higher throughout the incubation period in top (85.23 ± 3.33 bpm) compared to bottom embryos (83.04 ± 3.17 bpm—repeated measures Anova; interaction term F54, 324 = 1.14; P = 0.24; effect of embryo location F1, 54 = 7.29; P = 0.009). Means + SE are given.
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f4: A total of 110 eggs (N = 14 clutches) of the water snake were incubated in the exact position they were laid, including 77 top embryos (located at the top of the egg) and 33 bottom embryos (located at the bottom) and eggs regularly surveyed for their heart rates (i.e. metabolic rate).Heart rates were consistently higher throughout the incubation period in top (85.23 ± 3.33 bpm) compared to bottom embryos (83.04 ± 3.17 bpm—repeated measures Anova; interaction term F54, 324 = 1.14; P = 0.24; effect of embryo location F1, 54 = 7.29; P = 0.009). Means + SE are given.

Mentions: Heart rates were consistently higher throughout the incubation period in top (85.23 ± 3.33 bpm) compared to bottom embryos (83.04 ± 3.17 bpm—see Fig. 4). A General Linear Model with (1) mean heart rate as a continuous predictor, (2) snout-vent length and body mass at birth as dependent variables, and (3) embryo positioning and clutch of origin as categorical predictors yielded a marginally non-significant effect of embryo positioning F2, 38 = 3.11; P = 0.056—see Fig. 5). The difference in heart rates between top and bottom embryos significantly explained the variation in body mass and snout-vent length at birth (see Table 4).


Myth busting? Effects of embryo positioning and egg turning on hatching success in the water snake Natrix maura.

Aubret F, Blanvillain G, Kok PJ - Sci Rep (2015)

A total of 110 eggs (N = 14 clutches) of the water snake were incubated in the exact position they were laid, including 77 top embryos (located at the top of the egg) and 33 bottom embryos (located at the bottom) and eggs regularly surveyed for their heart rates (i.e. metabolic rate).Heart rates were consistently higher throughout the incubation period in top (85.23 ± 3.33 bpm) compared to bottom embryos (83.04 ± 3.17 bpm—repeated measures Anova; interaction term F54, 324 = 1.14; P = 0.24; effect of embryo location F1, 54 = 7.29; P = 0.009). Means + SE are given.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543940&req=5

f4: A total of 110 eggs (N = 14 clutches) of the water snake were incubated in the exact position they were laid, including 77 top embryos (located at the top of the egg) and 33 bottom embryos (located at the bottom) and eggs regularly surveyed for their heart rates (i.e. metabolic rate).Heart rates were consistently higher throughout the incubation period in top (85.23 ± 3.33 bpm) compared to bottom embryos (83.04 ± 3.17 bpm—repeated measures Anova; interaction term F54, 324 = 1.14; P = 0.24; effect of embryo location F1, 54 = 7.29; P = 0.009). Means + SE are given.
Mentions: Heart rates were consistently higher throughout the incubation period in top (85.23 ± 3.33 bpm) compared to bottom embryos (83.04 ± 3.17 bpm—see Fig. 4). A General Linear Model with (1) mean heart rate as a continuous predictor, (2) snout-vent length and body mass at birth as dependent variables, and (3) embryo positioning and clutch of origin as categorical predictors yielded a marginally non-significant effect of embryo positioning F2, 38 = 3.11; P = 0.056—see Fig. 5). The difference in heart rates between top and bottom embryos significantly explained the variation in body mass and snout-vent length at birth (see Table 4).

Bottom Line: Here, we used 338 eggs from 32 clutches of the water snake Natrix maura to (1) thoroughly describe natural clutch arrangement, (2) experimentally assess the effects of natural embryo positioning and (3) egg turning on embryo metabolism, hatching success, and hatchling phenotype.Egg turning did not significantly influence egg development, hatching success or hatchling phenotypes.However, post-birth mortality was significantly higher in turned (37.5%) compared to unturned (4.5%) embryos, providing support to the common belief that eggs should not be moved from their natural position.

View Article: PubMed Central - PubMed

Affiliation: Station d'Ecologie Expérimentale de Moulis, CNRS, 09200 Moulis, France.

ABSTRACT
It is a common belief that reptile eggs should not be turned after oviposition once the embryo has attached itself to the inner membrane of the shell as it might kill developing embryos. Here, we used 338 eggs from 32 clutches of the water snake Natrix maura to (1) thoroughly describe natural clutch arrangement, (2) experimentally assess the effects of natural embryo positioning and (3) egg turning on embryo metabolism, hatching success, and hatchling phenotype. Clutches contained, on average, 59% of embryos located at the top, 28% at the bottom, and 14% on a side of the egg. Larger females laid larger clutches with higher proportion of top located embryos. Top embryos displayed higher metabolic rates (heart rates), shorter incubation time, and produced lighter and shorter snakes than bottom embryos. Egg turning did not significantly influence egg development, hatching success or hatchling phenotypes. However, post-birth mortality was significantly higher in turned (37.5%) compared to unturned (4.5%) embryos, providing support to the common belief that eggs should not be moved from their natural position.

No MeSH data available.