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Cattle NK Cell Heterogeneity and the Influence of MHC Class I.

Allan AJ, Sanderson ND, Gubbins S, Ellis SA, Hammond JA - J. Immunol. (2015)

Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.

View Article: PubMed Central - PubMed

Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.

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Related in: MedlinePlus

MHC class I haplotype influences the number of KLR receptors transcribed on individual dilution cultures. Dilution cultures were separated by MHC class I haplotype (A14, filled bars, and A18, open bars) and assessed for the total number of transcribed genes (top left) and also transcription of each NK receptor gene group: KIR, KLR, and CKM. The frequency has been calculated based on a normalized percentage of the dilution cultures with statistical significance between MHC class I haplotypes determined by a Fisher exact test. *p < 0.05.
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fig06: MHC class I haplotype influences the number of KLR receptors transcribed on individual dilution cultures. Dilution cultures were separated by MHC class I haplotype (A14, filled bars, and A18, open bars) and assessed for the total number of transcribed genes (top left) and also transcription of each NK receptor gene group: KIR, KLR, and CKM. The frequency has been calculated based on a normalized percentage of the dilution cultures with statistical significance between MHC class I haplotypes determined by a Fisher exact test. *p < 0.05.

Mentions: As the transcription of individual receptors was influenced by MHC class I genotype, we compared the entire transcription profiles by MHC class I background. Of the 66 dilution cultures described in this study, we observed 52 different transcription phenotypes using our panel of 25 genes, with no single phenotype seen more than twice (data not shown). When separated by MHC class I background, the A14 dilution cultures transcribed fewer genes than the A18, which was due to significantly less KLR gene transcription (p < 0.05) and less transcription of the CKM genes, which approached significance (p = 0.12) (Fig. 6).


Cattle NK Cell Heterogeneity and the Influence of MHC Class I.

Allan AJ, Sanderson ND, Gubbins S, Ellis SA, Hammond JA - J. Immunol. (2015)

MHC class I haplotype influences the number of KLR receptors transcribed on individual dilution cultures. Dilution cultures were separated by MHC class I haplotype (A14, filled bars, and A18, open bars) and assessed for the total number of transcribed genes (top left) and also transcription of each NK receptor gene group: KIR, KLR, and CKM. The frequency has been calculated based on a normalized percentage of the dilution cultures with statistical significance between MHC class I haplotypes determined by a Fisher exact test. *p < 0.05.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4543905&req=5

fig06: MHC class I haplotype influences the number of KLR receptors transcribed on individual dilution cultures. Dilution cultures were separated by MHC class I haplotype (A14, filled bars, and A18, open bars) and assessed for the total number of transcribed genes (top left) and also transcription of each NK receptor gene group: KIR, KLR, and CKM. The frequency has been calculated based on a normalized percentage of the dilution cultures with statistical significance between MHC class I haplotypes determined by a Fisher exact test. *p < 0.05.
Mentions: As the transcription of individual receptors was influenced by MHC class I genotype, we compared the entire transcription profiles by MHC class I background. Of the 66 dilution cultures described in this study, we observed 52 different transcription phenotypes using our panel of 25 genes, with no single phenotype seen more than twice (data not shown). When separated by MHC class I background, the A14 dilution cultures transcribed fewer genes than the A18, which was due to significantly less KLR gene transcription (p < 0.05) and less transcription of the CKM genes, which approached significance (p = 0.12) (Fig. 6).

Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.

View Article: PubMed Central - PubMed

Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.

Show MeSH
Related in: MedlinePlus