Cattle NK Cell Heterogeneity and the Influence of MHC Class I.
Bottom Line: Cytokine stimulation differentially influenced the transcription of five receptors at the cell population level.Using dilution cultures, we found that a further seven receptors were differentially transcribed, including five predicted to recognize MHC class I.Moreover, there was a statistically significant reduction in killer cell lectin-like receptor mRNA expression between cultures with different CD2 phenotypes and from animals with different MHC class I haplotypes.
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.Show MeSH
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Mentions: As the transcription of individual receptors was influenced by MHC class I genotype, we compared the entire transcription profiles by MHC class I background. Of the 66 dilution cultures described in this study, we observed 52 different transcription phenotypes using our panel of 25 genes, with no single phenotype seen more than twice (data not shown). When separated by MHC class I background, the A14 dilution cultures transcribed fewer genes than the A18, which was due to significantly less KLR gene transcription (p < 0.05) and less transcription of the CKM genes, which approached significance (p = 0.12) (Fig. 6).
Affiliation: The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, United Kingdom.